Primary N-assimilation into Amino Acids in Arabidopsis

Coruzzi, Gloria M.

doi:10.1199/tab.0010

Cited by 114 publications

(125 citation statements)

References 71 publications

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“…Pesquisas de NICOLOSO et al (2003) demonstraram que a presença de sacarose nas doses de 30, 45 e 60g L -1 , dentre as cinco fontes de carbono utilizadas, proporcionou o maior crescimento em altura de brotações, número de segmentos nodais, biomassa de raiz e parte aérea de P. glomerata cultivada in vitro. Também tem sido caracterizada a existência de uma alta correlação, tanto em nível fisiológico como molecular, do metabolismo do carbono e do nitrogênio sobre o crescimento das plantas, sendo que a presença de carbono reduzido, principalmente sacarose, estimula a assimilação do nitrato (CORUZZI, 2003).…”

Section: Ciência Rural V36 N4 Jul-ago 2006unclassified

“…O metabolismo do carbono e do nitrogênio, analisados sob aspectos moleculares e fisiológicos, interagem com forte correlação, na qual a presença de carbono reduzido estimula tanto a expressão gênica como a atividade de enzimas da assimilação do nitrato (CORUZZI, 2003). AVILA et al (1998), analisando as respostas de três cultivares de batata (Solanum tuberosum) à concentração e fontes de N, constataram que o número de segmentos nodais, o comprimento das brotações e a biomassa aumentaram na cv.…”

Section: Ciência Rural V36 N4 Jul-ago 2006unclassified

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Sacarose e nitrogênio na multiplicação in vitro de Pfaffia glomerata (Spreng.) Pedersen

et al. 2006

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RESUMOPfaffia glomerata (Spreng.) INTRODUÇÃOPfaffia glomerata (Spreng.) Pedersen, conhecida como ginseng brasileiro, é uma das espécies da família Amaranthaceae de grande interesse comercial na forma de fitomedicamentos e de suplementos alimentares por possuir propriedades rejuvenescedoras, revitalizantes, inibidoras do crescimento de células cancerígenas, antidiabéticas, tônicas, afrodisíacas, entre outras. A intensa exploração predatória das reservas naturais justifica a elaboração de planos de manejo ou projetos de cultivo para P. glomerata (MONTANARI JÚNIOR, 1999). Neste sentido, várias pesquisas vêm sendo realizadas no intuito de desenvolver protocolos eficientes de micropropagação para essa espécie (NICOLOSO et al., 2001b;RUSSOWSKI & NICOLOSO, 2003;NICOLOSO et al., 2003;SKREBSKY et al., 2004

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Section: Ciência Rural V36 N4 Jul-ago 2006unclassified

Sacarose e nitrogênio na multiplicação in vitro de Pfaffia glomerata (Spreng.) Pedersen

et al. 2006

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“…Increased aspartic acid may be beneficial to the plant during cold as mutants of AAT, that decreased aspartic production, showed reduced growth phenotypes (Coruzzi, 2003;Lam et al, 1995). With the Icelandic and Swedish populations having more aspartic acid in cold leaves over the Welsh population, this may prove to be advantageous during cold acclimation and growth.…”

Section: Targeted Metabolite Profilingmentioning

confidence: 99%

Intraspecfic variation in cold-temperature metabolic phenotypes of Arabidopsis lyrata ssp. petraea

2008

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Atmospheric temperature is a key factor in determining the distribution of a plant species. Alongside this, plant populations growing at the margin of their range may exhibit traits that indicate genetic differentiation and adaptation to their local abiotic environment. We investigated whether geographically separated marginal populations of Arabidopsis lyrata ssp. petraea have distinct metabolic phenotypes associated with exposure to cold temperatures. Seeds of A. petraea were obtained from populations along a latitudinal gradient, namely Wales, Sweden and Iceland and grown in a controlled cabinet environment. Mannose, glucose, fructose, sucrose and raffinose concentrations were different between cold treatments and populations, especially in the Welsh population, but polyhydric alcohol concentrations were not.The free amino acid compositions were population specific, with fold differences in most amino acids, especially in the Icelandic populations, with gross changes in amino acids, particularly those associated with glutamine metabolism. Metabolic fingerprints and profiles were obtained. Principal component analysis (PCA) of metabolite fingerprints revealed metabolic characteristic phenotypes for each population and temperature. It is suggested that amino acids and carbohydrates were responsible for discriminating populations within the PCA. Metabolite fingerprinting and profiling has proved to be sufficiently sensitive to identify metabolic differences between plant populations at different atmospheric temperatures. These findings show that there is significant natural variation in cold metabolism among populations of A. l. petraea which may signify plant adaptation to local climates.

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“…AS consists of a and b subunits in plants and may function as a 2 b 2 (Coruzzi and Last 2000). The a subunit is deemed to play a crucial role for feedback inhibition; it has a Trp binding site that enables allosteric regulation.…”

Section: Manipulation Of Trp Contentmentioning

confidence: 99%

Metabolic engineering of the tryptophan and phenylalanine biosynthetic pathways in rice

Wakasa

Ishihara

2009

Plant Biotechnology

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Tryptophan (Trp) and phenylalanine (Phe) are essential aromatic amino acids that animals cannot synthesize and are dependent on plants for their supply. In particular, Trp contributes to the nutritional quality of plant-based foods, and, along with lysine, methionine and threonine, it is used to supplement animal feeds. The other aromatic amino acids, Phe and tyrosine (Tyr), are used for the production of the low-calorie sweetener aspartame and the anti-Parkinson's disease drug L-dopa, respectively. The biosynthetic pathways for aromatic amino acids and their regulation have been extensively explored in bacteria because of their utility in the food and drug industry. In plants, aromatic amino acids are the precursors for a large variety of secondary metabolites including indole alkaloids, phenylpropanoids, flavonoids, and the phenolic polymer, lignin. One of plant growth regulators, indole-3-acetic acid (IAA), has also been shown to be biosynthesized from Trp. Thus, aromatic amino acids also play important roles in plant defense and development.In bacteria, fungi and plants, the three aromatic amino acids, Trp, Phe and Tyr, are synthesized from a common precursor, chorismate that originates from the shikimate pathway ( Figure 1). In bacteria, this pathway is almost exclusively used to produce aromatic amino acids for protein synthesis but, in higher plants, this pathway leads to the production of numerous aromatic secondary metabolites. The importance of this pathway in plants is indicated by the fact that about 20% of the carbon fixed by photosynthesis flow through this pathway. These facts strongly suggest that the modification of the shikimate and its derivative (or downstream) pathways may lead to dramatic changes in secondary metabolism. The enzymatic reactions in the biosynthetic pathway for aromatic amino acids seem to be identical in prokaryotes and eukaryotes but they apparently differ in the regulatory mechanisms for these pathways. For example, 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase, the enzyme that catalyzes the first reaction in the shikimate pathway, is not feedback inhibited by any of the aromatic amino acids in plants, but, each of the three DAHP synthase isoenzymes are Abstract Aromatic amino acids function as building blocks of proteins and as precursors for secondary metabolism. To obtain plants that accumulate tryptophan (Trp) and phenylalanine (Phe), we modified the biosynthetic pathways for these amino acids in rice and dicot species. By introducing a gene encoding a feedback-insensitive anthranilate synthase (AS) alpha subunit, we successfully obtained transgenic plants that over-accumulated Trp. In addition, we found mutant calli that accumulated Phe and Trp at high concentrations. The causal gene (mtr1-D) encoded an arogenate dehydratase (ADT)/prephenate dehydratase (PDT) that catalyzes the final reaction in Phe biosynthesis. The wild-type enzyme was sensitive to feedback inhibition by Phe, but the mutant enzyme encoded by mtr1-D was relatively insensitive. Further, ...

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Primary N-assimilation into Amino Acids in Arabidopsis

Cited by 114 publications

References 71 publications

Sacarose e nitrogênio na multiplicação in vitro de Pfaffia glomerata (Spreng.) Pedersen

Sacarose e nitrogênio na multiplicação in vitro de Pfaffia glomerata (Spreng.) Pedersen

Intraspecfic variation in cold-temperature metabolic phenotypes of Arabidopsis lyrata ssp. petraea

Metabolic engineering of the tryptophan and phenylalanine biosynthetic pathways in rice

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