Primary stress responses in Arabidopsis thaliana exposed to gamma radiation

Vanhoudt, Nathalie; Horemans, Nele; Wannijn, Jean; Nauts, Robin; Hees, May Van; Vandenhove, Hildegarde

doi:10.1016/j.jenvrad.2013.11.011

Cited by 57 publications

(33 citation statements)

References 28 publications

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“…Similar results were described by Vanhoudt et al (2010Vanhoudt et al ( , 2014 in studies with A. thaliana and by Silva et al (2011) in rice, in which diff erent doses of gamma radiation also did not alter the activity of CAT.…”

supporting

confidence: 84%

“…There was a trend of reduced root length as the radiation dose increased, but significant differences were only noted between the control group and the 150 and 200 Gy treatments and between the 150 and 200 Gy treatments themselves (Figure 2d). Vanhoudt et al (2014) exposed Arabidopsis thaliana seedlings to different gamma radiation doses and observed changes in root and leaf fresh weights but did not observe dose-dependent growth inhibition. However, these authors used lower dose rates compared to those utilized in the current study.…”

Section: Seedling Vigor and Biomassmentioning

confidence: 96%

“…It is known that gamma radiation can cause alterations in enzyme capacities; however, the results reported by previous studies are dependent on the radiation dose and the plant species used (Silva et al 2011, Vanhoudt et al 2014. In the present study, we evaluated the SOD activity in castor bean seedling leaves.…”

mentioning

confidence: 94%

“…In plants, the antioxidant system regulates and adjusts ROS levels according to the needs of the cell. Gamma radiation causes ROS generation because water molecules present in the organism absorb the energy released by the radiation, causing its dissociation (Vanhoudt et al 2014). This results in the release of H + ions as well as H • and OH • free radicals, which have high chemical reactivity and tend to recombine to form ROS.…”

mentioning

confidence: 99%

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Morphophysiological and biochemical alterations in Ricinus communis L. seeds submitted to cobalt60 gamma radiation

Lopes

Bobrowski

Silva

et al. 2017

An. Acad. Bras. Ciênc.

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This study aimed to evaluate the radiosensitivity of castor bean seeds after applications of different doses of Cobalt 60 gamma radiation. Seeds were pre-soaked for 24 hours in distilled water and then irradiated with 50, 100, 150, and 200 Gy, except the control. Sowing was performed in trays, which contained soil as substrate and were maintained in a greenhouse. The electrical conductivity, emergence, emergence speed index, growth parameters and activities of antioxidant enzymes (superoxide dismutase, ascorbate peroxidase, and catalase) were evaluated in the leaves and roots of castor bean seedlings. Gamma radiation did not affect the electrical conductivity of the seeds; however, at a dose of 200 Gy, the emergence and emergence speed index of the seedlings was negatively affected. An analysis of the morphophysiological parameters revealed a reduction in seedling size as the radiation dose increased. There was a significant increase in superoxide dismutase and ascorbate peroxidase activities at higher radiation doses in the leaves, but not in roots. Thus, the analysis of all the variables suggests a response pattern as to the morphophysiological and biochemical changes of castor bean seedlings due to the increase of gamma radiation, which may serve as a tool for generating greater genetic variability.

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supporting

confidence: 84%

Section: Seedling Vigor and Biomassmentioning

confidence: 96%

mentioning

confidence: 94%

mentioning

confidence: 99%

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Morphophysiological and biochemical alterations in Ricinus communis L. seeds submitted to cobalt60 gamma radiation

Lopes

Bobrowski

Silva

et al. 2017

An. Acad. Bras. Ciênc.

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“…Superoxide dismutase (SOD) represents the first line of ROS defense as it catalyzes the disproportionation of superoxide into hydrogen peroxide (McCord and Fridovich, 1969). Catalase (CAT) and other antioxidases such as peroxidases (POD), ascorbate peroxidase (APX) and guaiacol peroxidase (GPX) function as important hydrogen peroxide scavengers (Bonet et al, 2012;Vanhoudt et al, 2014). To get an overview of the effects of different light irradiances on these ROS-scavenging enzymes, activities of SOD and CAT were determined.…”

Section: Discussionmentioning

confidence: 99%

Growth, pigment composition, chlorophyll fluorescence and antioxidant defenses in the red alga Gracilaria lemaneiformis (Gracilariales, Rhodophyta) under light stress

Jiang

Liu

et al. 2015

South African Journal of Botany

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a b s t r a c t Edited by J Van StadenKeywords: Gracilaria lemaneiformis photosynthesis Chlorophyll fluorescence Antioxidative enzymes Light intensityTo investigate the response of the Gracilaria lemaneiformis (Gracilariales, Rhodophyta) to light stress, algae were grown at low (LL), intermediate (IL), and high light (HL) conditions. The relationship between photoacclimatory plasticity, light energy utilization and antioxidant capacity, and many physiological and biochemical parameters, including growth rate, pigment composition, ratio of variable fluorescence to maximum fluorescence (Fv/Fm), photochemical quenching coefficient (qP), non-photochemical quenching coefficient (NPQ), superoxide dismutase (SOD) and catalase (CAT) were determined in this study. With the increase in light intensity from LL to HL, the RGR, Chl a, Car contents, and maximal photochemical efficiency of photosystem II (PSII) increased significantly at 72 h and 120 h. However, algae growing at LL exhibited reduced photochemical efficiency of PSII and physiological activity as compared to that at HL, that is characterized by NPQ, SOD and CAT enhancement, but Chl a measuring at LL was significant higher than that at IL. Meanwhile, high Chl a value was also observed at HL after 72 h and 120 h culture compared with that at ML. The results suggest that under the low light irradiance, the G. lemaneiformis performed a negative vegetal and physiological strategy for adapting the environmental stress via regulating PSII reaction centers normalization and ROS metabolization, and so forth.

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Orange peels and Chlorella vulgaris supplementation ameliorate gamma radiation‐induced oxidative stress by regulating TGF‐β and NOX2/NOX4 signaling pathways

Abdelrahman,

Mohammed,

Badawy

et al. 2023

Cell Biochemistry & Function

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Numerous studies revealed that Chlorella vulgaris and orange peels are potential sources for many valuable compounds such as flavonoids, which are natural polyphenols with antioxidant capacities that lessen oxidative stress via suppressing ROS levels. Thus, this study was designed to investigate their radioprotective efficiency either alone or in combination as natural food supplements. Sixty‐four male Albino rats were divided into eight groups (n = 8) as follows: control, orange peel (10% in diet), C. vulgaris (1% in diet), orange peel + C. vulgaris, gamma irradiated (2Gy twice per week up to 8Gy), orange peel + gamma irradiation, C. vulgaris + gamma irradiation, and orange peel + C. vulgaris + gamma irradiation. After the experiment, blood serums were collected for biochemical analysis, whole bloods were collected for blood picture, bone marrows were collected for GSH, MDA, TGF‐β, NOX2 and NOX4, and liver tissues were collected for histopathological evaluation. Current study revealed that exposure to gamma irradiation induced a significant disturbance in liver function markers (ALT and AST), kidney function markers (urea and creatinine), cholesterol and triglycerides levels in serum. In addition, a significant decrease in WBCs, RBCs, PLT, and Hb in blood of irradiated rats. Moreover, a significant elevation in TGF‐β, NOX2, NOX4 activities, and MDA level, while showed a marked decrease in GSH concentration. Furthermore, hepatic inflammation appeared in the histopathological examination. Orange peels or C. vulgaris treatments showed acceptable amelioration in all measured parameters, combination between orange peels and C. vulgaris showed statistically significant additive amelioration in radiation induced disturbance.

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Primary stress responses in Arabidopsis thaliana exposed to gamma radiation

Cited by 57 publications

References 28 publications

Morphophysiological and biochemical alterations in Ricinus communis L. seeds submitted to cobalt60 gamma radiation

Morphophysiological and biochemical alterations in Ricinus communis L. seeds submitted to cobalt60 gamma radiation

Growth, pigment composition, chlorophyll fluorescence and antioxidant defenses in the red alga Gracilaria lemaneiformis (Gracilariales, Rhodophyta) under light stress

Orange peels and Chlorella vulgaris supplementation ameliorate gamma radiation‐induced oxidative stress by regulating TGF‐β and NOX2/NOX4 signaling pathways

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