Primary structure and androgen regulation of a 20-kilodalton protein specific to rat ventral prostate

Abstract

Nuclear and cytosolic forms of a 20-kdalton rat ventral prostate protein were purified and partially sequenced from their N-termini. Isolated nuclei were treated with micrococcal nuclease and extracted in 0.6 M NaCl, and proteins were separated by affinity chromatography on Matrex gel green A, ammonium sulfate fractionation, and fast protein liquid chromatography on Superose 12. The 43 amino acid N-terminal sequence of the nuclear 20-kdalton protein was identical with the cytosolic protein except it lacked 7 N-terminal amino acids present in the cytosolic form. The DNA sequence of a full-length complementary DNA clone isolated from a ventral prostate gt11 library extended the N-terminal sequence of the cytosolic form by an additional nine amino acids from the predicted initiation methionine. The cDNA included the nucleotide sequence for the 43 amino acid N-terminal sequence of the purified 20-kdalton protein and predicted molecular weights of 16,686, 17,521, and 18,650, respectively, for the nuclear, cytoplasmic, and nonprocessed proteins. Northern blot analyses of reproductive tract tissue RNAs using the 20-kdalton protein cDNA as probe revealed a single mRNA species of 0.92 kb detectable only in extracts of rat ventral prostate. Expression of the 0.92-kb mRNA was androgen dependent since the mRNA was undetectable in extracts obtained 4 days after castration and was restored 16 h after restimulation with androgen.

Detachment activity of human saliva in vitro for Candida albicans cells attached to a plastic plate

“…14 Trypsin treatment of saliva Trypsin (Sigma Chemical, St. Louis, MO, USA) in RPMI 1640 medium containing 1% FCS was added to the human saliva, to achieve a final trypsin concentration of 0.025%. This saliva was then incubated at 4°C for 1 h and at 37°C for 1 h for comparison purposes.…”

Biological function and mode of action of the androgen receptor

“…Others have more complex sequences. A number of androgen-regulated genes have been identified that contain simple or complex androgen response elements [20][21][22][23], including the androgen responsive regions of prostate specific antigen [24], probasin [25,26], and the Slp gene [27].…”

Reduced activity of Candida detachment factors in the saliva of the elderly

“…4 Scheffe's test, Fisher's exact probability test, and Student'st-test were used for statistical analysis. Table 1 shows the results of experiments in which the detachment activity of saliva specimens from the three different age groups was measured by culturing the mycelial form of C. albicans in a culture medium containing 25% saliva (final concentration).…”