Primary structure and chromosomal localization of human and mouse rod photoreceptor cGMP-gated cation channel.

Pittler, Steven J; Lee, Andrea K.; Altherr, Michael R.; Howard, Thad A.; Seldin, Michael F.; Hurwitz, Richard L.; Wasmuth, John J.; Baehr, Wolfgang

doi:10.1016/s0021-9258(18)42689-0

Cited by 100 publications

(7 citation statements)

References 35 publications

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“…The overall a.a. sequence identity between mCNG3 and the next most closely related group of sequences was on the order of 80% (bovine testis/cone channel, 84%; human cone channel, 83%; chicken cone channel, 77%). In contrast, mCNG3 showed only 64% identity to the mouse rod photoreceptor CNG1 α-subunit (Pittler et al, 1991).…”

Section: Molecular Characterization Of a New Cngc α-Subunitmentioning

confidence: 88%

“…The antiserum efficiently labeled HEK cells transfected with EC1 (data not shown), but not untransfected cells or mock-transfected cells. HEK cells transiently transfected with other recombinant CNGCs-human rod CNGC α-and β-subunits (Dhallan et al, 1992;Chen et al, 1993), mouse rod CNGC α-subunit (Pittler et al, 1991), rat olfactory channel α-subunit (Dhallan et al, 1990), bovine rod CNGC β-subunit (Körschen et al, 1995), bovine testis βsubunits (4C, 4D, 4E, Biel et al, 1996)-also showed no cross-reactivity with the mCNG3 antiserum. Therefore, the antiserum does not appear to recognize α-subunits from human (or mouse) rod photoreceptor, rat olfactory channel, or the β-subunits from bovine testis and human and bovine retina.…”

Section: Heterologous Expression Of Cdnasmentioning

confidence: 97%

“…First-strand cDNA template was reverse transcribed from total RNA isolated from mouse (C57BL/6) retinae with oligo (dT) as primer following standard protocols. The initial fragments were amplified using degenerate primers, designed from the highly conserved sequence of the cGMP binding site of the mouse rod photoreceptor cGMP-gated channel [Pittler et al, 1991;ol-1, amino acid (a.a.) residues SPGDYIC; 5′-(A/T)(G/C)(C/T)CC(A/G/T)-GG(A/G/C)GA(C/ T)TA(C/T)AT(A/G/T)TG-3′, sense, and ol-2, a.a. residues KDDLMEA, 5′-AC(C/T)TCCAT antisense]. Two rounds of PCR were performed in a 50-μl reaction volume containing first-strand cDNA as template, 200 pmol of each primer, 200-μM deoxynucleotides, 2.5 U Taq polymerase (Boehringer Mannheim, Indianapolis, IN), and 1× supplied buffer (10 mM Tris-HCl, 1.5 mM MgCl 2 , 50 mM KCl, pH 8.3 (20°C)] and first-strand cDNA as template, or for the second round, 1 μl (1/50th) of the first-round PCR.…”

Section: Cloningmentioning

confidence: 99%

“…The inserts of more than 30 successful recombinants were sequenced (Sequenase 2.0 kit, United States Biochemical/ Amersham Life Sciences, Cleveland, OH). Sequence analysis revealed that the rod photoreceptor CNG channel (Pittler et al, 1991), a cone photoreceptor CNG-like channel (1-21, Bönigk et al, 1993), and one previously unidentified channel were isolated (1-26). A few sequences that did not appear to encode cyclic nucleotide-gated channels were found and disregarded.…”

Section: Cloningmentioning

confidence: 99%

“…To identify additional members of the CNGC family whose expression might be restricted to the retina, mouse retina total RNA was reverse transcribed and the cDNA was amplified by PCR using degenerate oligonucleotide primers, whose sequence was derived from the highly conserved cyclic nucleotide binding domain from the mouse rod photoreceptor CNGC channel (Pittler et al, 1991). The complete 2.8-kb cDNA was obtained by various RT-PCR techniques (degenerate RT-PCR, 5′-and 3′-RACE PCR) and by a screen of a mouse genomic library.…”

Section: Molecular Characterization Of a New Cngc α-Subunitmentioning

confidence: 99%

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Cloning and immunocytochemical localization of a cyclic nucleotide-gated channel ?-subunit to all cone photoreceptors in the mouse retina

et al. 2000

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Cyclic nucleotide-gated channels (CNGC) are ligand-gated ion channels that open and close in response to changes in the intracellular concentration of the second messengers, 3′,5′-cyclic adenosine monophosphate and 3′,5′-cyclic guanosine monophosphate. Most notably, they transduce the chemical signal produced by the absorption of light in photoreceptors into a membrane potential change, which is then transmitted to the ascending visual pathway. CNGCs have also been implicated in the signal transduction of other neurons downstream of the photoreceptors, in particular the ONbipolar cells, as well as in other areas of the central nervous system. We therefore undertook a search for additional cyclic nucleotide-gated channels expressed in the retina. Following a degenerate reverse transcription polymerase chain reaction approach to amplify low-copy number messages, a cDNA encoding a new splice variant of CNGC α-subunit was isolated from mouse retina and classified as mCNG3. An antiserum raised against the carboxy-terminal sequence identified the retinal cell type expressing mCNG3 as cone photoreceptors. Preembedding immunoelectron microscopy demonstrated its membrane localization in the outer segments, consistent with its role in phototransduction. Double-labeling experiments with cone-specific markers indicated that all cone photoreceptors in the murid retina use the same or a highly conserved cyclic nucleotide-gated channel. Therefore, defects in this channel would be predicted to severely impair photopic vision. Indexing termsrat; calcium/calmodulin; phototransduction Cyclic nucleotide-gated channels (CNGC) are nonselective cation channels, constructed out of αand β-subunits in a likely tetrameric configuration, with intracellular ligand-binding domain(s). When expressed in heterologous systems, the α-subunits can form functional ion channels. With the coexpression of the αand β-subunits, the channels acquire additional characteristics that reflect those seen in situ (Kaupp, 1995;Molday, 1996;Wei et al., 1998), for example, the ability to be modulated by calcium (Chen et al., 1993;Körschen et al., 1995). These channels are highly regulated (Zimmerman, 1995) and may be the downstream effectors of nitric oxide-signaling mechanisms (Ahmad et al., 1994;Savchenko et al., 1997).

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Section: Molecular Characterization Of a New Cngc α-Subunitmentioning

confidence: 88%

Section: Heterologous Expression Of Cdnasmentioning

confidence: 97%

Section: Cloningmentioning

confidence: 99%

Section: Cloningmentioning

confidence: 99%

Section: Molecular Characterization Of a New Cngc α-Subunitmentioning

confidence: 99%

See 3 more Smart Citations

Cloning and immunocytochemical localization of a cyclic nucleotide-gated channel ?-subunit to all cone photoreceptors in the mouse retina

et al. 2000

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Photoreceptor Proteins

Cideciyan

2002

Wiley Encyclopedia of Molecular Medicine

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Immunocytochemical staining of AII‐amacrine cells in the rat retina with antibodies against parvalbumin

Wäussle

Grüunert

Röhrenbeck

1993

J of Comparative Neurology

188

178

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The rod dominated rodent retina is the preferred tissue for in vitro studies of mammalian retinal physiology and pharmacology. The rod pathway through the rat retina was investigated, therefore, in order to find out whether its organization follows the mammalian "plan." AII-amacrine cells of the rat retina were injected with Lucifer Yellow to characterize the morphology of this bistratified interneuron of the rod pathway. When sections or whole mounts of the rat retina were stained with antibodies against the calcium binding protein parvalbumin (PV), two different amacrine cell types were labeled: the AII-amacrine cell and a widefield amacrine cell. They occur at a ratio of 12:1. Weak label was also observed in ganglion cells. The density of PV-labeled AII-cells decreases from approximately 7,000 cells/mm2 in upper central retina to 2,000 cells/mm2 in peripheral retina. Their cell bodies form a regular mosaic, and the dendritic arbors of three neighbouring AII-amacrine cells overlap (coverage of 3).

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Primary structure and chromosomal localization of human and mouse rod photoreceptor cGMP-gated cation channel.

Cited by 100 publications

References 35 publications

Cloning and immunocytochemical localization of a cyclic nucleotide-gated channel ?-subunit to all cone photoreceptors in the mouse retina

Cloning and immunocytochemical localization of a cyclic nucleotide-gated channel ?-subunit to all cone photoreceptors in the mouse retina

Photoreceptor Proteins

Immunocytochemical staining of AII‐amacrine cells in the rat retina with antibodies against parvalbumin

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