Primary structure and evolution of rat growth hormone gene.

Barta, Andrea; Richards, Robert I.; Baxter, John D.; Shine, John

doi:10.1073/pnas.78.8.4867

Cited by 249 publications

(105 citation statements)

References 34 publications

(32 reference statements)

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“…An examination of the Pol III promoter sequences revealed that the rGH TR, but not the type 2 DNA, contains a significant A58+G change at an invariant position in the B box promoter region. Surprisingly, we found this change to be conserved in all eleven TR elements sequenced so far (3,7,(9)(10)(11)(12)(13)(14)(15). It could be argued that this "nonpermissive" change of the invariant A58 to a G is responsible for the lack of TR Pol III activity in vitro.…”

Section: Cell-free Transcriptionmentioning

confidence: 83%

“…1). Another single TR is found 300-500 bp beyond the rGH gene polyadenylation signal ( The putative 'identifier" sequence is equivalent to the rGH TR sequences and therefore is a member of the TR family (3,(7)(8)(9)(10)(11)(12)(13)(14)(15).…”

mentioning

confidence: 99%

“…The IB DNA sequence in the Sprague-Dawley rat contains 508 bp of three repeat monomers with a headto-tail array of two repeats that have 86% homology to the CHO type 2 repetitive DNA class (4,7). The third monomer is truncated after the first 73 bp (which are 66% homologous to the type 2 DNA) by an A-rich segment; we have termed this DNA the truncated repeat (TR) family (Fig.…”

mentioning

confidence: 99%

“…The 5.8 kb EcoRI-HindIII rGH gene-containing fragment in prGHeh-5.8 was used (7). The 1250 bp and 1350 bp PstI fragments of prGHeh-5.8 cloned into the PstI site of p8R322 resulted in subclones prGH-1250 (containing I) and prGH-1350 (containing 3'FR) (Fig.…”

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confidence: 99%

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Transcription of two classes of rat growth hormone gene-associated repetitive DNA: differences in activity and effects of tandem repeat structure

et al. 1984

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The rat growth hormone (rGH) gene contains two classes of repetitive DNA arranged as clusters within intron B and the 3' flanking region. The major family is equivalent to the CHO type 2 DNA. The second ("truncated repeat", TR) is a truncated version of the first and occurs in certain neural-specific transcripts and genes ("identifier" elements, ID). Here we report, using the HeLa cell-free transcription assay, that RNA polymerase III (Pol III) efficiently initiates at internal promoters within a tandem array of rGH gene repetitive DNA monomers and results in a novel organization of overlapping Class III transcription units. Transcription competition studies revealed that the rat type 2 structures share Pol III transcription factors with a tRNA gene, a human Alu repeat, and a mutant VAl gene. Also, the rGH type 2 but not the TR DNA efficiently promotes Pol III initiation, yet other TR members, which differ only in flanking DNA, are transcribed. Thus, the rGH gene is strikingly enriched with 10 repetitive DNA monomers; multimeric type 2 elements are actively transcribed; rGH-TR sequences are expressed only as part of larger transcripts promoted by type 2 DNA; and, type 2 DNA uses tRNA gene transcription factors. These studies show that flanking sequences, promoter organization and factor competition may all affect rat repetitive DNA expression. INTRODUCTIONThe major component of mammalian short-length, middle-repetitive DNA is

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Section: Cell-free Transcriptionmentioning

confidence: 83%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Transcription of two classes of rat growth hormone gene-associated repetitive DNA: differences in activity and effects of tandem repeat structure

et al. 1984

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“…Genes compared are from rat growth hormone (rGH), mouse renin genes (Renl and Ren2), human growth hormone (hGH), angiotensinogen and the cu-subunit of mouse nerve growth factor ((uNGF). The positions relative to the principal transcriptional start point have been indicated the rGH (positions -248 to -257 relative to the transcription start point [16]), and in the two mouse renin genes (positions -455 to -464 for Ren 1; -666 to -675 for Ren2 [17]). The second conserved element which is the dodecanucleotide GAAAACAGGTAG is found in the three genes with only one mismatch (the C is replaced by a G in the two renin genes).…”

Section: Discussionmentioning

confidence: 99%

Thyroxine and testosterone transcriptionally regulate renin gene expression in the submaxillary gland of normal and transgenic mice carrying extra copies of the Ren2 gene

Tronik

Rougeon

1988

FEBS Letters

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Expression of the mouse renin genes (Renl and Ren2) in the submaxillary gland of female mice has been analyzed following administration of thyroxine (T4) or dihydrotestosterone (DHT). Both hormones appear to act independently on mRNA accumulation which increases about 5 fold over basal level. In vitro transcription assays in isolated nuclei demonstrate that both hormones act at the transcriptional level. The effects of DHT and T4 were also analyzed in transgenic mice obtained by microinjection of the Ren2 gene. We show that T4 is as efficient as DHT in promoting renin mRNA accumulation in these transgenic animals, in spite of their low basal level of Ren2 mRNA. Structural comparison of the Renl and Ren2 promoters with those of other genes regulated by T4 shows the conservation of two discrete regions.

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Interstrand duplexes in nuclear RNA

Pogo

Miller

1986

BioEssays

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Nuclear intermolecular duplexes appear to be a general feature of nucleated cells. Most of these duplexes are formed between large RNA as well as between large and small RNA molecules. A significant portion of the large molecules belong to a special class of RNA that is restricted to the nucleus and, therefore, not designated for export. These molecules are assembled with proteins and form a structure of a higher order. The possibility that these molecules and a set of small nuclear RNAs are components of a complex machine, “the transportosome”, which functions in nucleocytoplasmic traffic, is discussed.

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Primary structure and evolution of rat growth hormone gene.

Cited by 249 publications

References 34 publications

Transcription of two classes of rat growth hormone gene-associated repetitive DNA: differences in activity and effects of tandem repeat structure

Transcription of two classes of rat growth hormone gene-associated repetitive DNA: differences in activity and effects of tandem repeat structure

Thyroxine and testosterone transcriptionally regulate renin gene expression in the submaxillary gland of normal and transgenic mice carrying extra copies of the Ren2 gene

Interstrand duplexes in nuclear RNA

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