Primary Structure and Molecular Modeling of Mistletoe Lectin I fromViscum album

Eschenburg, Susanna; Krauspenhaar, R.; Mikhailov, A.M.; Stoeva, Stanka; Betzel, Christian; Voelter, Wolfgang

doi:10.1006/bbrc.1998.8760

Cited by 24 publications

(25 citation statements)

References 22 publications

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“…ML I is a highly potent, cytotoxic and immunomodulatory drug, that causes apoptosis in cancer cells [2] and a considerable increase and activation of natural killer cells [3][4][5], initiating an immune cascade.…”

Section: Introductionmentioning

confidence: 99%

Improvement of an enzyme linked lectin assay to determine recombinant mistletoe lectin I

Hussein

Daniels

2007

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 99%

Improvement of an enzyme linked lectin assay to determine recombinant mistletoe lectin I

Hussein

Daniels

2007

Journal of Pharmaceutical and Biomedical Analysis

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“…MLs I, II, and III belong to the ribosome-inactivating protein family of type II-like highly toxic ricin and abrin [3]. Ribosomeinactivating proteins of type II are composed of an Nglycosidase (A chain) and a galactoside-recognizing lectin (B chain) connected by a disulfide bridge [4,5]. VA extracts consist of an aqueous extract of V. album, the European mistletoe [4,6].…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of the Sensitivity of Lymphoblastoid and Transformed Monocytic Cell Lines to the Cytotoxic Effects of <i>Viscum album</i> Extracts of Different Origin

Huyen

Delignat²,

Kazatchkine³

et al. 2003

Chemotherapy

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Viscum album (VA) preparations consist of aqueous extracts of V. album, the European mistletoe. VA extracts contain mistletoe lectins, which are members of the ribosome-inactivating protein type II family. VA preparations have cytotoxic and immunomodulatory properties. Cytotoxicity induced by VA extracts may differ greatly according to the origin of the preparation (host tree, fermented extract) and the cell type. This work was performed to assess the cytotoxicity of various VA preparations, i.e. VA Qu FrF, Qu Spez, M Spez and VA P, in lymphoblastoid and monocytic cell lines. VA Qu FrF, Qu Spez and M Spez induced dose-dependent cell death and inhibition of cell proliferation in lymphoblastoid T cell lines and in transformed monocytic lines. In contrast, the majority of B cell lines tested were resistant to cytotoxicity induced by VA extracts. While VA Qu FrF, Qu Spez and M Spez were potent inducers of cell death, extracts of VA P, derived from mistletoe plants growing on pine trees, failed to induce any cell death in any of the cell lines examined.

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“…For leaves of European mistletoes ( Viscum album ) reduced sulphur is of particular importance, since they contain significant amounts of viscotoxin, a group of cysteine‐rich proteins (Schrader‐Fischer and Apel 1993, Eschenburg et al. 1998, Soler et al.…”

Section: Introductionmentioning

confidence: 99%

“…For leaves of European mistletoes (Viscum album) reduced sulphur is of particular importance, since they contain significant amounts of viscotoxin, a group of cysteine-rich proteins (Schrader-Fischer and Apel 1993, Eschenburg et al 1998, Soler et al 1998, Krauspenhaar et al 1999, Stoeva et al 2001. Viscotoxins are classified as thionins that are toxic against fungi, bacteria, mam- Physiol.…”

Section: Introductionmentioning

confidence: 99%

Seasonal and spatial variation of reduced sulphur compounds in mistletoes (Viscum album) and the xylem sap of its hosts (Populus × euramericana and Abies alba)

Escher¹,

Eiblmeier²,

Hetzger³

et al. 2003

Physiologia Plantarum

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In the present field study with adult trees inhabited by Viscum album, the question was addressed as to whether European mistletoes are able to remove reduced sulphur from the xylem sap of its hosts. For this purpose the reduced sulphur composition and content of the xylem sap of Viscum album and the corresponding hosts Populus × euramericana and Abies alba were analysed. The xylem sap of Viscum was enriched in reduced sulphur compared to the hosts but still reflected the higher reduced sulphur content of Populus compared to Abies. Despite similar xylem sap composition of the hosts with glutathione as the dominating thiol, Viscum on Populus contained predominantly cysteine, Viscum on Abies predominantly glutathione in its xylem sap. These findings suggest selective and different removal of reduced sulphur from these hosts. Still the amount of reduced sulphur removed was too small to result in changes of the concentration of thiols in the xylem sap of the hosts that are statistically significant, probably due to the high variability encountered under field conditions. Despite the differences in the reduced sulphur composition and contents of the xylem sap between Viscum on Populus and Viscum on Abies, total thiol content as well as thiol composition of Viscum leaves on the two hosts were similar throughout the seasons. The seasonal pattern in the thiol composition and contents of Viscum leaves showed high levels in spring and autumn and low levels in summer. The significance of these seasonal changes is discussed.

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Primary Structure and Molecular Modeling of Mistletoe Lectin I fromViscum album

Cited by 24 publications

References 22 publications

Improvement of an enzyme linked lectin assay to determine recombinant mistletoe lectin I

Improvement of an enzyme linked lectin assay to determine recombinant mistletoe lectin I

Comparative Study of the Sensitivity of Lymphoblastoid and Transformed Monocytic Cell Lines to the Cytotoxic Effects of <i>Viscum album</i> Extracts of Different Origin

Seasonal and spatial variation of reduced sulphur compounds in mistletoes (Viscum album) and the xylem sap of its hosts (Populus × euramericana and Abies alba)

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