Primary structure of a cardioactive neuropeptide from the tobacco hawkmoth, <i>Manduca sexta</i>

Cheung, Clement C.; Loi, Poh Kheng; Sylwester, Andy W.; Lee, Terry D.; Tublitz, Nathan J.

doi:10.1016/0014-5793(92)81436-p

Cited by 97 publications

(36 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A likely possibility is that it leads to changes in neuronal excitability, and this hypothesis is consistent with the reports of the release of CCAP at the time of ecdysis (Tublitz and Truman, 1985;Cheung et al, 1992). Such changes could be achieved by the direct gating of ionic channel by cGMP, as is known to occur in photoreceptor and olfactory neurons (Latorre et al, 199 l), or by modifying their kinetic properties by phosphorylation, which accounts, for instance, for the increase in calcium current of some snail neurons (Paupardin-Tritsch et al, 1986).…”

Section: Function Of Cgmp Responsesupporting

confidence: 89%

“…Both pairs of neurons in each abdominal ganglion are peptidergic neurons that are immunoreactive for crustacean cardioactive peptide (CCAP, Fig. 3A,B), a peptide that is present in the abdominal CNS of Manduca (Cheung et al, 1992). One pair of these cells, Cell 27 (Taghert and Truman, 1982), are projection neurons that extend to the next posterior segment and terminate distally on the alary muscle of the heart and proximally in a neurohemal structure, the perivisceral organs (Davis et al, 1993).…”

Section: Identity Of Cgmp-immunoreactive Neuronsmentioning

confidence: 99%

“…Also, at ecdysis there are major adjustments in cardiac activity as blood is redistributed to aid in rupturing the old cuticle and expanding the new one. Indeed, adult ecdysis in Manduca is accompanied by a release of cardioactive peptides (Tublitz and Truman, 1985), one of which is CCAP (Cheung et al, 1992). The cell 27s in the abdomen are the main neurosecretory cells that contain CCAP and would be involved in this release.…”

Section: Role Of Synaptic Interactions In the Eh-induced Increase In mentioning

confidence: 99%

See 2 more Smart Citations

Neuropeptide induction of cyclic GMP increases in the insect CNS: resolution at the level of single identifiable neurons

1994

View full text Add to dashboard Cite

In insects, the neuropeptide eclosion hormone (EH) acts on the CNS to evoke the stereotyped behaviors that cause ecdysis, the shedding of the cuticle at the end of each molt. Concomitantly, EH induces an increase in cyclic GMP (cGMP). Using antibodies against this second messenger, we show that this increase is confined to a network of 50 peptidergic neurons distributed throughout the CNS. Increases appeared 30 min after EH treatment, spread rapidly throughout these neurons, and were extremely long lived. We show that this response is synaptically driven, and does not involve the soluble, nitric oxide (NO)-activated, guanylate cyclase. Stereotyped variations in the duration of the cGMP response among neurons suggest a role in coordinating responses having different latencies and durations.

show abstract

Section: Function Of Cgmp Responsesupporting

confidence: 89%

Section: Identity Of Cgmp-immunoreactive Neuronsmentioning

confidence: 99%

Section: Role Of Synaptic Interactions In the Eh-induced Increase In mentioning

confidence: 99%

See 1 more Smart Citation

Neuropeptide induction of cyclic GMP increases in the insect CNS: resolution at the level of single identifiable neurons

1994

View full text Add to dashboard Cite

show abstract

“…Although there is now an extensive literature documenting the presence of CCAP-like immunoreactivity in a large number of arthropod species (Cheung et al, 1992;Keller, 1992;Davis et al, 1993;Furuya et al, 1993;Lehman et al, 1993;Dircksen, 1994;Trube et al, 1994;Breidbach et al, 1995;Groome and Lehman, 1995;Würden and Homberg, 1995), the physiological actions of CCAP have been considerably less intensively studied. Although CCAP was initially named because of its cardioexcitatory actions on semi-isolated heart preparations of the crab C. maenas (Stangier et al, 1987), not all crustacean hearts seem to be sensitive to CCAP (McGaw et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

“…CCAP was first purified and sequenced from the crab Carcinus maenas (Stangier et al, 1987), and has since been found in a large number of arthropod species (Stangier et al, 1989;Cheung et al, 1992;Furuya et al, 1993;Lehman et al, 1993;Dircksen, 1994).…”

Section: Abstract: Pyloric Rhythm; Crustacean Neuropeptide; Central mentioning

confidence: 99%

Modulation of Oscillator Interactions in the Crab Stomatogastric Ganglion by Crustacean Cardioactive Peptide

et al. 1997

View full text Add to dashboard Cite

The modulation of the pyloric rhythm of the stomatogastric ganglion of the crab, Cancer borealis, by crustacean cardioactive peptide (CCAP) is described. CCAP activated pyloric rhythms in most silent preparations, and altered the phase relationships of pyloric motor neuron firing in all preparations. In CCAP, the pyloric rhythms were characterized by long lateral pyloric (LP) neuron bursts of action potentials. The threshold for CCAP action was ϳ10 Ϫ10 M, with increasing effects at higher CCAP concentrations. The changes in motor pattern evoked by CCAP produced significant changes in LP-innervated muscle movement. These movements were additionally potentiated by CCAP applications to isolated nerve-muscle preparations. Thus, enhanced motor neuron firing and increase of the gain of the neuromuscular junctions are likely to operate coordinately in response to hormonally released CCAP. High CCAP concentrations sometimes resulted in modification of the normal 1:1 alternation between the pyloric dilator (PD) and LP neurons to patterns of 2:1, 3:1, or 4:1 alternation. CCAP seems to activate slow intrinsic oscillations in the LP neuron, as well as enhance faster oscillations in the pacemaker group of PD/ anterior burster (AB) neurons. Simulations of fast and slow oscillators with reciprocal inhibitory coupling suggest mechanisms that could account for the mode switch from 1:1 alternation to multiple PD bursts alternating with one LP neuron burst.

show abstract

Immunocytochemical localization of FLRFamide-, proctolin-, and CCAP-like peptides in the stomatogastric nervous system and neurohemal structures of the crayfish,Cherax destructor

1999

View full text Add to dashboard Cite

To compare the stomatogastric nervous system of the crayfish Cherax destructor with those of other decapod species, the distribution of FLRF (Phe‐Leu‐Arg‐Phe) amide‐, proctolin‐ and crustacean cardioactive peptide (CCAP)‐like immunoreactivities was studied in the stomatogastric nervous system and in neurosecretory structures by using wholemount immunocytochemical techniques and confocal microscopy. In addition, the number of cells in the stomatogastric ganglion (19–24) and axon profiles in the stomatogastric nerve (157–165) were counted. FLRFamide‐like immunoreactivity was present within numerous cell bodies and neuropil of the commissural ganglia, in the neuropil of the stomatogastric ganglion, and in one cell body of the esophageal ganglion. FLRFamide‐like immunoreactivity was also found in two cell bodies at the junction of the stomatogastric nerve with the superior esophageal nerve and in two cell bodies in the inferior ventricular nerve. Proctolin‐like immunoreactivity was present in numerous cell bodies and neuropil of the paired commissural ganglia and in the neuropil of the stomatogastric ganglion. CCAP‐like immunoreactivity was found in the neuropil and in one to four cell bodies in the commissural ganglia. Both proctolin‐ and CCAP‐immunoreactive varicosities occurred on the surface of the circumesophageal connectives and on the postesophageal commissure, indicating a neurohemal source within the stomatogastric nervous system, which was verified by electron microscopy. The pericardial organs showed FLRFamide‐, proctolin‐, and CCAP‐like immunoreactivity. This staining pattern suggests that FLRFamide‐like and proctolin‐like peptides are used as neurohormones and as neuromodulators in the stomatogastric nervous system of the crayfish C. destructor, whereas CCAP‐like peptides may only affect the stomatogastric ganglion as a neurohormone. J. Comp. Neurol. 414:511–532, 1999. © 1999 Wiley‐Liss, Inc.

show abstract

Primary structure of a cardioactive neuropeptide from the tobacco hawkmoth, Manduca sexta

Cited by 97 publications

References 13 publications

Neuropeptide induction of cyclic GMP increases in the insect CNS: resolution at the level of single identifiable neurons

Neuropeptide induction of cyclic GMP increases in the insect CNS: resolution at the level of single identifiable neurons

Modulation of Oscillator Interactions in the Crab Stomatogastric Ganglion by Crustacean Cardioactive Peptide

Immunocytochemical localization of FLRFamide-, proctolin-, and CCAP-like peptides in the stomatogastric nervous system and neurohemal structures of the crayfish,Cherax destructor

Contact Info

Product

Resources

About