Primary structure of human carcinoembryonic antigen (CEA) deduced from cDNA sequence

Detection of colonic cells in peripheral blood of colorectal cancer patients by means of reverse transcriptase and polymerase chain reaction

“…and its expression is maintained in almost all colorectal carcinomas (Shively and Beatty, 1985). Its genomic organization (Schrewe et al, 1990) and cDNA sequence (Oikawa et al, 1987) are known. In addition, this target has been used in the specific detection of CEA-expressing tumour cells in bone marrow aspirates (Gerhard et al, 1994) and, recently, in the identification of circulating neoplastic cells in patients with colorectal liver metastasis (Jonas et al, 1996).…”

Intra- and interspecies analyses of the carcinoembryonic antigen (CEA) gene family reveal independent evolution in primates and rodents

“…This t e n d e n c y is m o s t obvious for the m o r e distantly related genes h s C G M 2 and h s C G M 3 (see Fig. 5 (Oikawa et al 1987c, and unpublished data), BGP1 ), hsCGM1-4 ( Thompson et al 1989), PSOG ; Watanabe and Chou 1988a), rnCGMI-5 (Kodelja et al 1989). …”

“…Immunological characterization and isolation of various cross-reacting antigens already indicated the existence ofa CEA gene family (reviewed in Thompson and Zimmermann 1988). These preliminary data could be confirmed at the protein (Engvall et al 1978;Kessler et al 1978;Paxton et al 1987) and DNA levels (Oikawa et al 1987c; Thompson et al 1987;Neumaier et al 1988). Southern analyses indicated the existence of 9-11 genes in humans (Thompson et al 1987).…”

“…Southern analyses indicated the existence of 9-11 genes in humans (Thompson et al 1987). Sequencing ofgenomic and cDNA clones has revealed the complete primary protein structure of CEA (Beauchemin et al 1987;Oikawa et al 1987c;Zimmermann et al 1987), NCA (Oikawa et al 1987b;Thompson et al 1987;Neumaier et al 1988;Tawaragi et al 1988), and a pregnancy-specific /31 glycoprotein (PS/~G) (Watanabe and Chou 1988a). The highly conserved domains shared by each are a 34-amino-acid leader peptide, a 108-110-amino-acid N-terminal domain, a 178-180-amino-acid repeating unit of which three copies are present in CEA, whereas only one and a half can be found in PSBG and one in NCA, and a 26-aminoacid carboxyl region (CEA) that is 2 amino acids shorter in NCA and degenerate in PSI3G.…”

Carcinoembryonic antigen-immunoglobulin Fc fusion protein (CEA-Fc) for identification and activation of anti-CEA immunoglobulin-T-cell receptor-modified T cells, representative of a new class of Ig fusion proteins

“…CEA consists of five regions: an IgV-like N-terminal region of 108 residues, three highly similar Ig I-like repetitive regions (Repeats I, II, and III, each consisting of a pair of subdomains A1 and B1, A2 and B2 and A3 and B3, respectively) of 178 residues each, and a highly hydrophobic C-terminal region of 26 residues that is removed during processing to allow attachment of the GPI anchor. 17,19,20 In the plan to make the Fc fusion protein, we considered that the size of product would be unwieldy if we used intact CEA to make a molecule of the form CEA 2 -Fc (ca. 450 kDa).…”