Primary structure of the trpC gene from Aspergillus nidulans

Mullaney, Edward J.; Hamer, John E.; Roberti, Kellee A.; Yelton, M. Melanie; Timberlake, William E.

doi:10.1007/bf00327506

Cited by 203 publications

(101 citation statements)

References 43 publications

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“…These sites were used to introduce the PCR product into pAN01 that had been cut with the same enzymes. Vector pAN01is a derivative of pAN2-4, which contains the gpd promoter and trpC terminator (Punt et al 1990;Mullaney et al 1985). The gpd promoter is replaced by the glaA promoter of 822 bp in pAN01.…”

Section: Complementation Of the Dflug Strainmentioning

confidence: 99%

FluG affects secretion in colonies of Aspergillus niger

Wang

Krijgsheld

Hulsman

et al. 2014

Antonie van Leeuwenhoek

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Colonies of Aspergillus niger are characterized by zonal heterogeneity in growth, sporulation, gene expression and secretion. For instance, the glucoamylase gene glaA is more highly expressed at the periphery of colonies when compared to the center. As a consequence, its encoded protein GlaA is mainly secreted at the outer part of the colony. Here, multiple copies of amyR were introduced in A. niger. Most transformants over-expressing this regulatory gene of amylolytic genes still displayed heterogeneous glaA expression and GlaA secretion. However, heterogeneity was abolished in transformant UU-A001.13 by expressing glaA and secreting GlaA throughout the mycelium. Sequencing the genome of UU-A001.13 revealed that transformation had been accompanied by deletion of part of the fluG gene and disrupting its 3 0 end by integration of a transformation vector. Inactivation of fluG in the wild-type background of A. niger also resulted in breakdown of starch under the whole colony. Asexual development of the DfluG strain was not affected, unlike what was previously shown in Aspergillus nidulans. Genes encoding proteins with a signal sequence for secretion, including part of the amylolytic genes, were more often downregulated in the central zone of maltose-grown DfluG colonies and upregulated in the intermediate part and periphery when compared to the wild-type. Together, these data indicate that FluG of A. niger is a repressor of secretion.Keywords Fungus Á Conidiogenesis Á Asexual development Á Aspergillus fluG Á Secretion Á Heterogeneity Electronic supplementary material The online version of this article

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Section: Complementation Of the Dflug Strainmentioning

confidence: 99%

FluG affects secretion in colonies of Aspergillus niger

Wang

Krijgsheld

Hulsman

et al. 2014

Antonie van Leeuwenhoek

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“…radicislycopersici and F. oxysporum strain Fo47, the ecfp gene was introduced between the Aspergillus nidulans gpdA promoter (Punt et al 1988) and the trpC terminator (Mullaney et al 1985) sequences as follows. Plasmid pGDPGFP (Lagopodi et al 2002), which contains the sgfp gene between the gpdA promoter and the trpC terminator, was digested with NcoI and HindIII in order to isolate the sgfp gene (Fig.…”

Section: Cloning Of the Ecfp And Eyfp In Pgpdgfp And Its Expression Imentioning

confidence: 99%

Visualization of Interactions Between a Pathogenic and a Beneficial Fusarium Strain During Biocontrol of Tomato Foot and Root Rot

Bolwerk¹,

Lagopodi²,

Lugtenberg³

et al. 2005

MPMI

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The soilborne fungus Fusarium oxysporum f. sp. radicislycopersici causes tomato foot and root rot (TFRR), which can be controlled by the addition of the nonpathogenic fungus F. oxysporum Fo47 to the soil. To improve our understanding of the interactions between the two Fusarium strains on tomato roots during biocontrol, the fungi were labeled using different autofluorescent proteins as markers and subsequently visualized using confocal laser scanning microscopy. The results were as follows. i) An at least 50-fold excess of Fo47over F. oxysporum f. sp. radicis-lycopersici was required to obtain control of TFRR. ii) When seedlings were planted in sand infested with spores of a single fungus, Fo47 hyphae attached to the root earlier than those of F. oxysporum f. sp. radicis-lycopersici. iii) Subsequent root colonization by F. oxysporum f. sp. radicis-lycopersici was faster and to a larger extent than that by Fo47. iv) Under disease-controlling conditions, colonization of tomato roots by the pathogenic fungus was significantly reduced. v) When the inoculum concentration of Fo47 was increased, root colonization by the pathogen was arrested at the stage of initial attachment to the root. vi) The percentage of spores of Fo47 that germinates in tomato root exudate in vitro is higher than that of the pathogen F. oxysporum f. sp. radicis-lycopersici. Based on these results, the mechanisms by which Fo47 controls TFRR are discussed in terms of i) rate of spore germination and competition for nutrients before the two fungi reach the rhizoplane; ii) competition for initial sites of attachment, intercellular junctions, and nutrients on the tomato root surface; and iii) inducing systemic resistance.

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“…1A. structed a plasmid pTREB, which carried bar for selection and the promoter and terminator regions of Aspergillus nidulans trpC for ectopic expression (44). This plasmid has a multicloning site between the trpC promoter and terminator for SmaI, EcoRI, EcoRV, and ClaI.…”

Section: P72hmentioning

confidence: 99%

A Point Mutation in Nucleoside Diphosphate Kinase Results in a Deficient Light Response for Perithecial Polarity in Neurospora crassa

Ogura

Yoshida

Yabe

et al. 2001

Journal of Biological Chemistry

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In Neurospora crassa, the phosphorylation of nucleoside diphosphate kinase (NDK)-1 is rapidly enhanced after blue light irradiation. We have investigated the function of NDK-1 in the blue light signal transduction pathway. A mutant called psp (phosphorylation of small protein) shows undetectable phosphorylation of NDK-1 and is defective in light-responsive regulation of perithecial polarity. Sequencing analysis of ndk-1 cDNA by reverse transcription-polymerase chain reaction revealed that proline 72 of ndk-1 was replaced with histidine in psp. The mutation ndk-1 P72H resulted in accumulation of normal levels of mRNA and of about 25% of NDK-1 P72H protein compared with that of wild type as determined by Western blot analysis. The ectopic expression of cDNA and introduction of genomic DNA of wild type ndk-1 in psp (ndk-1 P72H ) suppressed the reduction in accumulation and phosphorylation of NDK-1 and the light-insensitive phenotype. These findings demonstrated that the phenotype of psp was caused by the ndk-1 P72H mutation. Biochemical analysis using recombinant NDK-1 and NDK-1 P72H indicated that the P72H substitution in NDK-1 was responsible for the decrease in phosphotransfer activities, 5% of autophosphorylation activity, and 2% of V max for protein kinase activity phosphorylating myelin basic protein, compared with those of wild type NDK-1, respectively.

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Primary structure of the trpC gene from Aspergillus nidulans

Cited by 203 publications

References 43 publications

FluG affects secretion in colonies of Aspergillus niger

FluG affects secretion in colonies of Aspergillus niger

Visualization of Interactions Between a Pathogenic and a Beneficial Fusarium Strain During Biocontrol of Tomato Foot and Root Rot

A Point Mutation in Nucleoside Diphosphate Kinase Results in a Deficient Light Response for Perithecial Polarity in Neurospora crassa

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