Primary structure of toxin IV of Leiurus quinquestriatus quinquestriatus

Abstract

We report the sequence of toxin IV of Leiurus quinquestriatus quinquestriatus, one of the live toxins lethal for the mouse purified from the venom of this scorpion. Automatic sequencing of the S-carboxymethylated protein and derived peptides obtained by action of the Staphylococcus aureus protease allowed us to work out the complete sequence of 65 residues. The toxin which was found to be blocked at the C-terminal end has an extra residue at the N-terminus as compared with other toxins from Asian and African scorpions. Moreover, toxin IV must be classified as an a-scorpion toxin since it was shown to displace competitively rz51-toxin II of Androctonus australis Hector ( lzsI-AaH II) from its binding site on rat brain synaptosomes. Nevertheless, it cannot be classified in one of the groups of scorpion toxins previously defined on the level of their antigenic properties. Again the classifications of scorpion toxins based on sequence homologies or antigenic properties support one another. Scorpion toxin Amino acid sequence Automatic sequencing Scorpion toxin classiJication

Pharmacology of the Na v 1.1 domain IV voltage sensor reveals coupling between inactivation gating processes

“…To test whether shifts in SSI are a common feature of VSDIVbinding toxins, we examined the effects of LqqIV, a VSDIV-binding α-scorpion toxin (22,23) using this same protocol. Similar to Hm1a, LqqIV shifted the SSI curve to more positive potentials (V 1/2 = 67 ± 3 mV) (Fig.…”

“…Hm1a was purified from Heteroscodra maculata venom as described previously (10). LqqIV was provided by Martin-Eauclaire and Pierre Bougis, Aix-Marseille Université (22). ICA-121431 was purchased from (Table S1).…”

Makatoxin I, a Novel Toxin Isolated from the Venom of the Scorpion Buthus martensi Karsch, Exhibits Nitrergic Actions

“…(7,8), Lqq IV and Lqq III from L. quinquestriatus quinquestriatus (9,10), Lqh ␣IT from L. quinquestriatus hebraeus (IT-insect toxin) (11), Bom III from B. occitanus mardochei (12), and Css II from C. suffusus suffusus (13).…”

“…It is 81% similar to that of Bot II (7) and 78% similar to that of Bot I (8), both of which were isolated from North African scorpion B. occitanus tunetanus venom. From 55-77% similarity was found when compared with other toxins of Lqq IV (9) and Lqq III (10) (13) from Centruroides suffusus suffusus, which showed only 25% similarity (Fig. 4B).…”

Notes on gestation periods and litter size in the arenicolous buthid scorpion Leiurus quinquestriatus (Ehrenberg, 1828) (Scorpiones: Buthidae)

“…Leiurus quinquestriatus venom is the most toxic reported among scorpion species (LD50: 0.25 mg/kg), capable of provoking both dysfunction and failure of cardiovascular and respiratory systems (13)(14)(15)(16)(17)(18)(19)(20)(21). Neurotoxic venoms are particularly toxic to infants and young children, due to their weight-dependent effects (14).…”