Introduction:Multimodal training (MMT) is important for healthy ageing. However, few middle-aged (MAA) and older adults (OA) engage in MMT. This study aimed to (1) explore MAA and OA reflections, impact and future recommendations following 'Prime Time of Life' (PTOL) -a 12-week online multimodal physical activity and health education programme; and (2) examine if participants implemented and sustained lifestyle changes during and after the programme.Methods: A qualitative study was conducted with purposeful sampling to explore the experiences of MAA and OA who completed PTOL. Six semi-structured focus groups and five interviews were conducted using online communication technology, audiorecorded, and transcribed verbatim. Transcripts were analysed using thematic analysis.Results: Twenty-one male (n = 7,65.9 ± 6.6 years) and female (n = 14,65 ± 7.6 years) participants completed the study. Accessibility, health benefits, suitability, social influence, and accountability were identified as key facilitators. Poor awareness, social influence, accessibility, and poor self-efficacy were perceived as potential barriers. Participants primarily (90.5%, n = 19) made lifestyle changes during the PTOL programme, and 61.9% (n = 13) maintained at least one lifestyle change seven months after completing the programme.Discussion: Although lack of social interaction between participants was identified as a barrier, PTOL was a well-accepted method to promote healthy ageing for MAA and OA. Given there was a poor awareness of other MMT programmes and health benefits prior to PTOL there is a need for increased awareness. It is important to MAA and OA that they have convenient access to MMT programmes that are suitable for all fitness and functional abilities and provide technical support.