Primer análisis filogenético de Ehrlichia canis en perros y garrapatas de México. Estudio preliminar

Abstract

Objective. Phylogenetic characterization of Ehrlichia canis in dogs naturally infected and ticks, diagnosed by PCR and sequencing of 16SrRNA gene; compare different isolates found in American countries. Materials and methods. Were collected Blood samples from 139 dogs with suggestive clinical manifestations of this disease and they were infested with ticks; part of 16SrRNA gene was sequenced and aligned, with 17 sequences reported in American countries. Two phylogenetic trees were constructed using the Maximum likelihood method, and Maximum parsimony. Results. They were positive to E. canis 25/139 (18.0%) dogs and 29/139 (20.9%) ticks. The clinical manifestations presented were fever, fatigue, depression and vomiting. Rhipicephalus sanguineus Dermacentor variabilis and Haemaphysalis leporis-palustris ticks were positive for E. canis. Phylogenetic analysis showed that the sequences of dogs and ticks in Mexico form a third group diverging of sequences from South America and USA. Conclusions. This is the first phylogenetic analysis of E. canis in Mexico. There are differences in the sequences of Mexico with those reported in South America and USA. This research lays the foundation for further study of genetic variability.

Prevalence of Borrelia burgdorferi, Anaplasma spp., Ehrlichia spp. and Dirofilaria immitis in Canadian dogs, 2008 to 2015: a repeat cross-sectional study

“…This is presumed due to their respective tick vector presence in the country, i.e. Dermacentor variabilis ( E. canis ) [28, 29] and I. scapularis , I. cookei (EMLA) [30, 31]. However, D. variabilis has only been shown to be experimentally infected with E. canis , so its role in natural infection and exposure in dogs is unclear [28].…”

Detección molecular de Ehrlichia canis, Anaplasma platys y Rickettsia rickettsii en caninos domésticos del municipio de Cajeme, Sonora, México

“…Microorganisms of the order Rickettsiales have zoonotic conditions called Rickettsiosis, Ehrlichiosis and Anaplasmosis that are due to several pathogens of veterinary importance that have gained ground not only in our region but also worldwide , this is mainly due to its vector Rhipicephalus sanguineus, being the most commonly reported tick species and with the greatest geographic distribution (Sosa et al, 2016;Cabezas-Cruz et al, 2019). These pathogens are increasing due to the activity of human beings that has generated radical changes in the environment, favoring vector-borne diseases (Suthers, 2004) and increasing their prevalence in late spring and summer.…”

Molecular Detection and Characterization of Ehrlichia canis Isolates from Three Geographic Regions in Mexico: A Retrospective Study

“…Moreover, the 16S rRNA gene has previously been used as a target and the sequences obtained have been applied in the genetic characterization of E. canis strains in dogs from Peru [ 25 ], Greece [ 26 ], and, more recently, Turkey [ 27 ]. Previous E. canis studies in Mexico used 16S rRNA gene and trp36 as target genes for phylogenetic analysis [ 16 , 17 , 28 , 29 , 30 ].…”