Primer Note: A New Set of Highly Polymorphic Nuclear Microsatellite Markers for Nothofagus nervosa and Related South American Species

Abstract

Nothofagus is the main component of southern South American temperate forests. Overexploitation in the past has led to the loss of 40% of the original distribution range. Genetic diversity as well as biological processes shaping the distribution of the genetic variation (e.g. gene flow) constitutes basic knowledge for the implementation of conservation measures and for the definition of Evolutionary Significant Units. Nuclear microsatellites are the marker of choice for gene flow and fine-scale genetic structure studies. We enlarged a previous set of microsatellites (SSRs) for South American Nothofagus species, with special concern to Nothofagus nervosa (Phil.) Dim. et Mil. Five new SSRs are presented with allele numbers up to 12 in a single population. The primers transferred well to five related species (N. obliqua (Mirb.) Oerst, N. glauca (Phil.) Krasser, N. dombeyi (Mirb.) Oerst , N. pumilio (Poepp et Endl.) Krasser and N. antarctica (G. Forster) Oerst, with allele numbers up to 11. The high level of polymorphism promises a sufficient power for gene flow and parentage analyses.

Out in the cold: genetic variation of Nothofagus pumilio (Nothofagaceae) provides evidence for latitudinally distinct evolutionary histories in austral South America

“…2004; Jones et al. 2004; Marchelli et al. 2008) their transferability to N. pumilio on analyses of 500 individuals from 10 populations have yielded a comparable number of alleles to those found using isozymes (M. Arbetman and P. Mathiasen, Universidad Nacional del Comahue).…”

“…Isozyme polymorphisms were previously used in all species within subgenus Nothofagus including N. pumilio (Premoli 1997(Premoli , 2003Premoli & Kitzberger 2005;Steinke et al 2008). Although microsatellites for Nothofagus were developed for some species within subgenus Lophozonia (Azpilicueta et al 2004;Jones et al 2004;Marchelli et al 2008) their transferability to N. pumilio on analyses of 500 individuals from 10 populations have yielded a comparable number of alleles to those found using isozymes (M. Arbetman and P. Mathiasen, Universidad Nacional del Comahue).…”

Population genetic structure, genetic diversity, and natural history of the South American species of Nothofagus subgenus Lophozonia (Nothofagaceae) inferred from nuclear microsatellite data

“…), and five for N. alpina (Marchelli et al. ). Polymerase chain reactions (PCR) followed Jones et al.…”

The effect of volcanism on postglacial migration and seed dispersal. A case study in southern South America

“…Three simple sequence repeats (SSRs) were scored: NnBIO11, NgBIO14 (Azpilicueta et al 2004), and NnBIO111 (Marchelli et al 2007). PCR was performed in a total volume of 20 μl containing about 10 ng of total DNA, 1,5 mM MgCl 2 , 100 μM each dNTP, 0.2 μM each primer, and 0.8 U Taq polymerase (Invitrogen) with the respective 10× PCR buffer, using a Biometra Uno Thermo Block.…”