Priming for and induction of anti-poliovirus neutralizing antibodies by synthetic peptides

Emini, Emilio A.; Jameson, Bradford A.; Wimmer, Eckard

doi:10.1038/304699a0

Cited by 237 publications

(138 citation statements)

References 31 publications

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“…2). This sequence, conserved in all FCV isolates so far examined, is an area that according to computer analysis (Emini et al, 1985) contains a hydrophilic region of high surface probability surrounded by hydrophobic regions with low surface probability. These conserved amino acids might possibly be involved with virus attachment with the hydrophilic portion surrounded by hydrophobic areas providing a 'canyon' for virus attachment similar to the situation of rhinovirus binding (Rossmann et al, 1985).…”

Section: S ! O C F Cmentioning

confidence: 99%

Analysis of feline calicivirus capsid protein genes: identification of variable antigenic determinant regions of the protein

Seal¹,

Ridpath

Mengeling³

1993

Journal of General Virology

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Section: S ! O C F Cmentioning

confidence: 99%

Analysis of feline calicivirus capsid protein genes: identification of variable antigenic determinant regions of the protein

Seal¹,

Ridpath

Mengeling³

1993

Journal of General Virology

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“…After virus challenge, titres of gp70 antibodies increased drastically only in the pepl-KLH group. Similar priming by immunization with synthetic peptides has been reported after immunization against poliovirus or foot-and-mouth disease virus using synthetic vaccines (Emini et al, 1983;Francis et al, 1985).…”

Section: Multi-peptide Vaccines But Not Single-peptide Vaccines Incrementioning

confidence: 78%

Synthetic Vaccines against Friend Murine Leukaemia Virus-induced Erythroleukaemia: in vivo and in vitro Studies with Synthetic Oligopeptides and Sequence-specific Antisera

Bayer¹,

Hunsmann²

1987

Journal of General Virology

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SUMMARYBiological activities of antisera against synthetic oligopeptides were examined. The peptide antisera were directed against amino acids 6 to 12 (pepl), 124 to 131 (pep2), 256 to 262 (pep3), 283 to 290 (pep4) and 434 to 441 (pep5) of the viral envelope glycoprotein (gp70). Peptide-specific antisera did not neutralize viral infectivity. However, antibodies to pep4 and pep5, which bound to the hydrophobic part of gp70, mediated the complement-dependent lysis of Friend murine leukaemia virus (FLV)-infected cells. STU mice were immunized against FLV-induced erythroleukaemia with synthetic oligopeptides. Vaccines containing only one of these peptides (single-peptide vaccines) as well as multi-peptide vaccines containing pepl, -4, -5 or pepl, -2, -3, -4, -5 were used. Whereas the immunizations with single-peptide vaccines did not protect the immunized mice from FLV-induced erythroleukaemia, multi-peptide vaccines enhanced the survival rate and incubation period after FLV challenge. These results revealed that immunological reactions distinct from neutralizing antibodies can be evoked by immunization with synthetic peptides and can confer limited protection against FLV-induced erythroleukaemia.

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“…The cDNA fragment introduced into vaccinia virus has the complete coding sequence of VP4, VP2 and VP3, and the N-terminal 57 amino acids of VP1. This N-terminal portion of VP1 would, therefore, also incorporate the epitope described by Emini et al (1985) and, as shown by the immunoblot experiments, the VP1 peptide antibody to this epitope binds to the expressed HAV polyprotein.…”

Section: Discussionmentioning

confidence: 99%

A recombinant vaccinia virus expressing hepatitis A virus structural polypeptides: characterization and demonstration of protective immunogenicity

Karayiannis¹,

O’Rourke²,

McGarvey³

et al. 1991

Journal of General Virology

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A recombinant vaccinia virus containing most of the P 1 region of hepatitis A virus (HAV) was constructed. Cell lysates of cultures infected with the virus contained HAV proteins detectable by radioimmunoassay. Western blot analysis revealed the presence of a single protein of Mr 60K to 62K, bearing epitopes from structural polypeptides VP4, -3 and -2, and the N terminus of VP1. The size of the protein suggests that at least some of the vaccinia virus thymidine kinase is also expressed. Inoculation of tamarin monkeys with the recombinant virus resulted in the development of a specific anti-HAV immune response which was protective against challenge with a virulent strain of HAV. Recombinant viruses expressing the above region of HAV or the proteins expressed by such viruses may be useful in the development of a vaccine suitable for use in man.

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Priming for and induction of anti-poliovirus neutralizing antibodies by synthetic peptides

Cited by 237 publications

References 31 publications

Analysis of feline calicivirus capsid protein genes: identification of variable antigenic determinant regions of the protein

Analysis of feline calicivirus capsid protein genes: identification of variable antigenic determinant regions of the protein

Synthetic Vaccines against Friend Murine Leukaemia Virus-induced Erythroleukaemia: in vivo and in vitro Studies with Synthetic Oligopeptides and Sequence-specific Antisera

A recombinant vaccinia virus expressing hepatitis A virus structural polypeptides: characterization and demonstration of protective immunogenicity

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