Primitive Defence: The MiAMP1 Antimicrobial Peptide Family

Manners, John M.

doi:10.1007/s11105-008-0083-y

Cited by 21 publications

(18 citation statements)

References 31 publications

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“…2003; Ekramoddoullah, Liu & Zamani 2006). The expression of these genes is activated during infection by pathogens and their expression products secreted into the apoplast (Manners 2009). A new family within AMP called β ‐barrelins includes all these novel plant AMPs (McManus et al .…”

Section: Introductionmentioning

confidence: 99%

A maritime pine antimicrobial peptide involved in ammonium nutrition

Canales

Ávila

Cánovas³

2011

Plant Cell & Environment

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A large family of small cysteine-rich antimicrobial peptides (AMPs) is involved in the innate defence of plants against pathogens. Recently, it has been shown that AMPs may also play important roles in plant growth and development. In previous work, we have identified a gene of the AMP b-barrelin family that was differentially regulated in the roots of maritime pine (Pinus pinaster Ait.) in response to changes in ammonium nutrition. Here, we present the molecular characterization of two AMP genes, PpAMP1 and PpAMP2, showing different molecular structure and physicochemical properties. PpAMP1 and PpAMP2 displayed different expression patterns in maritime pine seedlings and adult trees. Furthermore, our expression analyses indicate that PpAMP1 is the major form of AMP in the tree, and its relative abundance is regulated by ammonium availability. In contrast, PpAMP2 is expressed at much lower levels and it is not regulated by ammonium. To gain new insights into the function of PpAMP1, we overexpressed the recombinant protein in Escherichia coli and demonstrated that PpAMP1 strongly inhibited yeast growth, indicating that it exhibits antimicrobial activity. We have also found that PpAMP1 alters ammonium uptake, suggesting that it is involved in the regulation of ammonium ion flux into pine roots.

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Section: Introductionmentioning

confidence: 99%

A maritime pine antimicrobial peptide involved in ammonium nutrition

Canales

Ávila

Cánovas³

2011

Plant Cell & Environment

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“…In vitro antifungal activity against several phytopathogens, as well as yeast, has also been demonstrated for homologous peptides from three different pine species (Sooriyaarachchi et al 2011;Canales et al 2011;Zamany et al 2011). The b-barrelin precursor proteins have a signal peptide and in gymnosperms expression is induced by fungal attack, supporting an in vivo defensive role (Manners 2009;Sooriyaarachchi et al 2011).…”

Section: B-barrelinsmentioning

confidence: 89%

“…The b-barrelin or MiAMP1 family is present in gymnosperms, angiosperms, and spikemosses, although they seem to be missing in several species with sequenced genomes (Manners 2009). It was discovered when a peptide purified from macadamia nuts, called MiAMP1, showed antimicrobial activity against several fungi and a gram-positive bacteria, but not gram-negative bacteria or mammalian cells (Marcus et al 1997).…”

Section: B-barrelinsmentioning

confidence: 99%

New Antimicrobial Agents of Plant Origin

Sampedro

Valdivia

2013

Antimicrobial Compounds

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Plants are constantly under attack by microbial pathogens. As part of their defensive arsenal, they use antimicrobial peptides such as thionins, defensins, lipid transfer proteins, hevein-like peptides, knottins, cyclotides, b-barrelins, and others. In addition, they produce a diversity of antimicrobial metabolites. Those where the evidence for a role in plant defense is stronger include benzoxazinoids, camalexin, and glucosinolates among the alkaloids; flavonoids and stilbenes among the phenylpropanoids; and also terpenoids such as saponins. Our understanding of these plant antimicrobial agents has increased significantly in recent years with new information on their distribution, synthesis, regulation, in vivo function, and mechanism of action. Plant antimicrobial agents have a large potential for biotechnological applications. Engineered plants with increased disease resistance have been achieved using almost every family of antimicrobial peptides. There have been also been successes in using metabolic engineering to increase the production of antimicrobial compounds, as in the case of stilbenes and glucosinolates. Commercial applications using both approaches are likely to appear soon. The use of plant antimicrobials in human medicine is probably further in the future, although there are promising antifungal agents like defensin peptides, and saponins. With more than a quarter million species and a particularly diverse specialized metabolism, the richness of plant antimicrobials has barely been explored. Plant Antimicrobial DefensesWild plants are quite successful at keeping bacterial and fungal pathogens at bay. In addition to physical barriers, they have an immune system capable of detecting and responding to the presence of pathogens. The first layer of defense is based on

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“…Other unclassified AFPs (not listed in Table ) include peptides isolated from the Japanese bamboo, Phyllostachys pubescens ( Pp‐AMP ) (Fujimura, Ideguchi, Minami, Watanabe, & Tadera, ), and the basic 38 to 44 residues ToAMPs , identified from Taraxacum officinale flowers (Astafieva et al., ). TnAFP1 , an interesting 1.2‐kDa peptide isolated from water chestnut, or MiAMP1 , from the nut kernel of Macadamia integrifolia (Manners, ) belong to the class of AFPs. MiAMP1 is a 76‐amino acids basic peptide, which has a particular Greek key motif that has also been found in yeast killer toxin.…”

Section: Plant Sources Of Afpsmentioning

confidence: 99%

Natural Antifungal Peptides/Proteins as Model for Novel Food Preservatives

Thery

Lynch

Arendt

2019

Comp Rev Food Sci Food Safe

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A large range of ingredients for food and food products are subject to fungal contamination, which is a major cause of destruction of crops, exposure of animals and humans to invasive mycotoxins, and food spoilage. The resistance of fungal species to common preservation methods highlights the necessity of new ways to increase the shelf life of raw material for food and food products. Antimicrobial peptides and proteins (AMPs) are essential members of the immune system of most living organisms. Due to their broad range of activity and their stability to commonly used food processes, they represent promising alternatives to traditional preservatives. However, despite the growing number of reports of potential food applications of these AMPs, the number of approved peptides is low. Poor solubility, toxicity, and a time‐consuming extraction are hurdles that limit their application in food products. Thanks to a deep understanding of the key determinants of their activity, the development of optimized synthetic peptides has reduced these drawbacks. This review presents natural and synthetic antifungal peptides/proteins (AFPs), effective against food‐related fungi, with particular emphasis on AFPs from plant sources. The design of novel antifungal peptides via key elements of antifungal activity is also reviewed. The potential applications of natural and synthetic AFPs as novel antifungal food preservatives are finally discussed.

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Primitive Defence: The MiAMP1 Antimicrobial Peptide Family

Cited by 21 publications

References 31 publications

A maritime pine antimicrobial peptide involved in ammonium nutrition

A maritime pine antimicrobial peptide involved in ammonium nutrition

New Antimicrobial Agents of Plant Origin

Natural Antifungal Peptides/Proteins as Model for Novel Food Preservatives

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