2015
DOI: 10.1039/c4sc03258f
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Principal factors that determine the extension of detection range in molecular beacon aptamer/conjugated polyelectrolyte bioassays

Abstract: A new bioassay strategy based on the molecular beacon aptamer/conjugated polyelectrolyte demonstrates a fine-tuning of the detection range and limit of detection for weakly-binding targets.

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Cited by 11 publications
(5 citation statements)
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“…Fluorescence resonance energy transfer (FRET) can be utilized for multicolor imaging because a single donor excitation can result in fluorescence emission of various acceptor dyes. FRET involves nonradiative energy transfer between donor and acceptor molecules via dipole–dipole coupling and has been widely used for fluorescent and ratiometric sensing. Because the FRET efficiency is inversely proportional to the donor–acceptor intermolecular distance raised to the sixth power, Pdots that encage the fluorophores within the nanosized particles can be an appropriate platform to locate donor and acceptor dyes in close proximity. Furthermore, the photoluminescence (PL) of red- and near-infrared (NIR)-emitting probes can be enhanced via the antenna effect of CPs, which can be advantageous for biological imaging owing to the deep tissue penetration and negligible autofluorescence.…”
Section: Introductionmentioning
confidence: 99%
“…Fluorescence resonance energy transfer (FRET) can be utilized for multicolor imaging because a single donor excitation can result in fluorescence emission of various acceptor dyes. FRET involves nonradiative energy transfer between donor and acceptor molecules via dipole–dipole coupling and has been widely used for fluorescent and ratiometric sensing. Because the FRET efficiency is inversely proportional to the donor–acceptor intermolecular distance raised to the sixth power, Pdots that encage the fluorophores within the nanosized particles can be an appropriate platform to locate donor and acceptor dyes in close proximity. Furthermore, the photoluminescence (PL) of red- and near-infrared (NIR)-emitting probes can be enhanced via the antenna effect of CPs, which can be advantageous for biological imaging owing to the deep tissue penetration and negligible autofluorescence.…”
Section: Introductionmentioning
confidence: 99%
“…In previous works, the adenosine-induced aptamer conformational switch and DNA duplex dissociation have been used for design of ATP-responsive sensor, the disassembly of nanoparticles, and controlled drug release. [48][49][50] Here, to investigate whether the surface property of hydrogel could be programmed with ATP molecules, we labelled the cDNA with a uorescent dye, uorescein isothiocyanate (FITC) (Fig. 2a).…”
Section: Resultsmentioning
confidence: 99%
“…In our strategy, hydrogel was chosen as the supporting substrate due to its excellent abilities to simulate natural extracellular matrix. The ATP aptamer (Apt-DNA) [48][49][50] and its complementary DNA (cDNA) were rst conjugated onto the hydrogel. They acted as intelligent mediators to immobilize the cell-adhesive RGD ligand.…”
Section: Introductionmentioning
confidence: 99%
“…A MBA containing the ATP‐specific aptamer sequence in its loop part (GGGG AGTA TTGC GGAG GA) with a fluorophore (6‐FAM) and quencher (DABCYL) in both termini was selected as the ATP probe (Figure ) . The ATP‐specific MBA probe preferentially takes a hairpin conformation via hydrogen bonding interactions in its stem part.…”
Section: Resultsmentioning
confidence: 99%