Principles and Application of Confocal Microscopy to Understand Symbiotic Fungi

Dabral, S. N.; Bhola, Diksha; Attri, Manpreet Kaur; Nath, Manoj; Prasad, Ram; Singh, Surender; Varma, Ajit

doi:10.1007/978-3-319-68867-1_19

Cited by 1 publication

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“…Acridine orange can also serve as a counter stain by binding to the soil humic material, and this technical offshoot has been exploited to observe fungal (Pythium aphanidermatum) zoospores on cucumber roots (Sørensen et al 2009). Specific cell stains are often used with confocal laser scanning microscopy (CLSM) and florescence microscopy and have been used extensively to study the structural and spatial composition of microbial communities in environmental samples (Dabral et al 2017). Strain specific fluorescent-antibody staining has been used to study bacterial root colonization by Azospirillum in the rhizosphere of wheat (Scholter et al 1993).…”

Section: Methods Of Studying Rhizosphere Microfloramentioning

confidence: 99%

Ecosystem Diversity as a Function of Plant and Soil-Microbe Interactions

Dey

Bhattacharyya²,

Bhattacharyya

2020

Plant Microbiome Paradigm

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Microbial diversity in soil is overwhelming, and so is their interaction with roots of higher plants. Rhizosphere is the area of the soil around the roots of higher plants where amazingly intense cross talk occurs with soil microbes, and they form a continuous network in a particular ecosystem. It is now well established that underground microbial diversity determines community composition of plants and of the ecosystem in an area. This is possible due to the diverse form of plant and soilmicrobe interaction occurring in the rhizosphere. In this review it has been our endeavour to elucidate ecosystem diversity as a function of plant and soil-microbe interactions. We have focussed primarily on soil microbial diversity determining plant community composition. However, literature concerning ecosystem diversity is scarce. To our understanding this could be a potential area of ecological research in years to come. IntroductionSince beginning of study concerning diversity in ecosystem, ecologists have theorized and/or hypothesized the process as a function of anthropogenic activities (Nelson et al. 2006), ecosystem size (Blakely and Didham 2010), invasive species and infectious diseases (Crowl et al. 2008). It also includes various other aboveground abiotic factors like climate, age, environmental harshness, disturbance, environmental heterogeneity, as well as biotic interactions (Tilman and Pacala 1993). However, attention to the belowground drivers (soil microbes) of ecosystem

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Section: Methods Of Studying Rhizosphere Microfloramentioning

confidence: 99%