Principles and Applications of Force Spectroscopy Using Atomic Force Microscopy

Kim, Youngkyu; Kim, Woong; Park, Chong-Yun

doi:10.1002/bkcs.11022

Cited by 15 publications

(17 citation statements)

References 152 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Information obtained by SMFS is significant for understanding of the relations between molecular structure and functions of various substances . SMFS is used for determination of very small forces in a range of pN, provides information about sample topography, their mechanical properties and is useful for study the thermodynamics and kinetics of molecular interactions . SMFS is excellent tool for direct measurements of intermolecular bonds, for obtaining information on infrequent molecular states and on mechanical properties of molecules that are important for protein functions .…”

Section: Resultsmentioning

confidence: 99%

DNA Aptamers in the Detection of Leukemia Cells by the Thickness Shear Mode Acoustics Method

et al. 2019

View full text Add to dashboard Cite

By using the thickness shear mode acoustics method (TSM) and single-molecule force spectroscopy (SMFS) we studied the interactions between DNA aptamers (sgc8c) specific to the protein tyrosine kinase 7 (PTK7), which is localized in the membranes of leukemia lymphoblastics (MOLT-4), and lymphocyte (Jurkat) cell lines, as well with PTK7-negative U266 myeloid leukemia cells. The TSM method allowed the development of a highly sensitive, label-free biosensor for the detection leukemia cells with a limit of detection of (195 � 20) cells/mL. SMFS approved the high selectivity of the sgc8c aptamers to the PTK7 receptors at the cell surface and allowed determining the binding probability of the aptamers to the PTK7 receptors at different cell lines.

show abstract

Section: Resultsmentioning

confidence: 99%

DNA Aptamers in the Detection of Leukemia Cells by the Thickness Shear Mode Acoustics Method

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The intra- and intermolecular interactions of the polymer are obtained directly through the stretching of the polymer bridge by the AFM tip in different surrounding environments. A detailed review of AFM in terms of the fundamentals of force spectroscopy, sample preparation, data analysis and applications, and its future, is presented by Park and his coworkers [ 23 ]. Gaub et al found that the elongation of the polymer chains was governed by the distortion of the bond angles at higher forces through SMFS.…”

Section: Introductionmentioning

confidence: 99%

Single-Chain Mechanical Properties of Gelatin: A Single-Molecule Study

Zhang

Guo

et al. 2022

Polymers

View full text Add to dashboard Cite

Gelatin is an important natural biological resource with a wide range of applications in the pharmaceutical, industrial and food industries. We investigated the single-chain behaviors of gelatin by atomic force microscopy (AFM)-based single-molecule force spectroscopy (SMFS), and found that gelatin exists as long chains by fitting with the M-FJC model. By comparing the single-chain elasticity in a nonpolar organic solvent (nonane) and DI water, it was surprising to find that there was almost no difference in the single-chain elasticity of gelatin in nonane and DI water. Considering the specificity of gelatin solubility and the solvent size effect of nonane molecules, when a single gelatin chain is pulled into loose nonane, dehydration does not occur due to strong binding water interactions. Gelatin chains can only interact with water molecules at high temperatures; therefore, no further interaction of single gelatin chains with water molecules occurred at the experimental temperature. This eventually led to almost no difference in the single-chain F–E curves under the two conditions. It is expected that our study will enable the deep exploration of the interaction between water molecules and gelatin and provide a theoretical basis and experimental foundation for the design of gelatin-based materials with more functionalities.

show abstract

“…Herein, we report a direct approach to detect KRAS G12D mutation in ctDNA samples extracted from patients’ plasma with very low mutant allele frequencies (0.006–0.2%) at high sensitivity/specificity (near 100%) by force–distance (F-D) curve-based AFM. − The remarkable specificity of MutS protein, which binds to only the mismatched DNA duplexes in lieu of fully matched ones, was utilized to sense only the mutated genes. A specifically designed LNA/DNA chimeric blocker was placed into the ctDNA sample solution prior to the denaturation step to make the desired target sequence accessible to the capture probe.…”

mentioning

confidence: 99%

Direct Detection of Low Abundance Genes of Single Point Mutation

et al. 2021

Self Cite

View full text Add to dashboard Cite

Cell-free DNA (cfDNA) analysis, specifically circulating tumor DNA (ctDNA) analysis, provides enormous opportunities for noninvasive early assessment of cancers. To date, PCR-based methods have led this field. However, the limited sensitivity/specificity of PCR-based methods necessitates the search for new methods. Here, we describe a direct approach to detect KRAS G12D mutated genes in clinical ctDNA samples with the utmost LOD and sensitivity/specificity. In this study, MutS protein was immobilized on the tip of an atomic force microscope (AFM), and the protein sensed the mismatched sites of the duplex formed between the capture probe on the surface and mutated DNA. A noteworthy LOD (3 copies, 0.006% allele frequency) was achieved, along with superb sensitivity/specificity (100%/100%). These observations demonstrate that force-based AFM, in combination with the protein found in nature and properly designed capture probes/blockers, represents an exciting new avenue for ctDNA analysis.

show abstract

Principles and Applications of Force Spectroscopy Using Atomic Force Microscopy

Cited by 15 publications

References 152 publications

DNA Aptamers in the Detection of Leukemia Cells by the Thickness Shear Mode Acoustics Method

DNA Aptamers in the Detection of Leukemia Cells by the Thickness Shear Mode Acoustics Method

Single-Chain Mechanical Properties of Gelatin: A Single-Molecule Study

Direct Detection of Low Abundance Genes of Single Point Mutation

Contact Info

Product

Resources

About