Principles, Applications and Future Aspects of Cold‐Adapted PGPR

Khan, Mahejibin; Goel, Reeta

doi:10.1002/9783527621989.ch10

Cited by 3 publications

(2 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Antarctic continent is considered to be an attractive source of novel bacteria for biotechnological use in diverse fields, such as in the production of antimicrobial and antitumor compounds for biomedicine and the production of ice-binding proteins for the food industry [ 16 , 17 , 18 ]. In this context, cold-tolerant bacteria have also been shown to contribute to the survival and adaptation of plants at low temperatures, and their potential use as inoculants in agriculture to promote plant growth has also been suggested [ 19 , 20 ]. Recently, bacteria isolated from the phyllosphere of the Antarctic vascular plant ( Deschampsia antarctica ) and selected based on their ice-recrystallization-inhibition (IRI) activity were shown to harbor diverse genetic traits associated with plant growth promotion, such as genes involved in nutrient uptake (NH₄⁺ assimilation and N 2 fixation) and the generation of bioactive metabolites (indole acetic acid and ACC deaminase) and antimicrobial compounds (hydrogen cyanide and pyoverdine) [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Cold-Tolerant Hyper-ACC-Degrading Bacteria from the Rhizosphere, Endosphere, and Phyllosphere of Antarctic Vascular Plants

et al. 2020

View full text Add to dashboard Cite

1-Aminociclopropane-1-carboxylate (ACC)-degrading bacteria having been widely studied for their use in alleviating abiotic stresses in plants. In the present study, we isolated and characterized ACC-degrading bacteria from the rhizosphere, phyllosphere, and endosphere of the Antarctic vascular plants Deschampsia antarctica and Colobanthus quitensis. One hundred and eighty of the 578 isolates (31%) were able to grow on minimal medium containing ACC, with 101 isolates (23, 37, and 41 endosphere-, phyllosphere- and rhizosphere-associated isolates, respectively) identified as being genetically unique by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Subsequently, freeze/thaw treatments and ice-recrystallization-inhibition (IRI) activity assays were performed, the results of which revealed that 77 (13%) of cold-tolerant isolates exhibited putative ACC deaminase activity. Significant (p ≤ 0.05) differences in IRI activity were also observed between the studied plant niches. Surprisingly, all the cold-tolerant isolates showed ACC deaminase activity, independent of the plant niches, with 12 isolates showing the highest ACC deaminase activities of 13.21–39.56 mmol α KB mg protein−1 h−1. These isolates were categorized as ‘cold-tolerant hyper-ACC-degrading bacteria’, and identified as members of Pseudomonas, Serratia, and Staphylococcus genera. The results revealed the occurrence of cold-tolerant hyper-ACC-degrading bacteria in diverse plant niches of Antarctic vascular plants, that could be investigated as novel microbial inoculants to alleviate abiotic stresses in plants.

show abstract

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Cold-Tolerant Hyper-ACC-Degrading Bacteria from the Rhizosphere, Endosphere, and Phyllosphere of Antarctic Vascular Plants

et al. 2020

View full text Add to dashboard Cite

show abstract

“…These microorganisms comprise extremophiles, e.g. Polaromonas (Margesin et al, 2012; Gawor et al, 2018) or Hydrogenophaga (Khan and Goel, 2008) and organisms modulating plant stress response, such as Novosphingobium (Etesami and Beattie, 2018; Vives-Peris et al, 2018). In our study, only Stenotrophomonas and Bacillus species were more frequent in roots of sugar beet than of sea beet.…”

Section: Discussionmentioning

confidence: 99%

Effect of osmoprotectants on the survival of bacterial endophytes in lyophilized beet roots

Szymańska

Sikora

Hrynkiewicz

et al. 2019

Preprint

View full text Add to dashboard Cite

The increase of human population and associated increasing demand for agricultural products lead to soil over-exploitation. Biofertilizers based on lyophilized plant material containing living plant growth-promoting microorganisms (PGPM) could be an alternative to conventional fertilizers that fits into sustainable agricultural technologies ideas. We aimed to: (i) assess the diversity of endophytic bacteria in beet roots and (ii) determine the influence of osmoprotectants addition during lyophilization on bacterial density, viability, salt tolerance. Microbiome diversity was assessed based on 16S rRNA amplicons sequencing, bacterial density and salt tolerance was evaluated in cultures, while bacterial viability was calculated by using fluorescence microscopy and flow cytometry. Here we show that plant genotype shapes its endophytic microbiome diversity and determines physicochemical rhizosphere soil properties. Sea beet endophytic microbiome, consisting of genera characteristic for extreme environments, is more diverse and salt resistant than its crop relative. Supplementing osmoprotectants during root tissue lyophilization exerts a positive effect on bacterial community salt stress tolerance, viability and density. Trehalose improves these parameters more effectively than ectoine, moreover its use is economically advantageous.

show abstract

Choosing source of microorganisms and processing technology for next generation beet bioinoculant

Szymańska

Sikora

Hrynkiewicz

et al. 2021

Sci Rep

View full text Add to dashboard Cite

The increase of human population and associated increasing demand for agricultural products lead to soil over-exploitation. Biofertilizers based on lyophilized plant material containing living plant growth-promoting microorganisms (PGPM) could be an alternative to conventional fertilizers that fits into sustainable agricultural technologies ideas. We aimed to: (1) assess the diversity of endophytic bacteria in sugar and sea beet roots and (2) determine the influence of osmoprotectants (trehalose and ectoine) addition during lyophilization on bacterial density, viability and salt tolerance. Microbiome diversity was assessed based on 16S rRNA amplicons sequencing, bacterial density and salt tolerance was evaluated in cultures, while bacterial viability was calculated by using fluorescence microscopy and flow cytometry. Here we show that plant genotype shapes its endophytic microbiome diversity and determines rhizosphere soil properties. Sea beet endophytic microbiome, consisting of genera characteristic for extreme environments, is more diverse and salt resistant than its crop relative. Supplementing osmoprotectants during root tissue lyophilization exerts a positive effect on bacterial community salt stress tolerance, viability and density. Trehalose improves the above-mentioned parameters more effectively than ectoine, moreover its use is economically advantageous, thus it may be used to formulate improved biofertilizers.

show abstract

Principles, Applications and Future Aspects of Cold‐Adapted PGPR

Cited by 3 publications

References 84 publications

Isolation and Characterization of Cold-Tolerant Hyper-ACC-Degrading Bacteria from the Rhizosphere, Endosphere, and Phyllosphere of Antarctic Vascular Plants

Isolation and Characterization of Cold-Tolerant Hyper-ACC-Degrading Bacteria from the Rhizosphere, Endosphere, and Phyllosphere of Antarctic Vascular Plants

Effect of osmoprotectants on the survival of bacterial endophytes in lyophilized beet roots

Choosing source of microorganisms and processing technology for next generation beet bioinoculant

Contact Info

Product

Resources

About