1987
DOI: 10.1016/s0737-0806(87)80025-4
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Principles of cryopreservation and a review of cryopreservation of stallion spermatozoa

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Cited by 336 publications
(196 citation statements)
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“…Zootec., v.65, n.1, p.6-12, 2013 significant decrease in the percentage of motile cells and vigor throughout the cooling time in both seasons. The cooling process can cause irreversible damage to sperm due to heat shock, which can be minimized by the slow cooling of diluted semen in a ratio of 0.05ºC/min to 4°C (Pickett and Amann, 1993) and by adding extenders containing phospholipids, such as milk or egg yolk (Amann and Pickett, 1987;Den Daas, 1992). However, the dilution of goat semen in extenders containing egg yolk can be deleterious to sperm cells.…”
Section: Discussionmentioning
confidence: 99%
“…Zootec., v.65, n.1, p.6-12, 2013 significant decrease in the percentage of motile cells and vigor throughout the cooling time in both seasons. The cooling process can cause irreversible damage to sperm due to heat shock, which can be minimized by the slow cooling of diluted semen in a ratio of 0.05ºC/min to 4°C (Pickett and Amann, 1993) and by adding extenders containing phospholipids, such as milk or egg yolk (Amann and Pickett, 1987;Den Daas, 1992). However, the dilution of goat semen in extenders containing egg yolk can be deleterious to sperm cells.…”
Section: Discussionmentioning
confidence: 99%
“…1,2 Assim, visando minimizar o efeito deletério do cálcio durante o processo de congelação, a partir da década de 1970 alguns pesquisadores passaram a empregar o etileno-diamino-tetra-acetatodissódico (EDTA) em meios diluidores para a congelação de sêmen eqüino. 3 Sua principal função seria quelar o cálcio no meio extracelular, diminuindo seu influxo para o meio intracelular 4 , inibindo, assim, o efeito deletério do cálcio sobre os espermatozóides 2 .…”
Section: Introductionunclassified
“…Portando, danos nesta estrutura, que é a mais afetada pelo choque frio, podem levar à perda da homeostase, comprometimento da sobrevivência espermática no trato genital da fêmea, perda da sua capacidade fertilizante e posterior morte celular (AMANN; PICKETT, 1987 Segundo Yanagimachi (1994), a capacitação espermática é um pré-requisito para a reação acrossomal na maioria dos mamíferos, que consiste na fusão entre a membrana externa do acrossomo e a porção da membrana plasmática que o envolve, levando à liberação das enzimas acrossomais.…”
Section: Integridade Da Membrana Plasmáticaunclassified