Principles of Uv Degradation

Wypych, George

doi:10.1016/b978-1-927885-61-1.50008-6

Cited by 12 publications

(16 citation statements)

References 100 publications

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“…S16). Generalized Beer–Lambert laws for complex mixtures were adopted for calculating decreasing amounts of substrates (Wypych, 2015). In addition, GC–MS confirmed that purified NaAER1 proteins with NADPH, a biological reducing agent, can convert benzalacetone to BA (Fig.…”

Section: Resultsmentioning

confidence: 99%

Single‐cell RNA‐sequencing of Nicotiana attenuata corolla cells reveals the biosynthetic pathway of a floral scent

et al. 2022

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High-throughput single-cell RNA sequencing (scRNA-Seq) identifies distinct cell populations based on cell-to-cell heterogeneity in gene expression. By examining the distribution of the density of gene expression profiles, we can observe the metabolic features of each cell population.Here, we employ the scRNA-Seq technique to reveal the entire biosynthetic pathway of a flower volatile.The corolla of the wild tobacco Nicotiana attenuata emits a bouquet of scents that are composed mainly of benzylacetone (BA). Protoplasts from the N. attenuata corolla limbs and throat cups were isolated at three different time points, and the transcript levels of > 16 000 genes were analyzed in 3756 single cells.We performed unsupervised clustering analysis to determine which cell clusters were involved in BA biosynthesis. The biosynthetic pathway of BA was uncovered by analyzing gene co-expression in scRNA-Seq datasets and by silencing candidate genes in the corolla.In conclusion, the high-resolution spatiotemporal atlas of gene expression provided by scRNA-Seq reveals the molecular features underlying cell-type-specific metabolism in a plant.

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Section: Resultsmentioning

confidence: 99%

Single‐cell RNA‐sequencing of Nicotiana attenuata corolla cells reveals the biosynthetic pathway of a floral scent

et al. 2022

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“…By adapting the schematic diagram of the stabilization mechanism using a phosphite antioxidant reported by Voigt and Todesco [ 43 ], Figure 11 presents the proposed mechanism of UV protection by PDA. In more detail, the mechanism of free radical scavenging by PDA is as follows: a polymer (RH), when exposed to radiation (UV light), will form radicals (R•).…”

Section: Resultsmentioning

confidence: 99%

Two-Step Dopamine-to-Polydopamine Modification of Polyethersulfone Ultrafiltration Membrane for Enhancing Anti-Fouling and Ultraviolet Resistant Properties

et al. 2020

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Polydopamine has been widely used as an additive to enhance membrane fouling resistance. This study reports the effects of two-step dopamine-to-polydopamine modification on the permeation, antifouling, and potential anti-UV properties of polyethersulfone (PES)-based ultrafiltration membranes. The modification was performed through a two-step mechanism: adding the dopamine additive followed by immersion into Tris-HCl solution to allow polymerization of dopamine into polydopamine (PDA). The results reveal that the step of treatment, the concentration of dopamine in the first step, and the duration of dipping in the Tris solution in the second step affect the properties of the resulting membranes. Higher dopamine loadings improve the pure water flux (PWF) by more than threefold (15 vs. 50 L/m2·h). The extended dipping period in the Tris alkaline buffer leads to an overgrowth of the PDA layer that partly covers the surface pores which lowers the PWF. The presence of dopamine or polydopamine enhances the hydrophilicity due to the enrichment of hydrophilic catechol moieties which leads to better anti-fouling. Moreover, the polydopamine film also improves the membrane resistance to UV irradiation by minimizing photodegradation’s occurrence.

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“…However, these criteria are already a requirement for chiral HPLC methods that use UV spectroscopy in their detectors. UV-CD cannot be applied to UV-sensitive molecules that become modified or degrade under UV light. − Other chiroptical techniques like vibrational circular dichroism (VCD) or Raman optical activity can be a good alternative to UV-CD, as they present more pronounced spectra that arise from the vibration modes of the bonds, and thus are not limited by chemical degradation and absorption requirements. Both techniques also produce spectra, and therefore offer big possibilities in terms of data analysis with multivariate analysis to build quantification methods for chiral molecules.…”

Section: Discussionmentioning

confidence: 99%

Multicomponent Chiral Quantification with Ultraviolet Circular Dichroism Spectroscopy: Ternary and Quaternary Phase Diagrams of Levetiracetam

et al. 2022

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Chiral molecules are challenging for the pharmaceutical industry because although physical properties of the enantiomers are the same in achiral systems, they exhibit different effects in chiral systems, such as the human body. The separation of enantiomers is desired but complex, as enantiomers crystallize most often as racemic compounds. A technique to enable the chiral separation of racemic compounds is to create an asymmetry in the thermodynamic system by generating chiral cocrystal(s) using a chiral coformer and using the solubility differences to enable separation through crystallization from solution. However, such quaternary systems are complex and require analytical methods to quantify different chiral molecules in solution. Here, we develop a new chiral quantification method using ultraviolet-circular dichroism spectroscopy and multivariate partial least squares calibration models, to build multicomponent chiral phase diagrams. Working on the quaternary system of (R)- and (S)-2-(2-oxopyrrolidin-1-yl)butanamide enantiomers with (S)-mandelic acid in acetonitrile, we measure accurately the full quaternary phase diagram for the first time. By understanding the phase stabilities of the racemic compound and the enantiospecific cocrystal, the chiral resolution of levetiracetam could be designed due to a large asymmetry in overall solubility between both sides of the racemic composition. This new method offers improvements for chiral molecule quantification in complex multicomponent chiral systems and can be applied to other chiral spectroscopy techniques.

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Principles of Uv Degradation

Cited by 12 publications

References 100 publications

Single‐cell RNA‐sequencing of Nicotiana attenuata corolla cells reveals the biosynthetic pathway of a floral scent

Single‐cell RNA‐sequencing of Nicotiana attenuata corolla cells reveals the biosynthetic pathway of a floral scent

Two-Step Dopamine-to-Polydopamine Modification of Polyethersulfone Ultrafiltration Membrane for Enhancing Anti-Fouling and Ultraviolet Resistant Properties

Multicomponent Chiral Quantification with Ultraviolet Circular Dichroism Spectroscopy: Ternary and Quaternary Phase Diagrams of Levetiracetam

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