Printability study of hydrogel solution extrusion in nanoclay yield-stress bath during printing-then-gelation biofabrication

Jin, Yifei; Chai, Wenxuan; Huang, Yong

doi:10.1016/j.msec.2017.05.144

Cited by 131 publications

(178 citation statements)

References 29 publications

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“…As reported in a previous study [26], v path can affect the filament diameter significantly during material extrusion. The effect of v path on the filament diameter is pronounced during filament extrusion when a filament is first extruded out of the nozzle with an initial diameter equivalent to D N and a speed of v ink , which is a function of the pressure, path speed, nozzle geometries, and rheological properties as reported [26].…”

Section: Estimation Of Optimal Standoff Distancesupporting

confidence: 67%

“…The effect of v path on the filament diameter is pronounced during filament extrusion when a filament is first extruded out of the nozzle with an initial diameter equivalent to D N and a speed of v ink , which is a function of the pressure, path speed, nozzle geometries, and rheological properties as reported [26]. Then the extruded filament moves at a speed equivalent to v path with an equivalent filament diameter (D E ).…”

Section: Estimation Of Optimal Standoff Distancementioning

confidence: 85%

“…As a result, the NIPAAm solution has poor extrudability and cannot be used alone for extrusion-based printing applications. Figure 3b-1 and b-2 shows the extrusion process of the Laponite nanoclay suspension, a Bingham plastic material with the unique yield-stress property [12,13,25,26] a nozzle, the Laponite nanoclay suspension in the nozzle is sheared and behaves as a shear thinning fluid material. If the applied stress is higher than its shear yield stress, it behaves like liquid; otherwise, it behaves like solid.…”

Section: Effects Of Nanoclay On Extrudabilitymentioning

confidence: 99%

“…Generally, v ink can be estimated by [26], where η 0 is the zero-shear-rate viscosity of ink materials, L is the nozzle length, and R N is the nozzle radius. Considering the geometries of both homemade nozzle used in the extrudability investigation and dispensing nozzle used in the standoff distance investigation, v ink herein can be approximated as 2.13 mm/s based on the measured axial velocity (1.8 mm/s) during the extrudability investigation.…”

Section: Estimation Of Optimal Standoff Distancementioning

confidence: 99%

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Study of extrudability and standoff distance effect during nanoclay-enabled direct printing

2018

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Nanoclay-enabled self-supporting printing has been emerging as a promising filament-based extrusion fabrication approach for different biomedical and engineering applications including tissue engineering. With the addition of nanoclay powders, liquid build materials may exhibit solid-like behavior upon extrusion and can be directly printed in air into complex three-dimensional structures. The objective of this study is to investigate the effect of nanoclay on the extrudability of N -isopropylacrylamide (NIPAAm) and the effect of standoff distance on the print quality during nanoclay-enabled direct printing. It is found that the addition of nanoclay can significantly improve the NIPAAm extrudability and effectively eliminate die swelling in material extrusion. In addition, with the increase of standoff distance, deposited filaments change from over-deposited to well-defined to stretched to broken, the filament width decreases, and the print fidelity deteriorates. A mathematical model is further proposed to determine the optimal standoff distance to achieve better print fidelity during nanoclay-enabled direct printing. Based on the extrudability and standoff distance knowledge from this study, NIPAAm-Laponite nanoclay and NIPAAm-Laponite nanoclay-graphene oxide nanocomposite hydrogel precursors are successfully printed into a three-layered one-dimensional responsive pattern, demonstrating the good extrudability and print quality during nanoclay-enabled printing under optimal printing conditions.

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Section: Estimation Of Optimal Standoff Distancesupporting

confidence: 67%

Section: Estimation Of Optimal Standoff Distancementioning

confidence: 85%

Section: Effects Of Nanoclay On Extrudabilitymentioning

confidence: 99%

Section: Estimation Of Optimal Standoff Distancementioning

confidence: 99%

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Study of extrudability and standoff distance effect during nanoclay-enabled direct printing

2018

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“…To balance high cell livability and good morphology of spheroids, sodium alginate solutions were formulated as a concentration of 2% (w/v) to guarantee adequate stiffness. Because hydrogel with low concentration may cause structure deformation, and contrary, high concentration will lead to poor cell viability and printability . Besides, after spheroids preliminary gelified with 2% (w/v) CaCl 2 solution, they will be transferred into 4% (w/v) CaCl 2 solution for further solidification less than 5 min.…”

mentioning

confidence: 99%

Airflow‐Assisted 3D Bioprinting of Human Heterogeneous Microspheroidal Organoids with Microfluidic Nozzle

Zhao

Chen

Shao

et al. 2018

Small

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Hydrogel microspheroids are widely used in tissue engineering, such as injection therapy and 3D cell culture, and among which, heterogeneous microspheroids are drawing much attention as a promising tool to carry multiple cell types in separated phases. However, it is still a big challenge to fabricate heterogeneous microspheroids that can reconstruct built-up tissues' microarchitecture with excellent resolution and spatial organization in limited sizes. Here, a novel airflow-assisted 3D bioprinting method is reported, which can print versatile spiral microarchitectures inside the microspheroids, permitting one-step bioprinting of fascinating hydrogel structures, such as the spherical helix, rose, and saddle. A microfluidic nozzle is developed to improve the capability of intricate cell encapsulation with heterotypic contact. Complex structures, such as a rose, Tai chi pattern, and single cell line can be easily printed in spheroids. The theoretical model during printing is established and process parameters are systematically investigated. As a demonstration, a human multicellular organoid of spirally vascularized ossification is reconstructed with this method, which shows that it is a powerful tool to build mini tissues on microspheroids.

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Large Scale Tissues Bioprinting

2021

Cell Assembly With 3D Bioprinting

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Printability study of hydrogel solution extrusion in nanoclay yield-stress bath during printing-then-gelation biofabrication

Cited by 131 publications

References 29 publications

Study of extrudability and standoff distance effect during nanoclay-enabled direct printing

Study of extrudability and standoff distance effect during nanoclay-enabled direct printing

Airflow‐Assisted 3D Bioprinting of Human Heterogeneous Microspheroidal Organoids with Microfluidic Nozzle

Large Scale Tissues Bioprinting

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