Printed optics: phantoms for quantitative deep tissue fluorescence imaging

Bentz, Brian Z.; Bowen, Anna G.; Lin, Dergan; Ysselstein, Daniel; Huston, Davin H.; Rochet, Jean‐Christophe; Webb, Kevin J.

doi:10.1364/ol.41.005230

Cited by 19 publications

(15 citation statements)

References 18 publications

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“…Our lab has pioneered the fabrication of 3D-printed biophotonic phantoms [31], developing models with both idealized and biomimetic vascular geometries for evaluation of hyperspectral and fluorescence imaging systems [31][32][33]. Other groups have also shown success in developing 3D-printed phantoms for different biophotonic applications [34,35]. Channel array phantoms not only enable the use of CO-oximetry for referencing of blood samples but provide a matrix material that is stable during measurements and relatively easy to fabricate, handle, and measure.…”

Section: Introductionmentioning

confidence: 99%

Cerebral oximetry performance testing with a 3D-printed vascular array phantom

Afshari

Ghassemi

Lin

et al. 2019

Biomed. Opt. Express

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Cerebral oximetry based on near-infrared spectroscopy represents a unique noninvasive tool for real-time surgical monitoring, yet studies have shown a significant discrepancy in accuracy among commercial systems. Towards the establishment of a standardized method for performance testing, we have studied a solid phantom approachbased on a 3D-printed cerebrovascular module (CVM) incorporating an array of 148 cylindrical channels-that has several advantages over liquid phantoms. Development and characterization of a CVM prototype are described, including high-resolution imaging and spectrophotometry measurements. The CVM was filled with whole bovine blood tuned over an oxygen saturation range of 30-90% and molded-silicone layers simulating extracerebral tissues were used to evaluate penetration depth. Saturation measurement accuracy was assessed in two commercially-available clinical cerebral oximeters. For one oximeter, both neonatal and pediatric sensors showed a high degree of precision, whereas accuracy was strongly dependent on saturation level and extracerebral geometry. The second oximeter showed worse precision, yet greater robustness to variations in extracerebral layers. These results indicate that 3D-printed channel array phantoms represent a promising new approach for standardized testing of clinical oximeters.

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Section: Introductionmentioning

confidence: 99%

Cerebral oximetry performance testing with a 3D-printed vascular array phantom

Afshari

Ghassemi

Lin

et al. 2019

Biomed. Opt. Express

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“…Despite this need, no imaging targets for FGS have been widely adopted or made commercially available. This is partly due to challenges related to manufacturing scalability, application-specific optical spectra, and long-term stability of the imaging targets [6][7][8][9][10][11] . 3D printing of fluorescent materials with tunable optical properties has been developed in this study as a tool to address these challenges.…”

mentioning

confidence: 99%

“…To address photostability issues linked to organic fluorophores, quantum dots 16,18,19 and doped-polymers 20 have been proposed, both of which suffer from the limited tuning of the application-specific spectra and biological relevance. In recent years, 3D printed (3DP) biomedical optics phantoms have shown the ability to mimic anatomical structures and streamline the manufacturing process, with the main drawback of providing only limited tuning of absorption and scattering properties [6][7][8][9][10] . Despite these advances in 3D printing, no comprehensive method for simultaneous incorporation of fluorophores and fine-tuning of absorption and scattering properties has been presented.…”

mentioning

confidence: 99%

3D printing fluorescent material with tunable optical properties

Ruiz

Garg

Streeter

et al. 2021

Sci Rep

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The 3D printing of fluorescent materials could help develop, validate, and translate imaging technologies, including systems for fluorescence-guided surgery. Despite advances in 3D printing techniques for optical targets, no comprehensive method has been demonstrated for the simultaneous incorporation of fluorophores and fine-tuning of absorption and scattering properties. Here, we introduce a photopolymer-based 3D printing method for manufacturing fluorescent material with tunable optical properties. The results demonstrate the ability to 3D print various individual fluorophores at reasonably high fluorescence yields, including IR-125, quantum dots, methylene blue, and rhodamine 590. Furthermore, tuning of the absorption and reduced scattering coefficients is demonstrated within the relevant mamalian soft tissue coefficient ranges of 0.005–0.05 mm−1 and 0.2–1.5 mm−1, respectively. Fabrication of fluorophore-doped biomimicking and complex geometric structures validated the ability to print feature sizes less than 200 μm. The presented methods and optical characterization techniques provide the foundation for the manufacturing of solid 3D printed fluorescent structures, with direct relevance to biomedical optics and the broad adoption of fast manufacturing methods in fluorescence imaging.

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“…To our knowledge, these parameters have not been measured for bulk gingiva tissue. However, if they were known, there is a procedure to fabricate the appropriate phantom [23]. …”

Section: Methodsmentioning

confidence: 99%

Diffuse optical localization of blood vessels and 3D printing for guiding oral surgery

Bentz

Wu²,

Gaind

et al. 2017

Appl. Opt.

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Diffuse optical imaging through centimeters of tissue has emerged as a powerful tool in biomedical research. However, applications in the operating theater have been limited in part due to data set requirements and computational burden. We present an approach that uses a small number of optical source-detector pairs that allows for the fast localization of arteries in the roof of the mouth and has the potential to reduce complications during oral surgery. The arteries are modeled as multiple-point absorbers, allowing localization of their complex shapes. The method is demonstrated using a printed tissue-simulating mouth phantom. Furthermore, we use the extracted position information to fabricate a custom surgical guide using 3D printing that could protect the arteries during surgery.

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Printed optics: phantoms for quantitative deep tissue fluorescence imaging

Cited by 19 publications

References 18 publications

Cerebral oximetry performance testing with a 3D-printed vascular array phantom

Cerebral oximetry performance testing with a 3D-printed vascular array phantom

3D printing fluorescent material with tunable optical properties

Diffuse optical localization of blood vessels and 3D printing for guiding oral surgery

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