2015
DOI: 10.1016/j.bpj.2015.06.065
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Prion Protein—Antibody Complexes Characterized by Chromatography-Coupled Small-Angle X-Ray Scattering

Abstract: Aberrant self-assembly, induced by structural misfolding of the prion proteins, leads to a number of neurodegenerative disorders. In particular, misfolding of the mostly α-helical cellular prion protein (PrP(C)) into a β-sheet-rich disease-causing isoform (PrP(Sc)) is the key molecular event in the formation of PrP(Sc) aggregates. The molecular mechanisms underlying the PrP(C)-to-PrP(Sc) conversion and subsequent aggregation remain to be elucidated. However, in persistently prion-infected cell-culture models, … Show more

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Cited by 35 publications
(39 citation statements)
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References 78 publications
(118 reference statements)
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“…For comparison, the R g of BvPrP(119-231) and OvPrP(119-231) calculated from the solution NMR structures (3536) were 1.65 and 1.69 nm, respectively; however, these structures lack atomic coordinates for residues 90 to 118. Overall, the molecular dimensions observed for the apo recPrP are in agreement with previous SEC-SAXS studies on MoPrP(89-230) (44). Distance distribution function, P(r) analysis, revealed reduction in the D max values from ~9.4 nm for apo recPrP to ~8.7 nm for Cu(II)-recPrP.…”
Section: Resultssupporting
confidence: 90%
“…For comparison, the R g of BvPrP(119-231) and OvPrP(119-231) calculated from the solution NMR structures (3536) were 1.65 and 1.69 nm, respectively; however, these structures lack atomic coordinates for residues 90 to 118. Overall, the molecular dimensions observed for the apo recPrP are in agreement with previous SEC-SAXS studies on MoPrP(89-230) (44). Distance distribution function, P(r) analysis, revealed reduction in the D max values from ~9.4 nm for apo recPrP to ~8.7 nm for Cu(II)-recPrP.…”
Section: Resultssupporting
confidence: 90%
“…We therefore computed multistate models of the p85␣(1-333) dimer for up to 9 states, using MultiFoXS (42,69). The results show that a weighted combination of five states is sufficient to explain the experimental dimer SAXS profile within its noise ( ϭ 2.623; blue in Fig.…”
Section: Resultsmentioning
confidence: 99%
“…A "multistate model" is a model that specifies multiple discrete structural states of the system, all of which are required to explain the input information (19,(42)(43)(44). In contrast, in an ensemble of models, any single model explains the input information.…”
Section: Integrative Multistate Modeling Of P85␣ and P85␣(1-333) Dimersmentioning
confidence: 99%
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“…Next, we analyze the distribution of R g values for the ensemble of good-scoring multi-state models to estimate the number of possible states [39]. The number of peaks in the R g distribution is a lower-bound estimate on the number of states, and the width of the peak is indicative of the state precision [40]. …”
Section: Methodsmentioning
confidence: 99%