Prion protein gene polymorphisms in classical scrapie-affected flocks of sheep in Central Macedonia

Kioutsioukis, C.; Papadogiannakis, E.; Palaska, Vayia; Kontos, V.; Papakostaki, D.; Paraskeva, S.; Vassalou, Evdokia

doi:10.12681/jhvms.18015

Cited by 1 publication

(2 citation statements)

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“…Our findings showed that the ARQ/ARQ genotype was found predominant (0.40) for Turkish Awassi sheep in this study (Table 3). This observation is coherent with the previous study (0.53) for Awassi sheep in Türkiye [9] as well as reports on other breeds in different countries [21,25,28,32,35,36]. In contrast to Turkish Awassi sheep, the ARQ/ARH genotype was predominant in Palestinian and Saudi Arabian Awassi sheep, with frequencies of 0.36 and 0.48, respectively.…”

Section: Discussionsupporting

confidence: 92%

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Novel Prion Protein Gene Polymorphisms in Awassi Sheep in Three Regions of the Fertile Crescent

Rashaydeh,

Yildiz,

Alharthi

et al. 2023

Veterinary Sciences

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Scrapie is a fatal, neurodegenerative disease that affects sheep and goats, and genetic susceptibility to scrapie in sheep is associated with polymorphisms in the prion protein (PRNP) gene. The aim of this study is to identify PRNP polymorphism in Awassi sheep from Türkiye, the Palestinian Authority, and Saudi Arabia. A total of 150 healthy sheep were genotyped for PRNP, using Sanger sequencing. There were seven alleles and eleven genotypes observed based on codons 136, 154, and 171 of PRNP. The ARQ allele was predominant in all populations. The most resistant allele to scrapie, ARR, was present in all three regions. The VRQ allele, associated with the highest susceptibility to scrapie, was detected only in Türkiye at a low frequency. In this study, twenty-seven amino acid substitutions were found. Eight of them (R40Q, G65E, H88L, S98T, A118P, S138T, V192F and L250I) have not been previously reported. These data indicate that sheep breeds close to the sheep domestication center have maintained high genetic diversity in the PRNP region. Our findings on PRNP will provide valuable insights for sheep breeding programs, aiding in the selection of genotypes resistant to scrapie in Türkiye, the Palestinian Authority, and Saudi Arabia.

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Section: Discussionsupporting

confidence: 92%

“…Thus, scrapie breeding programs should be implemented in these regions to increase the incidence of the ARR allele. Most studies conducted in sheep populations across the world have demonstrated that the predominant allele is ARQ [8,9,27,[29][30][31][32][33][34]. The ARQ allele is thus regarded as the ovine wild-type allele.…”

Section: Discussionmentioning

confidence: 99%