Prioritization of potential vaccine candidates and designing a multiepitope-based subunit vaccine against multidrug-resistant Salmonella Typhi str. CT18: A subtractive proteomics and immunoinformatics approach

Chand, Yamini; Singh, Sachidanand

doi:10.1016/j.micpath.2021.105150

Cited by 21 publications

(10 citation statements)

References 89 publications

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“…Moreover, antiviral peptides (AVPs) have the potential to block a virus’ cycle at different levels from viral attachment to the host cell to its replication. Some AVPs have natural origins [ [4] , [5] , [6] , [7] ] while others are discovered or rationally designed by bioinformatics techniques machine learning [ [8] , [9] , [10] , [11] ]. Since the outbreak of COVID-19, which is caused by the SARS-CoV-2 virus, peptide inhibitors have also been among the promising anti-covid agents from various resources [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of SARS-CoV-2 pathogenesis by potent peptides designed by the mutation of ACE2 binding region

Pourmand

Zareei

Shahlaei

et al. 2022

Computers in Biology and Medicine

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Section: Introductionmentioning

confidence: 99%

Inhibition of SARS-CoV-2 pathogenesis by potent peptides designed by the mutation of ACE2 binding region

Pourmand

Zareei

Shahlaei

et al. 2022

Computers in Biology and Medicine

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“…Multi-epitope-based vaccines were preferred over conventional vaccines due to their cost-effectiveness, superior safety, and the ability to tailor epitopes intelligently for increased potency [89], [90]. Several previous studies have employed similar approaches to design multi-epitope vaccine, however, unlike our present studies, they targeted a particular strain of Salmonella [91]–[94]. Here, to develop a pan- Salmonella vaccine, we targeted the core proteome of Salmonella to design a vaccine that will be effective against a broad spectrum of Salmonella infections.…”

Section: Discussionmentioning

confidence: 99%

An Integrated Comparative Genomics, Subtractive Proteomics and Immunoinformatics Framework for the Rational Design of a Pan-SalmonellaMulti-Epitope Vaccine

Bhattacharjee,

Hosen,

Lamisa

et al. 2023

Preprint

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Salmonella infections are a global public health issue due to the high cost of illness surveillance, prevention, and treatment. In this study, we explored the core proteome in Salmonella to design a multi-epitope vaccine through Subtractive Proteomics and immunoinformatics approaches. A total of 2395 core proteins presents in 30 different strains of Salmonella (reference strain-NZ CP014051) were curated. Utilizing the subtractive proteomics approach on the Salmonella core proteome, Curlin major subunit A (CsgA) was selected as the vaccine candidate. csgA is a conserved gene that is related with biofilm formation. Immunodominant B and T cell epitopes from CsgA were predicted using numerous immunoinformatics tools. T lymphocyte epitopes had adequate population coverage and their corresponding MHC alleles showed significant binding scores after peptide-protein based molecular docking. Afterward, a multiepitope vaccine was constructed with peptide linkers and Human Beta Defensin-2 (as an adjuvant). The vaccine was found to be highly antigenic, non-toxic, non-allergic, and had physicochemical properties. Additionally, Molecular Dynamics Simulation and Immune Simulation demonstrated that the vaccine can bind with Toll Like Receptor 4 and elicit robust immune response. Using in vitro, in vivo, and clinical trials, our results would yield a Pan-Salmonella vaccine that will provide protection against various Salmonella species.

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“…A multi-epitope-based vaccine construct consists of several probable antigenic immunodominant epitopes; hence, such a vaccine can be considered an ideal approach to tackling many bacterial diseases [ 39 ]. During the multi-epitope vaccine construction phase, all the probable antigens that are non-allergic, non-toxin and have good water-soluble capacity were linked with each other through specific GPGPG linkers [ 40 ], and fused with an adjuvant (Cholera toxin B-subunit adjuvant) via an EAAAK linker in order to make the vaccine construct more potent [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

Designing of a Novel Multi-Antigenic Epitope-Based Vaccine against E. hormaechei: An Intergraded Reverse Vaccinology and Immunoinformatics Approach

et al. 2022

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Enterobacter hormaechei is involved in multiple hospital-associated infections and is resistant to beta-lactam and tetracycline antibiotics. Due to emerging antibiotics resistance in E. hormaechei and lack of licensed vaccine availability, efforts are required to overcome the antibiotics crisis. In the current research study, a multi-epitope-based vaccine against E. hormaechei was designed using reverse vaccinology and immunoinformatic approaches. A total number of 50 strains were analyzed from which the core proteome was extracted. One extracellular (curlin minor subunit CsgB) and two periplasmic membrane proteins (flagellar basal-body rod protein (FlgF) and flagellar basal body P-ring protein (FlgI) were prioritized for B and T-cell epitope prediction. Only three filtered TPGKMDYTS, GADMTPGKM and RLSAESQAT epitopes were used when designing the vaccine construct. The epitopes were linked via GPGPG linkers and EAAAK linker-linked cholera toxin B-subunit adjuvant was used to enhance the immune stimulation efficacy of the vaccine. Docking studies of the vaccine construct with immune cell receptors revealed better interactions, vital for generating proper immune reactions. Docked complexes of vaccine with MHC-I, MHC-II and Tool-like receptor 4 (TLR-4) reported the lowest binding energy of −594.1 kcal/mol, −706.7 kcal/mol, −787.2 kcal/mol, respectively, and were further subjected to molecular dynamic simulations. Net binding free energy calculations also confirmed that the designed vaccine has a strong binding affinity for immune receptors and thus could be a good vaccine candidate for future experimental investigations.

show abstract

Prioritization of potential vaccine candidates and designing a multiepitope-based subunit vaccine against multidrug-resistant Salmonella Typhi str. CT18: A subtractive proteomics and immunoinformatics approach

Cited by 21 publications

References 89 publications

Inhibition of SARS-CoV-2 pathogenesis by potent peptides designed by the mutation of ACE2 binding region

Inhibition of SARS-CoV-2 pathogenesis by potent peptides designed by the mutation of ACE2 binding region

An Integrated Comparative Genomics, Subtractive Proteomics and Immunoinformatics Framework for the Rational Design of a Pan-SalmonellaMulti-Epitope Vaccine

Designing of a Novel Multi-Antigenic Epitope-Based Vaccine against E. hormaechei: An Intergraded Reverse Vaccinology and Immunoinformatics Approach

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