Prmt4-mediated methylation of NF-κB is critical for neural differentiation of embryonic stem cells

Niu, Hengli; Xiao, Jiyuan; Ma, Zhong-Xing; Chen, Ling

doi:10.1016/j.bbrc.2020.02.072

Cited by 3 publications

(1 citation statement)

References 29 publications

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“…Moreover, it has been recognized as a key regulator of signal transduction (Blanc & Richard, 2017). Despite being intensively studied as a tumor‐associated factor (H. Cheng et al., 2013; Frietze et al., 2008; Y. R. Kim et al., 2010; Liu et al., 2020; Wang et al., 2014), the number of Carm1 functional analyses in the normal development and differentiation of several cell types has increased (Niu et al., 2020; Selvi et al., 2015; Vu et al., 2013). Our previous study demonstrated that Carm1 controls cell division in PC12 cells (Fujiwara et al., 2006).…”

Section: Introductionmentioning

confidence: 99%

Coactivator‐associated arginine methyltransferase 1 controls oligodendrocyte differentiation in the corpus callosum during early brain development

Ishino

Shimizu

Tohyama

et al. 2022

Developmental Neurobiology

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Protein arginine methylation has been recognized as one of key posttranslational modifications for refined protein functions, mediated by protein arginine methyltransferases (Prmts). Coactivator-associated arginine methyltransferase (Carm1, also known as Prmt4) participates in various cellular events, such as cell survival, proliferation, and differentiation through its protein arginine methylation activities. Carm1 regulates cell proliferation of a neuronal cell line and is reportedly expressed in the mammalian brain. However, its detailed function in the central nervous system, particularly in glial cells, remains largely unexplored. In this study, Carm1 exhibited relatively high expression in oligodendrocyte (OL) lineage cells present in the corpus callosum of the developing brain, followed by a remarkable downregulation after active myelination. The suppression of Carm1 activity by inhibitors in isolated oligodendrocyte precursor cells (OPCs) reduced the number of Ki67-expressing and BrdU-incorporated proliferating cells. Furthermore, Carm1 inactivation attenuated OL differentiation, as determined by the expression of Plp, a reliable myelin-related marker. It also impaired the extension of OL processes, accompanied by a significant reduction in gene expression related to OL differentiation and myelination, such as Sox10, Cnp, Myrf, and Mbp. In addition, OLs co-cultured with embryonic dorsal root ganglia neurons demonstrated that Carm1 activity is required for the appropriate formation of myelin processes and myelin sheaths around neuronal axons, and the induction of the clustering of Caspr, a node of Ranvier structural molecule. Thus, we propose that Carm1 is an essential molecule for the development of OPCs and OLs during brain development.

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Section: Introductionmentioning

confidence: 99%

Coactivator‐associated arginine methyltransferase 1 controls oligodendrocyte differentiation in the corpus callosum during early brain development

Ishino

Shimizu

Tohyama

et al. 2022

Developmental Neurobiology

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Lysine and arginine methylation of transcription factors

Giaimo,

Ferrante,

Borggrefe

2024

Cell. Mol. Life Sci.

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Post-translational modifications (PTMs) are implicated in many biological processes including receptor activation, signal transduction, transcriptional regulation and protein turnover. Lysine’s side chain is particularly notable, as it can undergo methylation, acetylation, SUMOylation and ubiquitination. Methylation affects not only lysine but also arginine residues, both of which are implicated in epigenetic regulation. Beyond histone-tails as substrates, dynamic methylation of transcription factors has been described. The focus of this review is on these non-histone substrates providing a detailed discussion of what is currently known about methylation of hypoxia-inducible factor (HIF), P53, nuclear receptors (NRs) and RELA. The role of methylation in regulating protein stability and function by acting as docking sites for methyl-reader proteins and via their crosstalk with other PTMs is explored.

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The role of protein arginine methyltransferases in kidney diseases

Zhang

Zhuang

2020

Clinical Science

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The methylation of arginine residues by protein arginine methyltransferases (PRMTs) is a crucial post-translational modification for many biological processes, including DNA repair, RNA processing, and transduction of intra- and extracellular signaling. Previous studies have reported that PRMTs are extensively involved in various pathologic states, including cancer, inflammation, and oxidative stress reaction. However, the role of PRMTs has not been well described in kidney diseases. Recent studies have shown that aberrant function of PRMTs and its metabolic products—symmetric dimethylarginine (SDMA) and asymmetric dimethylarginine (ADMA)—are involved in several renal pathological processes, including renal fibrosis, acute kidney injury (AKI), diabetic nephropathy (DN), hypertension, graft rejection and renal tumors. We aim in this review to elucidate the possible roles of PRMTs in normal renal function and various kidney diseases.

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Prmt4-mediated methylation of NF-κB is critical for neural differentiation of embryonic stem cells

Cited by 3 publications

References 29 publications

Coactivator‐associated arginine methyltransferase 1 controls oligodendrocyte differentiation in the corpus callosum during early brain development

Coactivator‐associated arginine methyltransferase 1 controls oligodendrocyte differentiation in the corpus callosum during early brain development

Lysine and arginine methylation of transcription factors

The role of protein arginine methyltransferases in kidney diseases

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