Pro-apoptotic and anticancer properties of Thapring – A Tibetan herbal formulation

Choedon, Tenzin; Dolma, Dawa; Kumar, Vijay

doi:10.1016/j.jep.2011.05.031

Cited by 17 publications

(15 citation statements)

References 23 publications

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“…The activities of a group of metabolism-related pathways were significantly diminished, whereas a group of signaling pathways, including apoptosis and immune-related pathways, were significantly activated in a dose-dependent manner. The regulatory pattern observed in our system is in agreement with the postulated anti-carcinogenic effects of the EEDS, as previously proposed by many reports in which the inhibition of metabolic pathways and the activation of apoptosis or immune-related pathways were the main targets of lung cancer drug development [23, 26, 29, 31, 32]. The observation that the activities of diverse signaling and metabolic pathways appeared in separate clusters implies the existence of a common reciprocal regulatory mechanism.…”

Section: Discussionsupporting

confidence: 90%

“…The MAPK pathway has been widely reported to be involved in the growth and invasion of lung cancer, and this pathway has been used for the development of anti-lung cancer drugs [26–28]. Activation of the apoptosis and p53 pathways is also one of the main targets of anti-lung cancer drugs, including herbal extracts [29–31]. Other functionally related pathways identified in the Up-pattern gene set included immune system- or infectious disease-related pathways, including cytokine-cytokine receptor interaction (KEGG 04060), NOD-like receptor signaling pathway (KEGG 04621), Helicobacter pylori infection (KEGG 05120), and Salmonella infection (KEGG 05132).…”

Section: Discussionmentioning

confidence: 99%

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Gene Expression Profile of the A549 Human Non-Small Cell Lung Carcinoma Cell Line following Treatment with the Seeds ofDescurainia sophia, a Potential Anticancer Drug

Kim

Lee

Park

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Descurainia sophia has been traditionally used in Korean medicine for treatment of diverse diseases and their symptoms, such as cough, asthma, and edema. Our previous results showed that ethanol extract of the seeds of D. sophia (EEDS) has a potent cytotoxic effect on human cancer cells. In this study, we reveal the molecular events that are induced by EEDS treatment in A549 human lung cancer cells. The dose-dependent effect of EEDS on gene expression was measured via a microarray analysis. Gene ontology and pathway analyses were performed to identify functional involvement of genes regulated by EEDS. From gene expression analyses, two major dose-dependent patterns were observed after EEDS treatment. One pattern consisted of 1,680 downregulated genes primarily involved in metabolic processes (FDR < 0.01). The second pattern consisted of 1,673 upregulated genes primarily involved in signaling processes (FDR < 0.01). Pathway activity analyses revealed that the metabolism-related pathways and signaling-related pathways were regulated by the EEDS in dose-dependent and reciprocal manners. In conclusion, the identified biphasic regulatory mechanism involving activation of signaling pathways may provide molecular evidence to explain the inhibitory effect of EEDS on A549 cell growth.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Gene Expression Profile of the A549 Human Non-Small Cell Lung Carcinoma Cell Line following Treatment with the Seeds ofDescurainia sophia, a Potential Anticancer Drug

Kim

Lee

Park

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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“…Most spasms do not last for more than a few moments; however, they can occur frequently during the day and sometimes through the night, which leads to bad sleep quality. Improving sleep quality is another important concern for nursing practice .…”

Section: Discussionmentioning

confidence: 99%

“…22 Chronic pain is also a big problem for paraplegic patients; in the present study, our TCM formula reduced the VAS score significantly for the treated patients compared with the placebo group. Most spasms do not last for more than a few moments 23 ; however, they can occur frequently during the day and sometimes through the night, which leads to bad sleep quality. Improving sleep quality is another important concern for nursing practice.…”

Section: Discussionmentioning

confidence: 99%

Novel medical bathing with traditional Chinese herb formula alleviates paraplegia spasticity

Liu

Meng

et al. 2014

Int J of Nursing Practice

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Paraplegia spasm is a kind of chronic disease which lacks effective treatment; the patients have to endure long-term pain, which is a tough problem for nursing practice. Lots of potential candidate medicines are under investigation, and a new Chinese herb formula is introduced in the current study. In the present study, we chose six different well-known Chinese herbs to form a formula, and boiled them into the water with an optimized ratio to make bath water; 80 paraplegic patients received this medicinal bath, and 80 patients received perfume water bath as placebo group. Compared with placebo control patients, the herb-treated patients have significant reduction in paraplegia spasm, visual analogue scale score, clinician global impression and sleep disorder. This novel six-combined formula traditional medicine could be beneficial for alleviating paraplegia spasm, but the underlying action mechanism deserves further study.

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“…Therefore, alternative anticancer drugs with alleviated toxicity, such as phytochemicals, have attracted much attention recently (Jeong et al, 2011). For example, Choedon et al (2011) have provided evidence to demonstrate Thapring, a Tibetan herbal formulation, is able to kill hepatoma cells by the use of MTT. Thus, the use of natural products in determining effective antitumor agents has been a valuable way to discover new drug candidates (Li et al, 2014;Mishra & Tiwari, 2011).…”

Section: Introductionmentioning

confidence: 99%

A flavonoid compound fromChrysosplenium nudicauleinhibits growth and induces apoptosis of the human stomach cancer cell line SGC-7901

Luo

Yang

et al. 2015

Pharmaceutical Biology

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Context: Gastric cancer remains highly prevalent, but treatment options are limited. Natural products have proved to be a rich source of anticancer drugs. Chrysosplenium nudicaule Ledeb. (Saxifragaceae) is a perennial herb that grows in the highlands of China. It has been used as a traditional Chinese medicine to treat digestive diseases for hundreds of years. Recent studies revealed that this herb had anticancer activity, and the flavonoids were speculated to be the effective components. 6,7,3 0 -Trimethoxy-3,5,4 0 -trihydroxy flavone (TTF) and 5,4 0 -dihydroxy-3,6,3 0 trimethoxy-flavone-7-O-b-D-glucoside (DTFG) are flavonoid compounds isolated from Chrysosplenium nudicaule. Objective: This study examined the effect of TTF and DTFG on SGC-7901 human stomach cancer cell in vitro to determine the anticancer and induction of apoptosis properties of TTF. Materials and methods: The proliferation of cells treated with 32, 16, 8, 4, and 2 lg/mL of TTF or DTFG for 24, 48, and 72 h was assessed by the MTT assay. After being treated with TTF, the apoptosis of SGC-7901 cells was assessed by acridine orange staining, ultrastructure, electrophoresis of DNA fragmentation, and flow cytometry. Results: Results indicated that TTF inhibited the growth of cancer cells with an IC 50 value of 8.33 lg/mL after 72 h incubation. However, DTFG showed no inhibitory effect on the growth of the cancer cell. Further studies on TTF also confirmed that it was able to induce apoptosis of SGC-7901 cells at a concentration as low as 4 lg/mL. Discussion and conclusion: The apoptotic effect of TTF makes it a promising candidate for future chemotherapeutic application in treating stomach cancer.

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Pro-apoptotic and anticancer properties of Thapring – A Tibetan herbal formulation

Cited by 17 publications

References 23 publications

Gene Expression Profile of the A549 Human Non-Small Cell Lung Carcinoma Cell Line following Treatment with the Seeds ofDescurainia sophia, a Potential Anticancer Drug

Gene Expression Profile of the A549 Human Non-Small Cell Lung Carcinoma Cell Line following Treatment with the Seeds ofDescurainia sophia, a Potential Anticancer Drug

Novel medical bathing with traditional Chinese herb formula alleviates paraplegia spasticity

A flavonoid compound fromChrysosplenium nudicauleinhibits growth and induces apoptosis of the human stomach cancer cell line SGC-7901

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