Pro-Fibrogenic and Anti-Inflammatory Potential of a Polyphenol-Enriched Fraction from Annona crassiflora in Skin Repair

Abstract

A polyphenol-enriched fraction from Annona crassiflora fruit peel (PEF-Ac) containing chlorogenic acid, epi-catechin, procyanidins B2 and C1, quercetin-glucoside, kaempferol, and caffeoyl-glucoside was investigated for its anti-inflammatory, pro-angiogenic, and profibrogenic potential in the healing of cutaneous wounds. Four wounds were performed on the back of C57 mice and the lesions were treated with the vehicle (Vaseline and lanolin) and PEF-Ac at concentrations of 2%, 4%, and 6% for 4 and 7 d. Neutrophils and macrophages activities were evaluated indirectly by the activity of myeloperoxidase and N-acetyl-β-D-glycosaminidase, angiogenesis was evaluated by hemoglobin dosing and vessel count in histological sections, and collagen deposition was assessed from histological sections stained with picrosirius red. PEF-Ac demonstrated anti-inflammatory activity, with reduced activities of neutrophil and macrophage in the cutaneous wounds. In addition, there was an increase in the synthesis of types I and III collagen, as well as in the percentage of wound closure, mainly after 4 d of treatment. On the other hand, PEF-Ac did not present an effective pro-angiogenic activity. A. crassiflora fruit peel showed anti-inflammatory and profibrogenic properties, indicating a promising natural source of bioactive molecules for treatment of cutaneous wounds.

A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model

“…A polyphenol-enriched fraction from Annona crassiflora fruit peel containing epi-catechin, procyanidins B2 and C1, quercetin-glucoside, and kaempferol shows anti-inflammatory properties by reducing the activity of neutrophils and macrophages in cutaneous wounds. These compounds also favored the synthesis of collagen and the closure of wounds ( 37 ).…”

Recombinant Protein rP21 from Trypanosoma cruzi has Effect on Inflammation, Angiogenesis and Fibrogenesis in Skin Wound Model C57BL/6 Mouse

“…The measurement of hemoglobin in the wound samples was performed using the Drabkin method [11]. Half a wound of each animal was homogenized in 2.0mL of a hemoglobin-specific chromogenic reagent (Drabkin's reagent-Hemoglobin Labtest kit).…”

“…Neutrophils activity can be detected using the enzyme MPO as a quantitative marker [11,12]. One wound was homogenized in 2.0mL of sodium phosphate buffer, 80 mM at pH = 6 for 15 seconds.…”

“…NAG activity was evaluated using the Bailey technique [11,13] which is based on the hydrolysis of p-nitophenyl N-acetyl-β-Dglucosamine (substrate) by N-acetyl-β-Dglycosaminidase, releasing p-nitrophenol. NAG is a lysosomal enzyme produced by activated macrophages.…”

Wound healing activity of the hydroethanolic extract of the leaves of Maytenus ilicifolia Mart. Ex Reis

“…6 For this reason, a survey of plant extracts remains a viable strategy in the search for new and efficient therapeutic agents for wound repair, as with the use of Zataria multiflora , Teucrium polium , Aloe vera and Annona crassiflora . 7 , 8 , 9 , 10 Maytenus ilicifolia Mart. ex Reissek, popularly known as espinheira santa, is a plant described in traditional medicine with scientific evidence for antiulcerogenic, anti-inflammatory and healing properties.…”