Pro-Inflammatory Cytokines in Sepsis: Biological Studies and Prospects From In Silico Research

Gomes, Andréia Patrícia; Miguel, Paulo Sérgio Balbino

doi:10.4172/2329-6577.1000158

Cited by 6 publications

(11 citation statements)

References 33 publications

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“…The innate immunity is involved in the early detection and elimination of invasive pathogens (De Nardo, ). Innate immunity through the TLRs responds to the pathogens and by activating signaling pathways, produces immune‐mediators such as interferon (IFN), TNF, IL‐6, IL‐8, MIF, and IL‐10 (Takeuchi & Akira, ; Frazao et al ., ; De Nardo, ; Gomes et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Human sperm DNA damage has an effect on immunological interaction between spermatozoa and fallopian tube

et al. 2019

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Background: Toll-like receptors play a crucial role in the immunological interaction between the spermatozoa and fallopian tube and contribute to the ovulation, sperm capacitation, fertilization, and pregnancy. Objectives: To investigate the expression of toll-like receptors and their adaptor molecules and cytokines under the effect of spermatozoa with high DNA fragmentation (high DF) in human fallopian tube cell line (OE-E6/E7) and compare to those in normal spermatozoa. Materials and methods: Fresh semen samples were obtained from 10 unexplained infertile males with high DF (more than 20%) and from 10 healthy donors with a DF less than 3%. After sperm preparation, samples were co-cultured with OE-E6/E7. Toll-like receptors, myeloid differentiation factor 88 (MyD88), TIR domain-containing adapter protein (TIRAP), TIR domain-containing adapter-inducing IFN-b (TRIF), TRIF-related adapter molecule as well as IL-6, IL-8, IFN-b, and TNFa mRNA expression were evaluated by quantitative real-time PCR. Protein levels of these cytokines and chemokines were measured using ELISA method. Results: TLR 1-6 mRNA expression in OE-E6/E7 was significantly higher under the effect of spermatozoa with high DF compared to the spermatozoa with low DF. Furthermore, significantly increased mRNA expression of MyD88, TIRAP, and TRIF was observed in the high DF group compared to the low DF group, except TRIF-related adapter molecule. Moreover, the expression of IL-6 and IL-8 in the high DF group was significantly higher than low DF group, although there was no significant difference in IFN-b and TNFa expression between the groups. Discussion and conclusion: Damage-associated molecular patterns from DNA damage activate TLR signaling pathway in human fallopian tubes and result in the upregulation of inflammatory cytokines and chemokines. This situation may provide pathologic environment for capacitation, fertilization, embryo development, and implantation in female reproductive tract and can be one of the mechanisms of infertility in men with high DF.

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Section: Discussionmentioning

confidence: 97%

Human sperm DNA damage has an effect on immunological interaction between spermatozoa and fallopian tube

et al. 2019

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“…So far two markers such as CRP and Procalcitonin have shown good efficacy in identification and monitoring the progress of sepsis. Both these markers are not ideal but they are helpful in correlation with the patient's clinical situation to establish the diagnosis of sepsis [7][8][9][10]. CRP and procalcitonin are two most used tests in diagnosis and follow up.…”

Section: Sepsis=infection+sirsmentioning

confidence: 99%

Sepsis, an Old Problem for Clinicians

Puca¹

2017

Clin Microbiol

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“…These are directly implicated in the weakening of responses to etiological agents, generating the compensatory anti-inflammatory response syndrome (CARS) (Ward, 2011). This is more intense in situations where the patient survives the disturbances related to systemic inflammation (Gomes et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Protein from Hevea brasiliensis “Hev b 13” latex attenuates systemic inflammatory response and lung lesions in rats with sepsis

et al. 2017

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Sepsis induces a severe systemic inflammatory response that may result in multiple organ dysfunction and death. Studies using a protein derived from natural Hevea brasiliensis (rubber tree) latex, denominated Hev b 13, have demonstrated important anti-inflammatory effects, but no data have been published regarding its effects on sepsis. The aim of this study was to investigate the effects of Hev b 13 on the inflammatory response and lung lesions of septal rats. Male Wistar rats were submitted to cecal ligation and puncture (CLP), randomized into groups and treated with subcutaneously administered doses of 0.5/2.0/3.0 mg/Kg of Hev b 13. Next, animals were subdivided into three different points in time (1, 6 and 24 hours after treatments) for collection of blood samples and euthanasia accompanied by organ removal. Total and differential leukocyte counts, cytokine dosage and histological assessment were analyzed. Treatment with Hev b 13 resulted in a significant decline in total and differential leukocytes as well as suppression of TNF-α and IL-6 production, associated with the increase in IL-10 and IL-4 in plasma and lung tissue. Moreover, it reduced morphological and pathological changes found in the lungs, including neutrophil infiltration, edema and alveolar thickening. The present study concluded that Hev b 13 exerts anti-inflammatory effects and attenuates lung lesions in septal rats, showing potential for clinical application.Keywords: Hev b 13, Hevea brasiliensis, inflammatory response, sepsis. Proteína do latex de Hevea brasiliensis "Hev b 13" atenua resposta inflamatória sistêmica e lesão pulmonar de ratos com sepse ResumoSepse induz uma resposta inflamatória sistêmica grave podendo resultar em disfunção de múltiplos órgãos e morte. Pesquisas utilizando uma proteína derivada do látex natural de Hevea brasiliensis (seringueira), denominada Hev b 13 tem demonstrado importantes efeitos anti-inflamatórios, mas nenhum dado foi publicado dos seus efeitos na sepse. O objetivo deste estudo foi investigar os efeitos da Hev b 13 na resposta inflamatória e na lesão pulmonar de ratos com sepse. Ratos machos da linhagem Wistar foram submetidos a ligação e perfuração do ceco (LPC), randomizados em grupos e tratados com as doses 0,5/2,0/3,0 mg/Kg de Hev b 13 subcutâneo. Após subdividiu-se os animais em três pontos diferentes de tempo (1, 6 e 24 horas após os tratamentos) para coleta de amostras sanguíneas e eutanásia com remoção dos órgãos. Contagem total e diferencial de leucócitos, dosagem de citocinas e avaliação histológica foram analisadas. O tratamento com a Hev b 13 resultou em diminuição significativa de leucócitos totais e diferenciais bem como suprimiu a produção de TNF-α e IL-6, associado ao aumento de IL-10 e IL-4 no plasma e tecido pulmonar.Araújo, L.A. et al.

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Pro-Inflammatory Cytokines in Sepsis: Biological Studies and Prospects From In Silico Research

Cited by 6 publications

References 33 publications

Human sperm DNA damage has an effect on immunological interaction between spermatozoa and fallopian tube

Human sperm DNA damage has an effect on immunological interaction between spermatozoa and fallopian tube

Sepsis, an Old Problem for Clinicians

Protein from Hevea brasiliensis “Hev b 13” latex attenuates systemic inflammatory response and lung lesions in rats with sepsis

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