Pro-inflammatory Cytokines Modulating Osteoclast Differentiation and Function

Seong, Semun; Kim, Jung Ha; Kim, Nacksung

doi:10.4078/jrd.2016.23.3.148

Cited by 12 publications

(9 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The inflammatory response of hPDLSCs to the material was evaluated using ELISA, and the target cytokines were IL-6, IL-8, and TGF-β. IL-6 and IL-8 are pro-inflammatory cytokines that upregulate the inflammatory response and can be associated with inflammatory responses such as postoperative pain after canal obturation [ 15 ]. AH-Plus showed significantly higher pro-inflammatory cytokine levels (IL-6 and IL-8) than those of calcium silicate-based sealers, which is related to AH-Plus showing high toxicity in cell viability tests [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Biocompatibility of Calcium Silicate-Based Sealers and Epoxy Resin-Based Sealer on Human Periodontal Ligament Stem Cells

Kim

Lee

et al. 2020

Materials

View full text Add to dashboard Cite

The aim of this study was to evaluate the biocompatibility of calcium silicate-based sealers (CeraSeal and EndoSeal TCS) and epoxy resin-based sealer (AH-Plus) in terms of cell viability, inflammatory response, expression of mesenchymal phenotype, osteogenic potential, cell attachment, and morphology, of human periodontal ligament stem cells (hPDLSCs). hPDLSCs were acquired from the premolars (n = 4) of four subjects, whose ages extended from 16 to 24 years of age. Flow cytometry analysis showed stemness of hPDLSCs was maintained in all materials. In cell viability test, AH-Plus showed the lowest cell viability, and CeraSeal showed significantly higher cell viability than others. In ELISA test, AH-Plus showed higher expression of IL-6 and IL-8 than calcium silicate-based sealers. In an osteogenic potential test, AH-Plus showed a lower expression level than other material; however, EndoSeal TCS showed a better expression level than others. All experiments were repeated at least three times per cell line. Scanning electronic microscopy studies showed low degree of cell proliferation on AH-Plus, and high degree of cell proliferation on calcium silicate-based sealers. In this study, calcium silicate-based sealers appear to be more biocompatible and less cytotoxic than epoxy-resin based sealers.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of Biocompatibility of Calcium Silicate-Based Sealers and Epoxy Resin-Based Sealer on Human Periodontal Ligament Stem Cells

Kim

Lee

et al. 2020

Materials

View full text Add to dashboard Cite

show abstract

“…Pro-inflammatory cytokines are involved in the up-regulation of inflammatory reactions. There is abundant evidence that certain pro-inflammatory cytokines, such as IL-1β, IL-6, and TNF-α, are involved in the process of pathological pain [23]. It is known that inflammatory processes are initiated and maintained by pro-inflammatory cytokines which have distinct or shared biological activities [24].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Comparison of Biocompatibility of Calcium Silicate-Based Root Canal Sealers

et al. 2019

View full text Add to dashboard Cite

The aim of this study was to assess the effect of three calcium silicate-based sealers (EndoSeal MTA, Nano-ceramic Sealer, and Wellroot ST) and two epoxy resin-based sealers (AH-Plus, AD Seal) on various aspects, such as cell viability, inflammatory response, and osteogenic potential, of human periodontal ligament stem cells (hPDLSCs). AH-Plus showed the lowest cell viability on hPDLSCs in all time periods in fresh media. In set media, hPDLSCs showed no significant differences in cell viability among all the tested materials. Wellroot ST showed the highest level of cell adhesion and the morphology of attached cells. AH-plus presented a significantly higher expression of IL-6 and IL-8 than the other sealers. AD Seal and three calcium silicate sealers showed high expression of the mesenchymal stem cell markers. ALP mRNA expression showed a significant increase in time-dependent manner on all of three calcium silicate-based sealers, which do not seem to interfere with the differentiation of hPDLSCs into osteoblasts. Based on the results from this study, calcium silicate-based sealers appear to be more biocompatible and less cytotoxic than epoxy resin-based sealers. Meanwhile, further and long-term clinical follow-up studies are required.

show abstract

“…Inflammation is closely relevant to bone loss as numerous inflammatory cytokines are recognized to inhibit osteoblastogenesis58 and stimulate osteoclastogenesis 59. Tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1), apart from their direct role in promoting osteoclastogenesis and bone resorption, also stimulate osteoblasts to release other bone-resorbing cytokines such as interleukin-6 (IL-6) and monocyte chemoattractant protein (MCP-1) 60,61.…”

Section: In Vitro Studies and Mechanism Of Action Of Kaempferol As A mentioning

confidence: 99%

<p>The Osteoprotective Effects Of Kaempferol: The Evidence From In Vivo And In Vitro Studies</p>

Wong

Chin

2019

DDDT

126

View full text Add to dashboard Cite

Kaempferol is a dietary bioflavonoid ubiquitously found in various types of plant. It possesses a wide range of medicinal properties suggesting its potential clinical utility that requires further investigation. The present review intends to highlight the efficacy of kaempferol and its molecular mechanisms of action in regulating bone metabolism. Many reports have acknowledged the bone-protecting property of kaempferol and kaempferol-containing plants using in vitro and in vivo experimental models. Kaempferol supplementation showed bone-sparing effects in newborn rats, glucocorticoid-induced and ovariectomy-induced osteoporotic models as well as bone fracture models. It achieves the bone-protective effects by inhibiting adipogenesis, inflammation, oxidative stress, osteoclastic autophagy and osteoblastic apoptosis while activating osteoblastic autophagy. The anti-osteoporotic effects of kaempferol are mediated through regulation of estrogen receptor, bone morphogenetic protein-2 (BMP-2), nuclear factor-kappa B (NF-κB), mitogen-activated protein kinase (MAPK) and mammalian target of rapamycin (mTOR) signaling pathways. In summary, kaempferol exhibits beneficial effects on skeleton, thus is potentially effective for the prophylaxis and treatment of osteoporosis.

show abstract

Pro-inflammatory Cytokines Modulating Osteoclast Differentiation and Function

Cited by 12 publications

References 54 publications

Comparison of Biocompatibility of Calcium Silicate-Based Sealers and Epoxy Resin-Based Sealer on Human Periodontal Ligament Stem Cells

Comparison of Biocompatibility of Calcium Silicate-Based Sealers and Epoxy Resin-Based Sealer on Human Periodontal Ligament Stem Cells

In Vitro Comparison of Biocompatibility of Calcium Silicate-Based Root Canal Sealers

<p>The Osteoprotective Effects Of Kaempferol: The Evidence From In Vivo And In Vitro Studies</p>

Contact Info

Product

Resources

About