Pro-inflammatory effects of aluminum in human glioblastoma cells

Campbell, Arezoo; Yang, Ellen; Tsai-Turton, Mindy; Bondy, Stephen C.

doi:10.1016/s0006-8993(02)02305-3

Cited by 55 publications

(53 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, Al or Al and Cu together caused a decrease in IL-6 secretion in human glial cells after a 3-day exposure while TNF-o was increased at this same time-point. These findings are consistent with those reported previously by our laboratory, where Al caused a decrease in IL-6 secretion after a 6-day treatment [9].…”

Section: Discussionsupporting

confidence: 94%

“…In the present study, human glioblastoma cells exposed for 3 days to Al or Cu showed a significant increase in TNF-o secretion. These findings are consistent with previous studies in which this cytokine increased after a 6-day exposure to Al sulfate in this same cell line [9]. TNF-o mRNA has also been reported to be increased in the brain of mice chronically exposed to Al in drinking water [50] and following Cu treatment in human peripheral blood leukocytes [46].…”

Section: Discussionsupporting

confidence: 93%

“…In the present study, a 3-day exposure to Al increased the rate of proliferation of human glioblastoma cells. This Al-induced increase was also reported after 6 days of treatment in this same cell line and this effect may be due to increased activation of the cells [9]. Co-exposure of cells to Al and Cu produced a synergistic decrease in cell proliferation of human glioblastoma cells.…”

Section: Discussionsupporting

confidence: 76%

“…Our results show that Al and Cu cause a time dependent increase in NF-h:B activation. The effect of Al on NF-1;B may be due to the precipitation of Al in solution and the formation of colloidal species [9]. These large colloids of aluminum may be a source of Al +:i species.…”

Section: Discussionmentioning

confidence: 99%

“…The aluminum (500 /tM) and copper (5 /LM) solutions were prepared in the cell media and immediately sterile-filtered prior to the first dosing. The Al concentration used was based on a previous study showing that at this level the metal causes an inflammatory response in this cell line [9]. The concentration of Cu was chosen based on dose-response assays showing that at 5 /JM, the metal caused a pronounce effect on ROS production without changing cell viability (data not shown).…”

Section: Cell Preparationmentioning

confidence: 99%

See 4 more Smart Citations

Aluminum and copper interact in the promotion of oxidative but not inflammatory events: Implications for Alzheimer's disease

Becaria

Bondy

Campbell

2003

JAD

Self Cite

View full text Add to dashboard Cite

Abstract. The etiology of Alzheimer's Disease (AD) is multifactorial. It has been suggested that transition metals such as copper (Cu) and iron (Fe) as well as aluminum (Al) may be involved in the pathogenesis of the disorder. While Cu and Fe are redox-active, Al only exists in the trivalent form and is redox-inert. We previously demonstrated that Al exposure causes an increase in inflammatory parameters in human glioblastoma T98G cells. In the present study we further demonstrate that co-exposure with Cu exacerbates the oxidative but not inflammatory effects of Al in this cell line. While Cu-induced reactive oxygen species (ROS) production was greatly enhanced in the presence of Al, TNF-a secretion induced by either metal was not further potentiated by simultaneous exposure to Al and Cu. Furthermore, exposure to both metals reduced the individual Al and Cu-induced activation of the immune-related transcription factor NF-,,,;B. Therefore, while synergistic interaction between the two metals increases oxidative events, this does not lead to potentiation of Al-induced inflammation. Thus the ability of aluminum to promote inflammatory processes does not depend on an increase ROS production induced by interaction with transition metals.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Cell Preparationmentioning

confidence: 99%

See 3 more Smart Citations

Aluminum and copper interact in the promotion of oxidative but not inflammatory events: Implications for Alzheimer's disease

Becaria

Bondy

Campbell

2003

JAD

Self Cite

View full text Add to dashboard Cite

show abstract

Chronic exposure to aluminum in drinking water increases inflammatory parameters selectively in the brain

Campbell

Becaria

Lahiri

et al. 2004

J of Neuroscience Research

158

View full text Add to dashboard Cite

A link between aluminum (Al) exposure and age-related neurological disorders has long been proposed. Although the exact mechanism by which the metal may influence disease processes is unknown, there is evidence that exposure to Al causes an increase in both oxidative stress and inflammatory events. These processes have also been suggested to play a role in Alzheimer's disease (AD), and exposure to the metal may contribute to the disorder by potentiating these events. Al lactate (0.01, 0.1, and 1 mM) in drinking water for 10 weeks increased inflammatory processes in the brains of mice. The lowest of these levels is in the range found to increase the prevalence of AD in regions where the concentrations of the metal are elevated in residential drinking water (Flaten [2001] Brain Res. Bull. 55:187-196). Nuclear factor-kappaB as well as tumor necrosis factor-alpha (TNF-alpha) and interleukin 1alpha (IL-1alpha) levels were increased in the brains of treated animals. The mRNA for TNF-alpha was also up-regulated following treatment. Enhancement of glial fibrillary acidic protein levels and reactive microglia was seen in the striatum of Al-treated animals. The level of amyloid beta (Abeta40) was not significantly altered in the brains of exposed animals. Insofar as no parallel changes were observed in the serum or liver of treated animals, the proinflammatory effects of the metal may be selective to the brain. Al exposure may not be sufficient to cause abnormal production of the principal component of senile plaques directly but does exacerbate underlying events associated with brain aging and thus could contribute to progression of neurodegeneration.

show abstract

Micro‐Raman detection of the differentiation state of SH‐SY5Y cells grown on silicon and aluminium substrates

Ricci

Sagini

Caponi

et al. 2018

J Raman Spectroscopy

View full text Add to dashboard Cite

SH‐SY5Y cell line is a model for neural diseases originating from neuroblastoma, a paediatric embryonal malignancy that may differentiate and regress to a benign state. By monitoring the differentiation in single living cells, it could be possible to reach a better understanding of the process and, consequently, to develop new therapeutic approaches. In this work, we investigated the potential of Raman microspectroscopy to monitor biochemical changes in single living SH‐SY5Y cells treated with a combination of retinoic acid (RA) and brain‐derived neurotrophic factor to stimulate the differentiation toward a neuronal phenotype. The results show that the spectroscopic analysis is able to detect biochemical alterations consistent with the differentiation process. Moreover, we investigated also the influence of the cell culture substrate on the treatment outcome. Silicon and aluminium are 2 materials suitable for the spectroscopic analysis and of interest in neuroscience applications. Even though silicon has an ensured biocompatibility, it is subject to alterations after the treatment with RA. Aluminium revealed to be a cost‐effective cell culture substrate for the RA and brain‐derived neurotrophic factor treatment of SH‐SY5Y cells and particularly suitable for Raman microspectroscopy.

show abstract

Pro-inflammatory effects of aluminum in human glioblastoma cells

Cited by 55 publications

References 36 publications

Aluminum and copper interact in the promotion of oxidative but not inflammatory events: Implications for Alzheimer's disease

Aluminum and copper interact in the promotion of oxidative but not inflammatory events: Implications for Alzheimer's disease

Chronic exposure to aluminum in drinking water increases inflammatory parameters selectively in the brain

Micro‐Raman detection of the differentiation state of SH‐SY5Y cells grown on silicon and aluminium substrates

Contact Info

Product

Resources

About