Pro-inflammatory response and genotoxicity caused by clay and graphene nanomaterials in A549 and THP-1 cells

Ianni, Emilio Di; Möller, Peter; Vogel, Ulla; Jacobsen, Nicklas Raun

doi:10.1016/j.mrgentox.2021.503405

Cited by 22 publications

(25 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this work we use toxicological studies in mice, as these represent a suitable method for assessment of the complexity of the pulmonary response to inhaled particles, that is, it allows to detect important toxic properties both at the site of deposition and systemically in distal organs. [33][34][35] The aim of this study was to determine differences in relative toxicity and physicochemical properties of airborne particles inside and outside occupied residences. It was done by on-line characterisation and collection of the airborne particles simultaneously inside and outside 15 occupied residences in Sweden and performing toxicological studies in mice.…”

Section: Practical Implicationsmentioning

confidence: 99%

“…These different types of studies provide complimentary information and are all needed to assess the toxicity and to understand the mechanisms behind the observed health effects. In this work we use toxicological studies in mice, as these represent a suitable method for assessment of the complexity of the pulmonary response to inhaled particles, that is, it allows to detect important toxic properties both at the site of deposition and systemically in distal organs 33–35 …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Indoor PM _2.5 from occupied residences in Sweden caused higher inflammation in mice compared to outdoor PM _2.5

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Practical Implicationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Indoor PM _2.5 from occupied residences in Sweden caused higher inflammation in mice compared to outdoor PM _2.5

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…For this reason, CNMs rank among the most researched substances in toxicology, as well. A regular finding of both in vitro and in vivo toxicological studies is the increased production of inflammatory cytokines, pointing to changes in the activity of the immune system [ 30 , 31 , 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Immunotoxicity of Carbon-Based Nanomaterials, Starring Phagocytes

Švadláková

Holmannová

Koláčková

et al. 2022

IJMS

View full text Add to dashboard Cite

In the field of science, technology and medicine, carbon-based nanomaterials and nanoparticles (CNMs) are becoming attractive nanomaterials that are increasingly used. However, it is important to acknowledge the risk of nanotoxicity that comes with the widespread use of CNMs. CNMs can enter the body via inhalation, ingestion, intravenously or by any other route, spread through the bloodstream and penetrate tissues where (in both compartments) they interact with components of the immune system. Like invading pathogens, CNMs can be recognized by large numbers of receptors that are present on the surface of innate immune cells, notably monocytes and macrophages. Depending on the physicochemical properties of CNMs, i.e., shape, size, or adsorbed contamination, phagocytes try to engulf and process CNMs, which might induce pro/anti-inflammatory response or lead to modulation and disruption of basic immune activity. This review focuses on existing data on the immunotoxic potential of CNMs, particularly in professional phagocytes, as they play a central role in processing and eliminating foreign particles. The results of immunotoxic studies are also described in the context of the entry routes, impacts of contamination and means of possible elimination. Mechanisms of proinflammatory effect depending on endocytosis and intracellular distribution of CNMs are highlighted as well.

show abstract

“… 10 A549 cells have been widely used in reports estimating pulmonary toxicity of NMs, eg silica (SiO 2 ), iron oxides (Fe x O y ), zinc oxide (ZnO), titanium dioxide (TiO 2 ), 11–13 silver (Ag) 14 nanoparticles and other specifically functionalized nanoparticles, 15 nanofibers, 16 , 17 nanosheets 18 and carbon-based nanomaterials. 19 , 20 …”

Section: Introductionmentioning

confidence: 99%

“…10 A549 cells have been widely used in reports estimating pulmonary toxicity of NMs, eg silica (SiO 2 ), iron oxides (Fe x O y ), zinc oxide (ZnO), titanium dioxide (TiO 2 ), [11][12][13] silver (Ag) 14 nanoparticles and other specifically functionalized nanoparticles, 15 nanofibers, 16,17 nanosheets 18 and carbon-based nanomaterials. 19,20 A variety of TiO 2 NMs, eg nanoparticles, 21 nanofibers 17 and nanotubes, 22 has been developed. All these NMs have been evaluated for biological effects because TiO 2 has been used in medicine, material science and industry.…”

Section: Introductionmentioning

confidence: 99%

Evaluating the Use of TiO2 Nanoparticles for Toxicity Testing in Pulmonary A549 Cells

Báčová¹,

Knotek²,

Kopecká³

et al. 2022

IJN

View full text Add to dashboard Cite

Purpose Titanium dioxide nanoparticles, 25 nm in size of crystallites (TiO 2 P25), are among the most produced nanomaterials worldwide. The broad use of TiO 2 P25 in material science has implied a request to evaluate their biological effects, especially in the lungs. Hence, the pulmonary A549 cell line has been used to estimate the effects of TiO 2 P25. However, the reports have provided dissimilar results on caused toxicity. Surprisingly, the physicochemical factors influencing TiO 2 P25 action in biological models have not been evaluated in most reports. Thus, the objective of the present study is to characterize the preparation of TiO 2 P25 for biological testing in A549 cells and to evaluate their biological effects. Methods We determined the size and crystallinity of TiO 2 P25. We used four techniques for TiO 2 P25 dispersion. We estimated the colloid stability of TiO 2 P25 in distilled water, isotonic NaCl solution, and cell culture medium. We applied the optimal dispersion conditions for testing the biological effects of TiO 2 P25 (0–100 µg.mL −1 ) in A549 cells using biochemical assays (dehydrogenase activity, glutathione levels) and microscopy. Results We found that the use of fetal bovine serum in culture medium is essential to maintain sufficient colloid stability of dispersed TiO 2 P25. Under these conditions, TiO 2 P25 were unable to induce a significant impairment of A549 cells according to the results of biochemical and microscopy evaluations. When the defined parameters for the use of TiO 2 P25 in A549 cells were met, similar results on the biological effects of TiO 2 P25 were obtained in two independent cell laboratories. Conclusion We optimized the experimental conditions of TiO 2 P25 preparation for toxicity testing in A549 cells. The results presented here on TiO 2 P25-induced cellular effects are reproducible. Therefore, our results can be helpful for other researchers using TiO 2 P25 as a reference material.

show abstract

Pro-inflammatory response and genotoxicity caused by clay and graphene nanomaterials in A549 and THP-1 cells

Cited by 22 publications

References 51 publications

Indoor PM _2.5 from occupied residences in Sweden caused higher inflammation in mice compared to outdoor PM _2.5

Indoor PM _2.5 from occupied residences in Sweden caused higher inflammation in mice compared to outdoor PM _2.5

Immunotoxicity of Carbon-Based Nanomaterials, Starring Phagocytes

Evaluating the Use of TiO2 Nanoparticles for Toxicity Testing in Pulmonary A549 Cells

Contact Info

Product

Resources

About

Pro-inflammatory response and genotoxicity caused by clay and graphene nanomaterials in A549 and THP-1 cells

Cited by 22 publications

References 51 publications

Indoor PM 2.5 from occupied residences in Sweden caused higher inflammation in mice compared to outdoor PM 2.5

Indoor PM 2.5 from occupied residences in Sweden caused higher inflammation in mice compared to outdoor PM 2.5

Immunotoxicity of Carbon-Based Nanomaterials, Starring Phagocytes

Evaluating the Use of TiO2 Nanoparticles for Toxicity Testing in Pulmonary A549 Cells

Contact Info

Product

Resources

About

Indoor PM _2.5 from occupied residences in Sweden caused higher inflammation in mice compared to outdoor PM _2.5

Indoor PM _2.5 from occupied residences in Sweden caused higher inflammation in mice compared to outdoor PM _2.5