Pro-oxidant- Antioxidant Balance (PAB) in Rheumatoid Arthritis and its Relationship to Disease Activity

Sahebari, Maryam; Shakeri, Farzaneh; Azadi, Hamideh Ghodrati; Arjmand, Mohammad-Hassan; Ghayour‐Mobarhan, Majid; Parizadeh, Mohammad Reza; Alamdari, Daryoush Hamidi

doi:10.2174/1573397111666150522094716

Cited by 9 publications

(6 citation statements)

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“…37,38) Oxidative stress is involved in the pathogenesis of RA. 5) ROS degrade proteoglycans, activate metalloproteinases, and induce apoptosis of chondrocytes, contributing to the damage to cartilage and bone tissue. 39) We demonstrated that OLM reduced MDA levels in the synovium of rats, reflecting an improvement in lipid peroxidation, and increased levels of GSH, an impor- The experimental groups were saline only, zymosan only (Zy), olmesartan (OLM) 15 and 30 mg/kg, and diclofenac sodium (SD) 100 mg/kg.…”

Section: Discussionmentioning

confidence: 99%

“…Patients with RA have high oxidative stress levels and higher malonaldehyde (MDA) levels than do normal patients. 5) Data suggest that an antioxidant diet can reduce RA incidence. 6) RA is considered to be a systemic disease that primarily affects the joints, but inflammatory mediators involved in the pathogenesis of the disease can affect other organs, such as the heart.…”

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confidence: 99%

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Olmesartan Prevented Intra-articular Inflammation Induced by Zymosan in Rats

Guerra

Menezes

Araújo

et al. 2016

Biological & Pharmaceutical Bulletin

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The objective of this study was to study the effect of olmesartan medoxomil (OLM), an antihypertensive drug, on intra-articular inflammation induced by zymosan (Zy) in Wistar rats. Intra-articular inflammation was induced in the right knees of rats by 1 mg Zy dissolved in saline. The animals were divided into the following groups: saline only (oral saline and intra-articular saline); Zy only (intra-articular Zy and oral saline), and intra-articular Zy and oral OLM (5, 15, or 30 mg/kg) or diclofenac sodium (SD; 100 mg/ kg). Twenty-four hours after Zy injection, synovial fluid was collected for total leukocyte counts, blood was collected for biochemical measurements, and synovial tissue was collected for histopathology, immunohistochemistry, immunofluorescence and myeloperoxidase (MPO), malonaldehyde (MDA), and non-protein sulphydryl (NPSH) assays. OLM doses of 15 and 30 mg/kg had protective effects, as evidenced by improved histopathological parameters of synovium, reduced total leukocyte counts, reduced MPO and MDA levels, and increased NPSH group levels compared with the Zy group. OLM reduced immunostaining for cyclooxygenase 2, tumour necrosis factor and interleukin 17 and increased immunostaining for superoxide dismutase and glutathione peroxidase. SD produced similar results. The drugs studied caused no change in biochemical parameters of the animals. OLM showed protective effects in this model of Zy-induced intra-articular inflammation.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Olmesartan Prevented Intra-articular Inflammation Induced by Zymosan in Rats

Guerra

Menezes

Araújo

et al. 2016

Biological & Pharmaceutical Bulletin

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“…A modified PAB was applied based on a previously described method. 13,14 The standard solutions were prepared by mixing varying proportions (0–100%) of 250 µM hydrogen peroxide with 3 mM uric acid (in 10 mM sodium hydroxide). Sixty milligrams of tetramethylbenzidine (TMB) powder was dissolved in 10 mL dimethyl sulphoxide (DMSO); for the preparation of TMB cation, 400 µL of TMB/DMSO was added to 20 mL of acetate buffer (0.05 M buffer, pH 4.5), and then 70 µL of fresh chloramine T (100 mM) solution was added to this 20 mL, mixed well, incubated for 2 hours at room temperature in a dark place; 25 U of peroxidase enzyme solution was added to 20 mL TMB cation, dispensed in 1 mL and kept at 20℃.…”

Section: Methodsmentioning

confidence: 99%

Cathelicidin (LL-37) and its correlation with pro-oxidant, antioxidant balance and disease activity in systemic lupus erythematosus: a cross-sectional human study

Sahebari

Roshandel

Saadati

et al. 2017

Lupus

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Background Cathelicidin (LL-37), an endogenous antimicrobial peptide, has recently been involved in the pathogenesis of autoimmune diseases. To assess whether LL-37 reflects disease activity, we measured serum levels of it in systemic lupus erythematosus (SLE) patients with active and inactive disease compared to healthy controls. LL-37 was also compared between new and old cases. Moreover, the correlation of LL-37 and pro-oxidant, antioxidant balance (PAB) was measured. Methods The study population consisted of 50 SLE patients and 28 healthy controls. Of those, 39 patients had active and 11 patients had inactive disease. Serum levels of LL-37 were measured by ELISA and PAB values by a special method. Results There was no difference in levels of LL-37 between patients and healthy controls (50.9 ± 20.8 vs. 67.7 ± 43.3 ng/ml, P = 0.31). LL-37 did not correlate with SLEDAI and its items in total patients. LL-37 had a positive correlation with SLEDAI in active patients ( P = 0.01, r = 0.4). In active patients (78% of patients), multivariate regression analysis showed significant negative correlation between LL-37 and C3 ( P = 0.01, standardized beta -0.50). No difference was found in levels of PAB between patients and controls (90.4 ± 34.1 vs. 86.9 ± 25.6 HK, P = 0.4).There was no difference in the levels of PAB between patients with active and inactive disease (93.2 ± 34.1 vs. 80.2 ± 33.7 HK, P = 0.27). No correlation was found between levels of PAB and SLEDAI items and total score. However, a positive correlation between the levels of LL-37 and PAB in SLE patients was found ( r = 0.3, P < 0.01). Conclusion Based on this study, serum LL-37 and PAB did not increase in lupus compared with healthy individuals. LL-37 serum values rose in parallel with SLEDAI in active disease. Positive correlation between serum PAB and LL-37 could be a great achievement of this study that may suggest the role of antioxidants in controlling NETosis.

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“…Основний ушкоджуючий механізм вільних радикалів полягає в руйнуванні мембран клітин, модифікації білків та ДНК шляхом перекисного окиснення ліпідів (ПОЛ). Високий рівень ПОЛ сприяє апоптозу кардіоміоцитів і відіграє важливу роль в ремоделюванні міокарда, та розвитку діастолічної дисфункції [3,4,5].…”

Section: зв'язок маркерів оксидативного стресу з діастолічною функцієunclassified

Connection of Oxidative Stress Markers with Diastolic Function of the Left Ventricle in Patients with Arterial Hypertension and Rheumatoid Arthritis

Fushtey¹,

Ryndina²,

Sid³

2019

Ukr. ž. med. bìol. sportu

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Pro-oxidant- Antioxidant Balance (PAB) in Rheumatoid Arthritis and its Relationship to Disease Activity

Abstract: The PAB test can show the oxidative stress in RA patients. Further research should be done to determine the potency of the PAB assay as a tool for monitoring adverse effect of oxidative stress in RA, as well as the effect of antioxidant therapies in the outcomes.

Cited by 9 publications

References 0 publications

Olmesartan Prevented Intra-articular Inflammation Induced by Zymosan in Rats

Olmesartan Prevented Intra-articular Inflammation Induced by Zymosan in Rats

Cathelicidin (LL-37) and its correlation with pro-oxidant, antioxidant balance and disease activity in systemic lupus erythematosus: a cross-sectional human study

Connection of Oxidative Stress Markers with Diastolic Function of the Left Ventricle in Patients with Arterial Hypertension and Rheumatoid Arthritis

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