Pro-oxidative, genotoxic and cytotoxic properties of uranyl ions

Гармаш, С. А.; Smirnova, V. S.; Karp, O. E.; Usacheva, Anna; Berezhnov, Alexey V.; Иванов, В. Э.; Chernikov, A. V.; Брусков, В. И.; Gudkov, Sergey V.

doi:10.1016/j.jenvrad.2012.12.009

Cited by 46 publications

(21 citation statements)

References 38 publications

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“…Our results both support previous reports that uranium exposure leads to DNA damage both in vitro and in human populations, and provides a plausible mechanism for those observations through inhibition of DNA repair proteins. UA alone displayed limited cytotoxicity at levels below 10 μM in keeping with other reports in the literature where cytotoxicity is detected at uranium levels at or greater than 100 μM (Stearns et al, 2005; Periyakaruppan et al, 2007; Thiebault et al, 2007; Milgram et al, 2008; Rouas et al, 2010; Heintze et al, 2011; Orona and Tasat, 2012; Garmash et al, 2014). However, there is evidence that elevated blood uranium levels are associated with increased DNA damage (Popp et al, 2000; Guimaraes et al, 2010; Lourenco et al, 2013) and decreased DNA repair capacity in human populations (Au et al, 1995; Au et al, 1998; Au et al, 2010).…”

Section: Discussionsupporting

confidence: 89%

“…Uranium has been reported to be cytotoxic at high concentrations (>100 μM) in a number of cell lines (Stearns et al, 2005; Coryell and Stearns, 2006; Knobel et al, 2006; Periyakaruppan et al, 2007; Thiebault et al, 2007; Milgram et al, 2008; Periyakaruppan et al, 2009; Darolles et al, 2010; Rouas et al, 2010; Heintze et al, 2011; Orona and Tasat, 2012; Garmash et al, 2014), but there is less published information regarding lower concentrations of uranium. We compared uranyl acetate (UA) toxicity in human embryonic kidney cells (HEK293) and normal human keratinocytes (HEKn).…”

Section: Resultsmentioning

confidence: 99%

“…The radiologic toxicity and carcinogenicity of uranium is well-established, but less is known regarding the toxic mechanisms of uranium that are associated with its heavy metal properties (Craft et al, 2004; Brugge et al, 2005; Miller and McClain, 2007; Briner, 2010; Brugge and Buchner, 2011). Uranium exposure in cell culture causes oxidative stress, DNA damage, and genotoxicity (Periyakaruppan et al, 2007; Periyakaruppan et al, 2009; Garmash et al, 2014). In addition, there is evidence that uranium may disrupt DNA repair.…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of poly(ADP-ribose)polymerase-1 and DNA repair by uranium

Cooper

Dashner

Tsosie

et al. 2016

Toxicology and Applied Pharmacology

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Uranium has radiological and non-radiological effects within biological systems and there is increasing evidence for genotoxic and carcinogenic properties attributable to uranium through its heavy metal properties. In this study, we report that low concentrations of uranium (as uranyl acetate; <10 μM) is not cytotoxic to human embryonic kidney cells or normal human keratinocytes; however, uranium exacerbates DNA damage and cytotoxicity induced by hydrogen peroxide, suggesting that uranium may inhibit DNA repair processes. Concentrations of uranyl acetate in the low micromolar range inhibited the zinc finger DNA repair protein poly(ADP-ribose) polymerase (PARP)-1 and caused zinc loss from PARP-1 protein. Uranyl acetate exposure also led to zinc loss from the zinc finger DNA repair proteins Xeroderma Pigmentosum, Complementation Group A (XPA) and aprataxin (APTX). In keeping with the observed inhibition of zinc finger function of DNA repair proteins, exposure to uranyl acetate enhanced retention of induced DNA damage. Co-incubation of uranyl acetate with zinc largely overcame the impact of uranium on PARP-1 activity and DNA damage. These findings present evidence that low concentrations of uranium can inhibit DNA repair through disruption of zinc finger domains of specific target DNA repair proteins. This may provide a mechanistic basis to account for the published observations that uranium exposure is associated with DNA repair deficiency in exposed human populations.

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Section: Discussionsupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inhibition of poly(ADP-ribose)polymerase-1 and DNA repair by uranium

Cooper

Dashner

Tsosie

et al. 2016

Toxicology and Applied Pharmacology

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“…Acridine orange stains both vital and dead cells, while propidium iodide stains only dead cells. After that, the samples were incubated for 10 min at 37°C [50]. Then the samples were examined under a DM 6000 fluorescence microscope (Leica, Germany).…”

Section: Shape Memory Alloys -Fundamentals and Applicationsmentioning

confidence: 99%

Applications of Nanostructural NiTi Alloys for Medical Devices

Nasakina¹,

Sevostyanov²,

Баикин³

et al. 2017

Shape Memory Alloys - Fundamentals and Applications

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New nanostructural shape memory alloy (55.91 wt% of Ni and 44.03 wt% of Ti) for the production of minimally invasive implantation medical devices (stents) was tested for corrosion resistance under static conditions by dipping it into solutions with various acidities (pH from 1.68 to 9.18) for 2 years, for static mechanical properties and for biocompatibility. The material for investigations was 280-μm wires before and after thermal treatment at 450°C for 15 min in air and surface mechanical treatment. The characteristic image and size of grains were determined using the transmission electron microscope (TEM), and the phase composition; surface morphology; and the layer-bylayer composition were investigated using an X-ray diffractometer; a scanning electron microscope (SEM); and an Auger spectrometer. The nickel release from the investigated nanostructural nitinol is less in comparison with data for microstructural nitinol in a solution of any acidity. Dissolution in the alkali medium is absent. A significant retardation of the nickel ion release (and insignificant concentration as a whole) and the absence of titanium ion release in the weakly acidic and neutral solutions with polished samples are observed. A simultaneous 7-11% increase in strength and plasticity in comparison with microstructural nitinol was attained. Toxicity of samples has not been revealed.

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“…The concentration of hydrogen peroxide was determined by the method of enhanced chemiluminescence in a "luminol/4-iodophenol/peroxidase" system [35,36]. Chemiluminescence intensity was measured by using a Beta-1 liquid scintillation counter (Medapparatura, Moscow Region, Russia) in the photon-counting operation mode [37]; calibration was carried out with hydrogen peroxide samples of known concentration [38].…”

Section: Measurement Of Hydrogen Peroxidementioning

confidence: 99%

Self-oscillating Water Chemiluminescence Modes and Reactive Oxygen Species Generation Induced by Laser Irradiation; Effect of the Exclusion Zone Created by Nafion

Gudkov

Astashev

Брусков

et al. 2014

Entropy

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Samples of water inside and outside an exclusion zone (EZ), created by Nafion swollen in water, were irradiated at the wavelength l = 1264 nm, which stimulates the electronic transition of dissolved oxygen from the triplet state to the excited singlet state. This irradiation induces, after a long latent period, chemiluminescence self-oscillations in the visible and near UV spectral range, which last many hours. It occurs that this effect is EZ-specific: the chemiluminescence intensity is twice lower than that from the bulk water, while the latent period is longer for the EZ. Laser irradiation causes accumulation of H2O2, which is also EZ-specific: its concentration inside the EZ is less than that in the bulk water. These phenomena can be interpreted in terms of a model of decreasing O2 content in the EZ due to increased chemical activity of bisulfite anions (HSO3−), arisen as the result of dissociation of terminal sulfonate groups of the Nafion. The wavelet transform analysis of the chemiluminescence intensity from the EZ and the bulk water gives, that self-oscillations regimes occurring in the liquid after the latent period are the determinate processes. It occurred that the chemiluminescence dynamics in case of EZ is characterized by a single-frequency self-oscillating regime, whereas in case of the bulk water, the self-oscillation spectrum consists of three spectral bands.

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Pro-oxidative, genotoxic and cytotoxic properties of uranyl ions

Cited by 46 publications

References 38 publications

Inhibition of poly(ADP-ribose)polymerase-1 and DNA repair by uranium

Inhibition of poly(ADP-ribose)polymerase-1 and DNA repair by uranium

Applications of Nanostructural NiTi Alloys for Medical Devices

Self-oscillating Water Chemiluminescence Modes and Reactive Oxygen Species Generation Induced by Laser Irradiation; Effect of the Exclusion Zone Created by Nafion

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