Proactive transplantation of human neural stem cells prevents degeneration of striatal neurons in a rat model of Huntington disease

Ryu, Jae K.; Kim, Jean; Cho, Sung J; Hatori, Kozo; Nagai, Atsushi; Choi, Hyun B.; Lee, Min C.; McLarnon, James G.; Kim, Seung Up

doi:10.1016/j.nbd.2004.01.016

Cited by 163 publications

(127 citation statements)

References 43 publications

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“…Rescue of degenerating GABAergic host cells by ciliary neurotrophic factor secreted by neural progenitor cells in the rat quinolinic acid model of Huntington's disease showed a significant decline in apomorphin-induced rotations compared with nontransfected cells (Weinelt et al, 2003). Proactive transplantation of human neural stem cells has also been shown to prevent degeneration of striatal neurons in a rat model of Huntington's disease when injected 1 week before injection of the toxic agent (Ryu et al, 2004). One recent study reports on stable induction of a GABAergic phenotype in a line of ϩ (n ϭ 6) and L1 Ϫ (n ϭ 6) cells.…”

Section: Discussionmentioning

confidence: 99%

Neural Cell Adhesion Molecule L1-Transfected Embryonic Stem Cells Promote Functional Recovery after Excitotoxic Lesion of the Mouse Striatum

Bernreuther¹,

Dihné²,

Johann³

et al. 2006

J. Neurosci.

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We have generated a murine embryonic stem cell line constitutively expressing L1 at all stages of neural differentiation to investigate the effects of L1 overexpression on stem cell proliferation, migration, differentiation, cell death, and ability to influence drug-induced rotation behavior in an animal model of Huntington's disease. L1-transfected cells showed decreased cell proliferation in vitro, enhanced neuronal differentiation in vitro and in vivo, and decreased astrocytic differentiation in vivo without influencing cell death compared with nontransfected cells. L1 overexpression also resulted in an increased yield of GABAergic neurons and enhanced migration of embryonic stem cell-derived neural precursor cells into the lesioned striatum. Mice grafted with L1-transfected cells showed recovery in rotation behavior 1 and 4 weeks, but not 8 weeks, after transplantation compared with mice that had received nontransfected cells, thus demonstrating for the first time that a recognition molecule is capable of improving functional recovery during the initial phase in a syngeneic transplantation paradigm.

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Section: Discussionmentioning

confidence: 99%

Neural Cell Adhesion Molecule L1-Transfected Embryonic Stem Cells Promote Functional Recovery after Excitotoxic Lesion of the Mouse Striatum

Bernreuther¹,

Dihné²,

Johann³

et al. 2006

J. Neurosci.

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“…It is possible that NeuN/BrdU positive neurons might not well developed enough to express more mature phenotypic markers at the time of sacrifice, although neural stem cells showed Calbindin+, NADPH-d+, or GAD+ immunoreactivity even in 2-3 weeks after the transplantation (Ryu et al, 2004;Flax et al, 1998). In addition, prelabeled-BrdU may decay or be lost as NSC proliferates, which limit us to the early time period for the histologic analysis.…”

Section: Discussionmentioning

confidence: 99%

Intravenous administration of human neural stem cells induces functional recovery in Huntington's disease rat model

Lee

Chu

Park

et al. 2005

Neuroscience Research

133

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“…Previously, we have isolated a clonal human neural stem cell line (HB1.F3) that had been immortalized by a retroviral vector encoding the v-myc oncogene [5,8], and this stable human cell line shows multipotent capacity to differentiate into neurons and glial cells [8,18] and ameliorate neurological deficits in animal models of stroke [14 -16], Parkinson's disease [19], Huntington's disease [20,21], and lysosomal storage disease [22] following their transplantation into the brain. In the present study, after transplantation into the brain of ICH mice, transplanted human NSCs were found to migrate extensively from the site of implantation into other anatomical sites and to differentiate into neurons and glial cells and improve functional deficits.…”

Section: Introductionmentioning

confidence: 99%

Brain Transplantation of Immortalized Human Neural Stem Cells Promotes Functional Recovery in Mouse Intracerebral Hemorrhage Stroke Model

et al. 2007

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We have generated stable, immortalized cell lines of human NSCs from primary human fetal telencephalon cultures via a retroviral vector encoding v-myc. HB1.F3, one of the human NSC lines, expresses a normal human karyotype of 46, XX, and nestin, a cell type-specific marker for NSCs. F3 has the ability to proliferate continuously and differentiate into cells of neuronal and glial lineage. The HB1.F3 human NSC line was used for cell therapy in a mouse model of intracerebral hemorrhage (ICH) stroke. Experimental ICH was induced in adult mice by intrastriatal administration of bacterial collagenase; 1 week after surgery, the rats were randomly divided into two groups so as to receive intracerebrally either human NSCs labeled with ␤-galactosidase (n ‫؍‬ 31) or phosphate-buffered saline (PBS) (n ‫؍‬ 30). STEM CELLS 2007;25: 1204 -1212

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Proactive transplantation of human neural stem cells prevents degeneration of striatal neurons in a rat model of Huntington disease

Cited by 163 publications

References 43 publications

Neural Cell Adhesion Molecule L1-Transfected Embryonic Stem Cells Promote Functional Recovery after Excitotoxic Lesion of the Mouse Striatum

Neural Cell Adhesion Molecule L1-Transfected Embryonic Stem Cells Promote Functional Recovery after Excitotoxic Lesion of the Mouse Striatum

Intravenous administration of human neural stem cells induces functional recovery in Huntington's disease rat model

Brain Transplantation of Immortalized Human Neural Stem Cells Promotes Functional Recovery in Mouse Intracerebral Hemorrhage Stroke Model

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