Proapoptotic lipid nanovesicles: Synergism with paclitaxel in human lung adenocarcinoma A549 cells

Joshi, Nitin; Shanmugam, Thanigaivel; Kaviratna, Anubhav; Banerjee, Rinti

doi:10.1016/j.jconrel.2011.07.025

Cited by 23 publications

(27 citation statements)

References 35 publications

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“…Paclitaxel loaded lipid nanovesicles (LN-PTX) were prepared by modified thin film hydration method 21 22 , with DPPC:DOPE in 3:2 molar ratio and paclitaxel:lipid in 1:2 molar ratio. Size distribution of LN-PTX was found to be unimodal with average hydrodynamic diameter of 114 ± 11 nm and polydispersity index of 0.17 ± 0.1 ( Figure 2a ), and was further supported by transmission electron microscopy (TEM) images ( Figure 2b ).…”

Section: Resultsmentioning

confidence: 99%

Endogenous lung surfactant inspired pH responsive nanovesicle aerosols: Pulmonary compatible and site-specific drug delivery in lung metastases

Joshi

Shirsath

Singh

et al. 2014

Sci Rep

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Concerns related to pulmonary toxicity and non-specificity of nanoparticles have limited their clinical applications for aerosol delivery of chemotherapeutics in lung cancer. We hypothesized that pulmonary surfactant mimetic nanoparticles that offer pH responsive release specifically in tumor may be a possible solution to overcome these issues. We therefore developed lung surfactant mimetic and pH responsive lipid nanovesicles for aerosol delivery of paclitaxel in metastatic lung cancer. 100–200 nm sized nanovesicles showed improved fusogenicity and cytosolic drug release, specifically with cancer cells, thereby resulting in improved cytotoxicity of paclitaxel in B16F10 murine melanoma cells and cytocompatibility with normal lung fibroblasts (MRC 5). The nanovesicles showed airway patency similar to that of endogenous pulmonary surfactant and did not elicit inflammatory response in alveolar macrophages. Their aerosol administration while significantly improving the biodistribution of paclitaxel in comparison to Taxol (i.v.), also showed significantly higher metastastes inhibition (~75%) in comparison to that of i.v. Taxol and i.v. Abraxane. No signs of interstitial pulmonary fiborisis, chronic inflammation and any other pulmonary toxicity were observed with nanovesicle formulation. Overall, these nanovesicles may be a potential platform to efficiently deliver hydrophobic drugs as aerosol in metastatic lung cancer and other lung diseases, without causing pulmonary toxicity.

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Section: Resultsmentioning

confidence: 99%

Endogenous lung surfactant inspired pH responsive nanovesicle aerosols: Pulmonary compatible and site-specific drug delivery in lung metastases

Joshi

Shirsath

Singh

et al. 2014

Sci Rep

Self Cite

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“…The plates were dried and 100 μl of 10 mM Tris base was added to each plate and kept for 20 minutes to solubilize the dye. Thereafter, the plates were placed on a shaker to allow mixing and the absorbance (OD) of each sample was measured at 560 nm [16]. Cell viability was measured using the formula: % Viability = Absorbance of sample/Absorbance of control ×100…”

Section: In Vitro Cytotoxicitymentioning

confidence: 99%

Molecular Identification of Probiotic Yeast Strains and Their Characterization

Ragavan

Das

2017

Asian J Pharm Clin Res

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Objective: The objective of this study was to identify the potential yeast isolates at themolecular level and to evaluate their probiotic characteristics.Methods: Molecular characterization was done for five potential probiotic yeast strains. In vitro assays have been conducted to evaluate the probiotic properties such as NaCl tolerance, autoaggregation, and coaggregation. Hemolytic activity, urease activity, and cytotoxicity tests were carried out for safety assay during the characterization of yeast strains. Results:In this study, the yeast strains, viz., LM, MR, GOI, GII2, and WI was identified at the molecular level and named as Yarrowia lipolytica VIT-MN01, Kluyveromyces lactis VIT-MN02, Lipomyces starkeyi VIT-MN03, Saccharomycopsis fibuligera VIT-MN04, Brettanomyces custersianus VIT-MN05, respectively. Maximum autoaggregation (92%) and coaggregation (97 %) were noted in case of L. starkeyi VIT-MN03. All yeast strains showed nonhemolytic activity. In vitro toxicity assay was performed and all the yeast strains showed nontoxic nature. Conclusion:Five yeast strains have been studied for their probiotic characteristics and identified at molecular level. Out of five yeast strains, three strains showed maximum adhesion ability, which is a prerequisite for colonization and protection of gastrointestinal tract. All the yeast strains are validated as a safe bioresources because of their nonhemolytic activities and nonproduction of urease. It can be concluded that the identified yeast strains can serve as promising probiotics in various fields of food industry.

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“…Cardanol derivatives demonstrated high potential applications like surface coatings, [24][25][26] surfactants, [27] and pharmaceuticals. [30,31] Vesicular systems have shown effectiveness as drug nanocarriers in the treatment of breast cancer, [32] human lung cancer, [33,34] and for pancreatic cancer therapy. [30,31] Vesicular systems have shown effectiveness as drug nanocarriers in the treatment of breast cancer, [32] human lung cancer, [33,34] and for pancreatic cancer therapy.…”

Section: Introductionmentioning

confidence: 99%

“…[28,29] Targeted anticancer drug delivery to the infected cells is of fundamental importance in the development of nanomedicine. [30,31] Vesicular systems have shown effectiveness as drug nanocarriers in the treatment of breast cancer, [32] human lung cancer, [33,34] and for pancreatic cancer therapy. [35] Recently, the preparation of multicomponent nanovesicular systems based on renewables like cardanol and cholesterol with embedded minor amounts of various other components has been reported.…”

Section: Introductionmentioning

confidence: 99%

Bio‐based benzoxazines synthesized in a deep eutectic solvent: A greener approach toward vesicular nanosystems

Behalo

Bloise

Mele

et al. 2020

Journal of Heterocyclic Chem

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Proapoptotic lipid nanovesicles: Synergism with paclitaxel in human lung adenocarcinoma A549 cells

Cited by 23 publications

References 35 publications

Endogenous lung surfactant inspired pH responsive nanovesicle aerosols: Pulmonary compatible and site-specific drug delivery in lung metastases

Endogenous lung surfactant inspired pH responsive nanovesicle aerosols: Pulmonary compatible and site-specific drug delivery in lung metastases

Molecular Identification of Probiotic Yeast Strains and Their Characterization

Bio‐based benzoxazines synthesized in a deep eutectic solvent: A greener approach toward vesicular nanosystems

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