Probe-label-free electrochemical aptasensor based on methylene blue-anchored graphene oxide amplification

Chen, Jing Rong; Jiao, Xiao Xia; Luo, Hong Qun; Li, Nian Bing

doi:10.1039/c2tb00267a

Cited by 47 publications

(18 citation statements)

References 25 publications

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“…In such approach, the electrochemical signal is intrinsic from the graphene material, which electroactive oxygen functionalities can be reduced, and the reduction extent correlated to the presence of the specific analyte. Another path has been explored where graphene has been used as “indirect” label instead . The large surface area of the graphene sheets and the fast heterogeneous charge transfer on the material has been exploited for the immobilization and detection of electroactive molecules, whit the graphene label having a role in the enhancement of the electrochemical signal.…”

Section: Discussionmentioning

confidence: 99%

“…Parallel to that strategy, in other works graphene labels have been modified with electroactive species for the generation of the electrochemical signal. For example, Li et al . developed a biosensor for the detection of thrombin in which a DNA aptamer (TBA) was first immobilized on a gold electrode by self‐assembly, followed by graphene oxide (GO) conjugation to the electrode surface.…”

Section: Graphene Used As “Indirect” Label For Signal Enhancementmentioning

confidence: 99%

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Advances on the Use of Graphene as a Label for Electrochemical Biosensors

Bonanni

2020

ChemElectroChem

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There has been an overwhelming interest in the use of graphene and its derivatives for electrochemical biosensors in the last decade. Although the majority of the works describe the use of these materials as platform for the immobilization of the biorecognition element, there is a significant, often unrecognized, research effort that has shown how graphene materials can also beneficially serve as signaling labels for biosensing. Owing to its intrinsic electroactivity and small size, nano‐graphene oxide, for example, has been used for this purpose, where the working signal arises from the reduction of the oxygen functionalities on the material surface. In other approaches, graphene labels modified with electroactive probes were also used to promote the signal generation. This Minireview will illustrate how the unique electrochemical and structural features make graphene a very promising material for the detection of the biorecognition event. In addition, an overview will be provided, showing the plethora of options offered by graphene and its derivatives as labels for signal generation and enhancement.

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Section: Discussionmentioning

confidence: 99%

Section: Graphene Used As “Indirect” Label For Signal Enhancementmentioning

confidence: 99%

Advances on the Use of Graphene as a Label for Electrochemical Biosensors

Bonanni

2020

ChemElectroChem

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“…Chen et al conjugated these labels with capture probes and the labels were decoupled from capture probes once the analyte (thrombin or ATP) was incubated with the biosensor (Fig. 5) (Chen et al , 2013a). In such arrangement, LODs of 110 and 15 pg ml -1 were achieved for thrombin and ATP, respectively.…”

Section: Biosensors Based On Interfacial Graphene Electrochemistrymentioning

confidence: 99%

Graphene as signal amplifier for preparation of ultrasensitive electrochemical biosensors

2015

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Early diagnostics of diseases performed with minimal money and time consumption has become achievable due to recent advances in development of biosensors. These devices use biorecognition elements for selective interaction with an analyte and signal readout is obtained via different types of transducers. Operational characteristics of biosensors have been reported to improve substantially, when a diverse range of nanomaterials was employed. This review presents construction of electrochemical biosensors based on graphene, atomically thin 2D carbon crystals, which is currently intensively studied nanomaterial. The most attractive directions of graphene applications in biosensor preparation are discussed here including novel detection and amplification schemes exploiting graphene's unique electrochemical, physical and chemical properties. The future of graphene-based biosensors is most likely bright, but there is still a lot of work to do to fulfill high expectations.

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“…Furthermore, GO with large negatively charged functional groups can also serve as a carrier for positively charged species through strong electrostatic interactions [1]. Two simple label-free electrochemical aptasensors for thrombin and ATP have been reported, making use of methylene blue-anchored GO as signal amplification nano-platforms and taking advantage of the specific binding affinity of the aptamer towards the target and the different affinities of GO for methylene blue, ssDNA, dsDNA, and G-quadruplex [14]. In this approach, specific aptamers were designed to both specifically recognize the analyte-binding and also bind GO onto the electrode.…”

Section: Carbon Namomaterials Used As Carriers For Signal Elements Inmentioning

confidence: 99%

“…Combining signal amplified DNA sensing and electrochemical transduction provides a new avenue to develop ultrasensitive nucleic acid detection technology widely demanded nowadays for routine analysis, point-of-care (POC) and portable device applications [7,8]. Because of their large surface area, excellent biological compatibility and easy functionalization, carbon-based nanomaterials such as carbon nanotubes (CNTs) and graphene have been widely used as excellent carriers for loading numerous signal elements such as enzymes [9], mimic enzymes [10,11], oligonucleotides [12,13] redox reporters [14,15] or combination [7,8]. These elements can greatly amplify the transduction signals of recognition events and simplify the protocols used in electrochemical DNA assays [1,15].…”

Section: Carbon Namomaterials Used As Carriers For Signal Elements Inmentioning

confidence: 99%

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

Yáñez‐Sedeño

Campuzano

Pingarrón

2017

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Growing demand for developing ultrasensitive electrochemical bioassays has led to the design of numerous signal amplification strategies. In this context, carbon-based nanomaterials have been demonstrated to be excellent tags for greatly amplifying the transduction of recognition events and simplifying the protocols used in electrochemical biosensing. This relevant role is due to the carbon-nanomaterials' large surface area, excellent biological compatibility and ease functionalization and, in some cases, intrinsic electrochemistry. These carbon-based nanomaterials involve well-known carbon nanotubes (CNTs) and graphene as well as the more recent use of other carbon nanoforms. This paper briefly discusses the advantages of using carbon nanostructures and their hybrid nanocomposites for amplification through tagging in electrochemical biosensing platforms and provides an updated overview of some selected examples making use of labels involving carbon nanomaterials, acting both as carriers for signal elements and as electrochemical tracers, applied to the electrochemical biosensing of relevant (bio)markers.

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Probe-label-free electrochemical aptasensor based on methylene blue-anchored graphene oxide amplification

Cited by 47 publications

References 25 publications

Advances on the Use of Graphene as a Label for Electrochemical Biosensors

Advances on the Use of Graphene as a Label for Electrochemical Biosensors

Graphene as signal amplifier for preparation of ultrasensitive electrochemical biosensors

Carbon Nanostructures for Tagging in Electrochemical Biosensing: A Review

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