Probing cellular mechanobiology in three-dimensional culture with collagen–agarose matrices

Ulrich, Theresa A.; Jain, Amit; Tanner, Kandice; MacKay, Joanna L.; Kumar, Sanjay

doi:10.1016/j.biomaterials.2009.10.047

Cited by 289 publications

(307 citation statements)

References 54 publications

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“…A recent study (Yang et al, 2010)on variable pore size collagen gel found that cell migration is hindered by small pore size that invasive distance was not very sensitive in the pore size rang of 5-12µm. At small pore size, a variety of factors, including high ligand density in collagen gel that does not encourage the cell polarity and release seen in mesenchymal migration likely contributes to the limited invasion (Ulrich et al, 2010) whilst very large pore size in scaffolds have insufficient tethers on which to generate traction would also limit cell migration. As a result, many researches in tissue engineering are aimed at obtaining polymeric or bioceramic scaffolds with a very high porosity and simultaneous good control over pore size and morphology (Hou et al, 2000).…”

Section: Pore Size Of Tissue Engineering Scaffoldmentioning

confidence: 99%

Cell Responses to Surface and Architecture of Tissue Engineering Scaffolds

Chang¹,

Wang²

2011

Regenerative Medicine and Tissue Engineering - Cells and Biomaterials

336

305

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Section: Pore Size Of Tissue Engineering Scaffoldmentioning

confidence: 99%

Cell Responses to Surface and Architecture of Tissue Engineering Scaffolds

Chang¹,

Wang²

2011

Regenerative Medicine and Tissue Engineering - Cells and Biomaterials

336

305

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“…Many compositions of the matrix have been studied. One of the widely used constituents is the collagen [225][226][227], eventually mixed with other molecules, such as agarose [228], the ECM proteins tenascin [229] or chondroitin sulfate [230]. Among the ECM components, an important role has been demonstrated for HA, able to regulate the glioma cell phenotype [231] or to increase stem-like properties of glioblastoma cells [232].…”

Section: D Tumoral Cns Cultures On Scaffoldsmentioning

confidence: 99%

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Pizzimenti¹

2014

J. Pediatr. Oncol.

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A large body of evidence indicates that three dimensional (3D) cancer models are superior to two-dimensional (2D) ones in better representing the in vivo phenomena. Indeed, 3D models allow recapitulating in vitro the in vivo features observed in solid tumors (e.g. cell polarity, cell-cell/cell-matrix interactions, biochemical/metabolic gradients, anchorage-independent growth and hypoxia). Moreover, it is well established that the microenvironment plays a fundamental role in regulating tumor development and behavior, including drug resistance. Thus, innovative models able to mimic this complexity represent attractive tools in cancer research. In this review article, we provide a comprehensive review of the application of 3D culture systems in pediatrics' cancer research. In particular, 3D in vitro/ex vivo models of the most common pediatric tumors, such as leukemias, lymphomas and malignancies of the nervous system, will be considered.

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“…Cells frequently respond to mechanical stimuli by adaptively tuning their intrinsic mechanical properties, and significant evidence suggests that this ''mechanoadaptation'' is key to transducing these inputs into biochemical signals that mediate cell behavior. Moreover, because disease states are often accompanied by changes in cell and tissue mechanics, there has been growing interest in using cell mechanical properties as a label-free biomarker (5)(6)(7)(8)(9). As a result, there is much interest in developing platforms to quickly and accurately quantify cellular mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

Measuring Cell Viscoelastic Properties Using a Microfluidic Extensional Flow Device

Guillou

Dahl

Lin

et al. 2016

Biophysical Journal

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The quantification of cellular mechanical properties is of tremendous interest in biology and medicine. Recent microfluidic technologies that infer cellular mechanical properties based on analysis of cellular deformations during microchannel traversal have dramatically improved throughput over traditional single-cell rheological tools, yet the extraction of material parameters from these measurements remains quite complex due to challenges such as confinement by channel walls and the domination of complex inertial forces. Here, we describe a simple microfluidic platform that uses hydrodynamic forces at low Reynolds number and low confinement to elongate single cells near the stagnation point of a planar extensional flow. In tandem, we present, to our knowledge, a novel analytical framework that enables determination of cellular viscoelastic properties (stiffness and fluidity) from these measurements. We validated our system and analysis by measuring the stiffness of cross-linked dextran microparticles, which yielded reasonable agreement with previously reported values and our micropipette aspiration measurements. We then measured viscoelastic properties of 3T3 fibroblasts and glioblastoma tumor initiating cells. Our system captures the expected changes in elastic modulus induced in 3T3 fibroblasts and tumor initiating cells in response to agents that soften (cytochalasin D) or stiffen (paraformaldehyde) the cytoskeleton. The simplicity of the device coupled with our analytical model allows straightforward measurement of the viscoelastic properties of cells and soft, spherical objects.

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Probing cellular mechanobiology in three-dimensional culture with collagen–agarose matrices

Cited by 289 publications

References 54 publications

Cell Responses to Surface and Architecture of Tissue Engineering Scaffolds

Cell Responses to Surface and Architecture of Tissue Engineering Scaffolds

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Measuring Cell Viscoelastic Properties Using a Microfluidic Extensional Flow Device

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