Probing Channelrhodopsin Electrical Activity in Algal Cell Populations

Sineshchekov, Oleg A.; Govorunova, Elena G.; Spudich, John L.

doi:10.1007/978-1-0716-0830-2_6

Cited by 2 publications

(2 citation statements)

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“…1c). In this case the electrodes register a projection of the photocurrent on the direction of the preorienting factor, which allows instantaneous measurements of the orientation degree [22,70,71].…”

Section: Methods For Registration Of Chr Activity In Protist Cellsmentioning

confidence: 99%

“…Finally, the suspension method was used as an indirect approach to study gravitaxis [22] and chemotaxis [75] in C. reinhardtii, and provided a foundation for the development of an express bioassay for detection of water pollution with heavy metals and formaldehyde [76,77]. A more detailed technical description of both methods, the suction pipette technique, and the suspension assay, can be found in the earlier published reviews [25,70,71].…”

Section: Methods For Registration Of Chr Activity In Protist Cellsmentioning

confidence: 99%

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Channelrhodopsins: From Phototaxis to Optogenetics

Govorunova,

Sineshchekov

2023

Biochemistry Moscow

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Channelrhodopsins stand out among other retinal proteins because of their capacity to generate passive ionic currents following photoactivation. Owing to that, channelrhodopsins are widely used in neuroscience and cardiology as instruments for optogenetic manipulation of the activity of excitable cells. Photocurrents generated by channelrhodopsins were first discovered in the cells of green algae in the 1970s. In this review we describe this discovery and discuss the current state of research in the field.

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Section: Methods For Registration Of Chr Activity In Protist Cellsmentioning

confidence: 99%