Probing leukocyte traffic in lymph from oro-nasal mucosae by cervical catheterization in a sheep model

Schwartz-Cornil, Isabelle; Epardaud, Mathieu; Albert, Jean-Pierre; Bourgeois, C.; Gérard, Franck; Raoult, Isabelle; Bonneau, Michel

doi:10.1016/j.jim.2005.06.019

Cited by 16 publications

(12 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1A), possibly due to the passive translocation of virus particles in the inoculum and adsorption/ capture by migrating cells, as previously observed with latex particles (39). Low levels of Seg-10 RNA were detected that were associated with lymph cells during the first 3 days postinoculation (p.i.)…”

Section: Resultssupporting

confidence: 57%

See 1 more Smart Citation

Bluetongue Virus Targets Conventional Dendritic Cells in Skin Lymph

Hemati

Contreras

Urien

et al. 2009

J Virol

Self Cite

View full text Add to dashboard Cite

Bluetongue virus (BTV) is the etiological agent of bluetongue, a hemorrhagic disease of ruminants (particularly sheep), which causes important economic losses around the world. BTV is transmitted primarily via the bites of infected midges, which inject the virus into the ruminant's skin during blood feeding. The virus initially replicates in the draining lymph node and then disseminates to secondary organs where it induces edema, hemorrhages, and necrosis. In this study, we show that ovine conventional dendritic cells (cDCs) are the primary targets of BTV that contribute to the primary dissemination of BTV from the skin to draining lymph nodes. Lymph cDCs support BTV RNA and protein synthesis, as well as the production of infectious virus belonging to several different BTV serotypes, regardless of their level of attenuation. Afferent lymph cell subsets, other than cDCs, showed only marginal levels of BTV protein expression. BTV infection provoked a massive recruitment of cDCs to the sheep skin and afferent lymph, providing cellular targets for infection. Although BTV productively infects cDCs, no negative impact on their physiology was detected. Indeed, BTV infection and protein expression in cDCs enhanced their survival rate. Several serotypes of BTV stimulated the surface expression of the CD80 and CD86 costimulatory molecules on cDCs as well as the mRNA synthesis of cytokines involved in inflammation and immunity, i.e., interleukin-12 (IL-12), IL-1␤, and IL-6. BTV-infected cDCs stimulated antigen-specific CD4 and CD8 proliferation as well as gamma interferon production. BTV initially targets cDCs while preserving their functional properties, reflecting the optimal adaptation of the virus to its host cells for its first spread.

show abstract

Section: Resultssupporting

confidence: 57%

“…CD11c, which is not expressed by pDCs (37), was chosen to sort the lymph cDCs and proceed to BTV RNA detection. As controls, CD4 ϩ T cells, which represent the major subset in afferent lymph (60% of TL cells [39]) were sorted from the same samples (Fig. 1B).…”

Section: Resultsmentioning

confidence: 99%

Bluetongue Virus Targets Conventional Dendritic Cells in Skin Lymph

Hemati

Contreras

Urien

et al. 2009

J Virol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Interestingly, although monocytes/ macrophages and neutrophils have been detected in lymph 32 and draining LNs, [32][33][34] and may be involved in antigen presentation, the mechanisms regulating their movement from peripheral tissues have not been elucidated. Our model would predict that, in the context of microbial infection, this process is mediated, in large part, by TLR control of chemokine receptor switching on these cells.…”

Section: Discussionmentioning

confidence: 99%

A TLR2 ligand suppresses inflammation by modulation of chemokine receptors and redirection of leukocyte migration

McKimmie

Moore

Fraser

et al. 2009

Blood

View full text Add to dashboard Cite

“…We discarded the first 3 h of lymph collection to eliminate the CFSE-positive cells that may have passively transferred into lymph due to the pressure of injection; indeed, we previously showed that the passive translocation of particles ends up within 1 h after injection (48). Whereas no fluorescent cells pos/neg fractions from LDL cells originating from different cannulated sheep were selected by indirect coupling to GAM-IgG-coated magnetic beads using specific primary mAb directed against CD11c (BAQ153A), DEC205 (CC98), B marker (DU2-104), or CD14 (CAM36).…”

Section: Ipc-cd45rb Pos Cells Deposited In Skin Migrated In Afferent mentioning

confidence: 99%

Plasmacytoid Dendritic Cells Migrate in Afferent Skin Lymph

Pascale

Contreras

Bonneau³

et al. 2008

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

Conventional dendritic cells enter lymph nodes by migrating from peripheral tissues via the lymphatic route, whereas plasmacytoid dendritic cells (pDC), also called IFN-producing cells (IPC), are described to gain nodes from blood via the high endothelial venules. We demonstrate here that IPC/pDC migrate in the afferent lymph of two large mammals. In sheep, injection of type A CpG oligodinucleotide (ODN) induced lymph cells to produce type I IFN. Furthermore, low-density lymph cells collected at steady state produced type I IFN after stimulation with type A CpG ODN and enveloped viruses. Sheep lymph IPC were found within a minor BnegCD11cneg subset expressing CD45RB. They presented a plasmacytoid morphology, expressed high levels of TLR-7, TLR-9, and IFN regulatory factor 7 mRNA, induced IFN-γ production in allogeneic CD4pos T cells, and differentiated into dendritic cell-like cells under viral stimulation, thus fulfilling criteria of bona fide pDC. In mini-pig, a CD4posSIRPpos subset in afferent lymph cells, corresponding to pDC homologs, produced type I IFN after type A CpG-ODN triggering. Thus, pDC can link innate and acquired immunity by migrating from tissue to draining node via lymph, similarly to conventional dendritic cells.

show abstract

Probing leukocyte traffic in lymph from oro-nasal mucosae by cervical catheterization in a sheep model

Cited by 16 publications

References 40 publications

Bluetongue Virus Targets Conventional Dendritic Cells in Skin Lymph

Bluetongue Virus Targets Conventional Dendritic Cells in Skin Lymph

A TLR2 ligand suppresses inflammation by modulation of chemokine receptors and redirection of leukocyte migration

Plasmacytoid Dendritic Cells Migrate in Afferent Skin Lymph

Contact Info

Product

Resources

About