Probing local chromatin dynamics by tracking telomeres

Benelli, Rebecca; Weiß, Matthias

doi:10.1016/j.bpj.2022.06.020

Cited by 11 publications

(10 citation statements)

References 30 publications

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“…4). Therefore, the motion of nuclear actin was basically stalled in this case, in line with earlier reports on an irreversible collapse of chromatin to a dense, molten-globule-like configuration [50] and an almost complete immobilization of telomeres [46] at the same condition. In contrast, diluting the cell medium to apply hypoosmotic stress (see Materials and Methods), nuclear actin rods remained mobile but changed their key features significantly (Fig.…”

Section: (A) (B)supporting

confidence: 90%

Intermittent subdiffusion of short nuclear actin rods due to interactions with chromatin

Speckner,

Rehfeldt,

Weiss

2023

Preprint

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The interior of cellular nuclei, the nucleoplasm, is a crowded fluid that is pervaded by protein-decorated DNA polymers, the chromatin. Due to the complex architecture of chromatin and a multitude of associated non-equilbrium processes, e.g. DNA repair, the nucleoplasm can be expected to feature non-trivial material properties and hence anomalous transport phenomena. Here, we have used single-particle tracking on nuclear actin rods, which are important players in DNA repair, to probe such transport phenomena. Our analysis reveals that short actin rods in the nucleus show an intermittent, anti-persistent subdiffusion with clear signatures of fractional Brownian motion. Moreover, the diffusive motion is heterogeneous with clear signatures of an intermittent switching of trajectories between at least two different mobilities, most likely due to transient associations with chromatin. In line with this interpretation, hyperosmotic stress is seen to stall the motion of nuclear actin rods, whereas hypoosmotic conditions yield a reptation-like motion. Our data highlight the local heterogeneity of the nucleoplasm, e.g. distinct biochemical microenvironments and chromosome territories, that need to be taken into account for an understanding of nucleoplasmic transport and the mechanobiology of nuclei.

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Section: (A) (B)supporting

confidence: 90%

Intermittent subdiffusion of short nuclear actin rods due to interactions with chromatin

Speckner,

Rehfeldt,

Weiss

2023

Preprint

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“…The weak correlation between the diffusion exponent and temperature may be found in a recent experiment, e.g., in Ref. [31], where a mild dependence of the diffusion exponent on temperature has been observed in diffusion of telomeres inside cells.…”

Section: Discussionmentioning

confidence: 67%

Conditional entropy and weak fluctuation correlation in nonequilibrium complex systems

Itto

2024

J. Phys.: Conf. Ser.

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“…In addition, a recent experimental work in Ref. [ 51 ] has reported a mild dependence of the diffusion exponent of telomeres in cells on temperature at the statistical level. This fact may imply the existence of the weak correlation between their fluctuations.…”

Section: Discussionmentioning

confidence: 99%

Conditional Entropic Approach to Nonequilibrium Complex Systems with Weak Fluctuation Correlation

Itto

2023

Entropy

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A conditional entropic approach is discussed for nonequilibrium complex systems with a weak correlation between spatiotemporally fluctuating quantities on a large time scale. The weak correlation is found to constitute the fluctuation distribution that maximizes the entropy associated with the conditional fluctuations. The approach is illustrated in diffusion phenomenon of proteins inside bacteria. A further possible illustration is also presented for membraneless organelles in embryos and beads in cell extracts, which share common natures of fluctuations in their diffusion.

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Probing local chromatin dynamics by tracking telomeres

Cited by 11 publications

References 30 publications

Intermittent subdiffusion of short nuclear actin rods due to interactions with chromatin

Intermittent subdiffusion of short nuclear actin rods due to interactions with chromatin

Conditional entropy and weak fluctuation correlation in nonequilibrium complex systems

Conditional Entropic Approach to Nonequilibrium Complex Systems with Weak Fluctuation Correlation

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