Probing of chain conformations in conjugated polymer nanoparticles by electron spin resonance spectroscopy

Hintze, Christian; Schütze, Friederike; Drescher, Malte; Mecking, Stefan

doi:10.1039/c5cp05749c

Cited by 14 publications

(21 citation statements)

References 56 publications

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“…This allows for the reliable and efficient fabrication of the 3-nm diameter Pdots with very high colloidal stability. Reduced surface energy of the CP chains in a bent conformation inside the Pdots might also contribute to the high stability of the particles34.…”

Section: Discussionmentioning

confidence: 99%

Controlling photophysical properties of ultrasmall conjugated polymer nanoparticles through polymer chain packing

2017

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Applications of conjugated polymer nanoparticles (Pdots) for imaging and sensing depend on their size, fluorescence brightness and intraparticle energy transfer. The molecular design of conjugated polymers (CPs) has been the main focus of the development of Pdots. Here we demonstrate that proper control of the physical interactions between the chains is as critical as the molecular design. The unique design of twisted CPs and fine-tuning of the reprecipitation conditions allow us to fabricate ultrasmall (3.0–4.5 nm) Pdots with excellent photostability. Extensive photophysical and structural characterization reveals the essential role played by the packing of the polymer chains in the particles in the intraparticle spatial alignment of the emitting sites, which regulate the fluorescence brightness and the intraparticle energy migration efficiency. Our findings enhance understanding of the relationship between chain interactions and the photophysical properties of CP nanomaterials, providing a framework for designing and fabricating functional Pdots for imaging applications.

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Section: Discussionmentioning

confidence: 99%

Controlling photophysical properties of ultrasmall conjugated polymer nanoparticles through polymer chain packing

2017

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“…A dilute copolymer solution in a water-miscible solvent was added to rigorously stirred water, resulting in the formation of core-shell structures. 70,169 As an alternative pathway towards well-defined superstructures, the slow mixing of various solvents with different quality regarding the solubilization of the individual copolymer segments effected homogeneous particle nucleation, followed by the self-organization of stable and fluorescent micelles. 141,160,162 Induced by to the mutual repulsion of the different blocks and the packing constraints due to the connection of the blocks, microphase separation into hydrophilic and hydrophobic domains occurred.…”

Section: Amphiphilic Block Copolymersmentioning

confidence: 99%

Origin of the Anisotropy and Structure of Ellipsoidal Poly(fluorene) Nanoparticles

Sickinger

Mecking

2021

Macromolecules

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An erster Stelle gilt mein Dank Prof. Dr. Stefan Mecking für die Aufnahme in die Arbeitsgruppe und die hervorragende Betreuung dieser Arbeit. Er vertraute mir ein extrem vielseitiges, interessantes und herausforderndes Thema an und zeigte stets Interesse an meiner Forschung. Für seine jahrelange Unterstützung, viele hilfreichen Gespräche und die mir gewährten Freiheiten möchte ich mich herzlich bedanken. Weiterhin danke ich Prof. Dr. Malte Drescher für die Übernahme der Zweitbetreuung und des Zweitgutachtens genauso wie für die gute Zusammenarbeit im Rahmen des Kooperationsprojektes. Für die Übernahme des Prüfungsvorsitzes möchte ich Prof. Dr. Helmut Cölfen ganz herzlich danken. Ein besonders großer Dank gilt Dr. Julian Ruiz Perez für die umfangreiche und geduldige Einführung in die Thematik der Polyfluoren-Nanopartikel, einschließlich zahlreicher Synthese-und Analytikmethoden.

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“…Pioneered by Hubbell and co-workers more than 25 years ago, 1,2 this technique is widely used for studying the structure and function of macromolecules by EPR spectroscopy. 3 This includes proteins, but also synthetic polymers, 4,5 nucleic acids 6 and lipids. 7 Though natural paramagnetic centers such as metal ions are present as cofactors in certain classes of proteins and can be used as probes for EPR spectroscopy, most proteins are diamagnetic and thus EPR silent.…”

Section: Introductionmentioning

confidence: 99%

Site-directed spin labeling of proteins for distance measurements in vitro and in cells

et al. 2016

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Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy allows studying the structure, dynamics, and interactions of proteins via distance measurements in the nanometer range. We here give an overview of available spin labels, the strategies for their introduction into proteins, and the associated potentials for protein structural studies in vitro and in the context of living cells.

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Probing of chain conformations in conjugated polymer nanoparticles by electron spin resonance spectroscopy

Cited by 14 publications

References 56 publications

Controlling photophysical properties of ultrasmall conjugated polymer nanoparticles through polymer chain packing

Controlling photophysical properties of ultrasmall conjugated polymer nanoparticles through polymer chain packing

Origin of the Anisotropy and Structure of Ellipsoidal Poly(fluorene) Nanoparticles

Site-directed spin labeling of proteins for distance measurements in vitro and in cells

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