Probing Phoretic Transport of Oxidative Enzyme-Bound Zn(II)-Metallomicelle in Adenosine Triphosphate Gradient via a Spatially Relocated Biocatalytic Zone

Priyanka,; Maiti, Subhabrata

doi:10.1021/acs.langmuir.4c01401

Langmuir

2024

DOI: 10.1021/acs.langmuir.4c01401

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Probing Phoretic Transport of Oxidative Enzyme-Bound Zn(II)-Metallomicelle in Adenosine Triphosphate Gradient via a Spatially Relocated Biocatalytic Zone

Priyanka,

Subhabrata Maiti

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2024

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Cited by 2 publications

References 64 publications

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Spatial Shifting of Biocondensate Assembly Zone in a Microfluidic Gradient of Dissipative Condition

Juneja,

Sivoria,

Maiti

2024

ChemSystemsChem

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Pursuing non‐equilibrium chemistry with (bio)molecules is of utmost importance for the design of life‐like dynamic materials that emerge in a constant flux of energy. Herein, we explore spatial localization of a dissipative self‐assembly of biocondensate (DNA‐histone) via passing chemical fuel (histone) and one fuel‐degrading agent (trypsin) through two arms of the Y‐shaped microfluidic chip. In this case, continuous supply of fuel and fuel‐degrading agent results self‐assembly of biocondensate, maintaining a non‐equilibrium steady state (NESS). We find in the presence of gradient of dissipating conditions, the formation zone of biocondensate drifts towards fuel‐rich zone (away from dissipating zone). In absence of fuel‐degrading agent, diffusive transport of free DNA towards histone channel (perpendicular to advection) is restricted as it formed much larger micron‐sized biocondensate at the center of the channel (the meeting point of two flows). However, this sidewise DNA diffusion is operative in the presence of fuel‐degrading agent and therefore, the formation zone shifted to histone‐rich zone. Furthermore, we demonstrate that in the presence of trypsin, catalytic DNA’s peroxidase reactivity can be moved to histone‐rich region. Transposition of self‐assembly process in a gradient of dissipative conditions will be of importance in the development of spatially‐controlled chemistry, reaction‐diffusion processes.

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Spatial Shifting of Biocondensate Assembly Zone in a Microfluidic Gradient of Dissipative Condition

Juneja,

Sivoria,

Maiti

2024

ChemSystemsChem

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Salt Gradient-Induced Phoresis of Vesicles and Enhanced Membrane Fusion in a Crowded Milieu

Yadav,

Sivoria,

Maiti

2024

J. Phys. Chem. B

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Phoresis of biocolloidal objects in response to chemical gradients is a matter of interest among diverse scientific disciplines owing to their importance in the spatiotemporal orchestration of biochemical processes. Although there are reports of soft matter transport/phoresis in the gradient of ions or salts in the aqueous system, their phoretic behavior in the presence of macromolecular crowder is largely unexplored. Notably, cellular cytoplasm is illustrated as a crowded milieu and thereby understanding biomolecular phoresis in the presence of polymeric macromolecules would endorse phoretic behavior in a biomimetic environment. Here, we report the phoresis-induced enhanced aggregation and fusion of vesicles in gradients of monovalent (NaCl) and divalent salt (MgCl 2 ), in the presence of polymeric crowder, polyethylene glycol of molecular weight 400 (PEG 400). Apart from diffusiophoresis, depletion force plays a crucial factor in crowded environments to control localized vesicle aggregation in a salt gradient. This demonstration will potentially show the pathway to future research related to spatiotemporally correlated liposomal transport and membrane-dependent function (such as content mixing and signaling) in a physiologically relevant crowded environment.

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Probing Phoretic Transport of Oxidative Enzyme-Bound Zn(II)-Metallomicelle in Adenosine Triphosphate Gradient via a Spatially Relocated Biocatalytic Zone

Cited by 2 publications

References 64 publications

Spatial Shifting of Biocondensate Assembly Zone in a Microfluidic Gradient of Dissipative Condition

Spatial Shifting of Biocondensate Assembly Zone in a Microfluidic Gradient of Dissipative Condition

Salt Gradient-Induced Phoresis of Vesicles and Enhanced Membrane Fusion in a Crowded Milieu

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