Probing structure–antifouling activity relationships of polyacrylamides and polyacrylates

Zhao, Chao; Zhao, Jun; Li, Xiaosi; Wu, Jiang; Chen, Shenfu; Chen, Qiang; Wang, Qiuming; Gong, Xiong; Li, Lingyan; Zheng, Jie

doi:10.1016/j.biomaterials.2013.03.028

Cited by 79 publications

(112 citation statements)

References 52 publications

Supporting

Mentioning

106

Contrasting

Order By: Relevance

“…MD simulations of a 16-mer of polyAAEE in a cubic TIP3P water box of 60 × 60 × 60 Å 3 were performed using the NAMD program 45 with CHARMM22 force fields 46 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 13 AAEE monomers were then initiated, polymerized, and grafted onto ω-mercaptoundecyl bromoisobutyrate SAM-coated gold substrates via the SI-ATRP method (Scheme 2b). The SI-ATRP method has been widely used to graft a variety of antifouling polymers on different substrates to form effective antifouling surfaces with well-controlled film thickness and high packing density, including polyzwitterinoic-based polySBMA 48 and polyCBMA 13 , polyacrylatebased polyHEMA 33 , polyHPMA 40 and polyHEA 35 , and polyacrylamide-based polyHEAA 34 .…”

Section: Molecular Dynamics Simulations Of Polyaaeementioning

confidence: 99%

“…These polymers only differ by a pendant group on the same monomeric backbone (Scheme 1). We have systematically studied these polymers for their antifouling performance using the same experimental protocols [33][34][35]40 . To make fair comparison, here we confined the antifouling comparison to nonspecific protein adsorption on different polymer brushes under their optimal film thicknesses.…”

Section: Structure-antifouling Activity Relationship Of Hydroxy-acrylmentioning

confidence: 99%

“…The short-term stability polyacrylate-based polyHEMA and polyHPMA relative to polyacrylamidebased polyHEAA is likely attribute to ease to hydrolysis degradation of the ester bonds in polyacrylate compared to the amide bonds in polyacrylamide. Further, we also found that two polyacrylamide polymers with amide groups (polySBAA and polyHEAA) exhibited the improved surface hydration and antifouling ability as compared to two corresponding polyacrylate polymers with ester groups (polySBMA and polyHEA) 35 .…”

Section: Introductionmentioning

confidence: 95%

“…35 Such minor difference in protein adsorption could likely be attributed to their subtle structural difference. It was further shown that polyHEAA hydrogels always had higher equilibrium water content than polyHEA hydrogels.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Synthesis and Characterization of Antifouling Poly(N-acryloylaminoethoxyethanol) with Ultralow Protein Adsorption and Cell Attachment

et al. 2014

Self Cite

View full text Add to dashboard Cite

Rational design of effective antifouling polymers is challenging but important for many fundamental and applied applications. Herein we synthesize and characterize an N-acryloylaminoethoxyethanol (AAEE) monomer, which integrates three hydrophilic groups of hydroxyl, amide, and ethylene glycol in the same material. AAEE monomers were further grafted and polymerized on gold substrates to form polyAAEE brushes with well-controlled thickness via surface-initiated atomic transfer radical polymerization (SI-ATRP), with particular attention to a better understanding of the molecular structure-antifouling property relationship of hydroxyl-acrylic-based polymers. The surface hydrophilicity and antifouling properties of polyAAEE brushes as a function of film thickness are studied by combined experimental and computational methods including surface plasmon resonance (SPR) sensors, atomic force microscopy (AFM), cell adhesion assay, and molecular dynamics (MD) simulations. With the optimal polymer film thicknesses (∼10-40 nm), polyAAEE-grafted surfaces can effectively resist protein adsorption from single-protein solutions and undiluted human blood plasma and serum to a nonfouling level (i.e., <0.3 ng/cm(2)). The polyAAEE brushes also highly resist mammalian cell attachment up to 3 days. MD simulations confirm that the integration of three hydrophilic groups induce a stronger and closer hydration layer around polyAAEE, revealing a positive relationship between surface hydration and antifouling properties. The molecular structure-antifouling properties relationship of a series of hydroxyl-acrylic-based polymers is also discussed. This work hopefully provides a promising structural motif for the design of new effective antifouling materials beyond traditional ethylene glycol-based antifouling materials.

show abstract

Section: Molecular Dynamics Simulations Of Polyaaeementioning

confidence: 99%

Section: Structure-antifouling Activity Relationship Of Hydroxy-acrylmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

mentioning

confidence: 99%

See 2 more Smart Citations

Synthesis and Characterization of Antifouling Poly(N-acryloylaminoethoxyethanol) with Ultralow Protein Adsorption and Cell Attachment

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, PRD1 contains lipids in its protein coat and is thus expected to be relatively hydrophobic whereas T4 is a hydrophilic phage. 33 The relative hydrophobicity of PRD1 could decrease its interaction with the interferents in the medium 34 , hence explaining the lower effect of interferents on the efficiency of immobilized PRD1. Moreover, T4 is an asymmetric phage that must be immobilized on the surface with its tail fibers oriented away from the surface to capture bacteria.…”

Section: Bacteria Capture On Phage-functionalized Surfacesmentioning

confidence: 99%

Effects of Environmental and Clinical Interferents on the Host Capture Efficiency of Immobilized Bacteriophages

2014

View full text Add to dashboard Cite

The concept of using bacteriophages to control populations of pathogenic bacteria is gaining momentum, driven mainly by the growing global crisis over antibiotic resistance in both the natural environment and healthcare settings. Bacteriophages (phages) are natural predators of bacteria and are innocuous to humans, animals or plants.Functionalizing surfaces with phage offers the promise of designing devices that can actively capture and deactivate bacteria such as water filters, wound dressings or antibacterial coatings. Our laboratory has previously proven the feasibility of this idea in a clean water matrix, demonstrating that phage-functionalized surfaces are promising candidates for selective capture and inactivation of bacterial pathogens. However, the complex composition of many natural samples (e.g., surface waters, waste water, blood, etc.) can potentially interfere with the interaction of phage and its bacterial host, leading to a decline in the efficiency of the phage-functionalized surface. In this study, the bacterial capture efficiency of phage-functionalized surfaces was assessed in the presence of potential environmental and biomedical interferents. Two phage-bacteria systems were used in this study, namely PRD1 with Salmonella Typhimurium and T4 with Escherichia coli. The potential environmental interferents tested were humic and fulvic acids, colloidal latex microspheres (as a model for environmental colloids), extracellular polymeric substances (EPS), as well as a natural unfiltered groundwater. Albumin, fibrinogen, and blood serum were also tested as representative interferents of interest for biomedical applications such as wound dressings. The inactivation of the selected phages by the potential interferents was first evaluated for each phage suspended in aqueous media containing each interferent. Next, the bacterial (host) capture efficiency of a iii phage-functionalized substrate was evaluated in the presence of each interferent.Interestingly, humic and fulvic acids reduced the capture efficiency of T4-functionalized surfaces by over 60%, even though they did not lead to inactivation of the suspended virions. Neither humics nor fulvics affected the capture efficiency of PRD1. EPS and human serum decreased the host capture efficiency for immobilized PRD1 and T4 by over 70%, and also impaired the infectivity of the non-immobilized (planktonic) phage, although to a much lower extent (less than 50%). The fundamental mechanisms leading to the observed decrease in performance of the phage-functionalized surfaces in the presence of selected interferents is discussed in detail in the thesis. These findings demonstrate the inadequacy of traditional phage selection methods (i.e., infectivity of suspended phage towards its host in clean buffer) for designing antimicrobial surfaces

show abstract

Comparison of carboxybetaine with sulfobetaine polyaspartamides: Nonfouling properties, hydrophilicity, cytotoxicity and model nanogelation in an inverse miniemulsion

Hladysh

Oleshchuk

Dvořáková

et al. 2021

J of Applied Polymer Sci

View full text Add to dashboard Cite

Zwitterionic polymers are promising non‐fouling materials widely used for surface modification. Here, we report the synthesis of zwitterionic polyaspartamides bearing carboxybetaine and sulfobetaine prepared via combining the ring‐opening reaction of polysuccinimide with 3‐(dimethylamino)‐1‐propylamine and the reaction of the formed tertiary amine side groups with 1,3‐propanesultone and 4‐bromobutyric acid, respectively. Both sulfobetaine and carboxybetaine polyaspartamides have cationic and anionic charged groups on the same repeat unit, but they differ in negatively charged groups. The chemical structures of the synthesized polymers were confirmed by 1H NMR and FT‐IR spectroscopies. The zwitterionic property of polyaspartamides were characterized by zeta potential measurements using wide range of pH. The surface properties mainly anti‐protein adsorption abilities and hydrophilicity were tested by surface plasmon resonance analysis, fluorescence microscopy, and contact angle measurements. Importantly, sulfobetaine polyaspartamides demonstrated better both antifouling performance and ability to form more hydrophilic surfaces than carboxybetaine polyaspartamide. Two zwitterionic carboxybetaine and sulfobetaine nanogels were prepared by horseradish peroxidase‐mediated crosslinking in an inverse miniemulsion. Results from dynamic light scattering and transmission electron microscopy showed the formation of nanosized spherical particles. Additionally, sulfobetaine and carboxybetaine polyaspartamides had remarkable biocompatibilities, indicating their promise as non‐fouling materials for biomedical applications.

show abstract

Probing structure–antifouling activity relationships of polyacrylamides and polyacrylates

Cited by 79 publications

References 52 publications

Synthesis and Characterization of Antifouling Poly(N-acryloylaminoethoxyethanol) with Ultralow Protein Adsorption and Cell Attachment

Synthesis and Characterization of Antifouling Poly(N-acryloylaminoethoxyethanol) with Ultralow Protein Adsorption and Cell Attachment

Effects of Environmental and Clinical Interferents on the Host Capture Efficiency of Immobilized Bacteriophages

Comparison of carboxybetaine with sulfobetaine polyaspartamides: Nonfouling properties, hydrophilicity, cytotoxicity and model nanogelation in an inverse miniemulsion

Contact Info

Product

Resources

About