Probing the binding mechanism of photoresponsive azobenzene polyamine derivatives with human serum albumin

Abstract

Representative scheme showing the binding pathway of light-responsive azobenzenes towards human serum albumin.

Two-Photon Macromolecular Probe Based on a Quadrupolar Anthracenyl Scaffold for Sensitive Recognition of Serum Proteins under Simulated Physiological Conditions

“…Due to Ant-PIm absorption at the excitation and emission wavelengths, an inner filter correction was applied before quantitatively analyzing the data using the following equation. 49 where F corr and F obs are the corrected and observed fluorescence, respectively, and A ex and A em are the absorbance values at the excitation and emission wavelengths, respectively.…”

“…K S is the Stern−Volmer constant used in the presence of a static quenching mechanism, [Q] is the Ant-PIm concentration, k q is the apparent quenching rate constant of the biomolecules, and τ 0 is the average excited-state lifetime of HSA without a quencher and it is equal to 3.53 × 10 −9 s. 47,48 Due to Ant-PIm absorption at the excitation and emission wavelengths, an inner filter correction was applied before quantitatively analyzing the data using the following equation. 49…”

Non-toxic Polymeric Dots with the Strong Protein-Driven Enhancement of One- and Two-Photon Excited Emission for Sensitive and Non-destructive Albumin Sensing

“…A particular importance is given to optical detection through such effects as absorption and one- and two-photon excitation (OPE and TPE, respectively) emission processes. In the case of HSA/BSA albumins, considerable attention has been paid to the study of interactions between proteins and optically active agents (probes) such as organic molecules, − supramolecular assemblies, inorganic nanostructures, ,− metallic nanoparticles, , and carbon dots. , …”

Site-Selective Oxidative Coupling Reaction of Diamines toward Aminoazo Compounds

“…As a kind of common industrial material, azo compounds are extensively used as dyes, pigments, drugs, and energetic materials. − While most monoamines can be easily coupled to azo products without worrying much about byproducts, the oxidative coupling of polyamines not only forms multiple intermediates and excessive oxidation byproducts but also may lead to a variety of isomers (Figure A), − thus creating formidable barriers for a simple and low-cost synthesis of aminoazo compounds. Therefore, developing a direct synthetic route for a selective oxidative coupling of polyamines can efficiently increase the construction speed, allowing for easier industrial production, and is thus considered both challenging and rewarding …”