Probing the conjugation of epigallocatechin gallate with β-lactoglobulin and its in vivo desensitization efficiency

Abstract

Epigallocatechin gallate (EGCG) and β-lactoglobulin (βLg) were conjugated by covalent bonds to form a EGCG-βLg conjugates. This conjugation causes structural and bioactivity changes in βLg, which in turn can be...

Effect of ultrasound on cyanidin‐3‐O‐glucoside and β‐lactoglobulin binding interaction and functional properties

“…Intrinsic fluorophores, namely tryptophan, tyrosine and phenylalanine, exhibit strong fluorescence characteristics at a certain excitation wavelength (Jiang et al ., 2018). When the ligand is accessible to proteins, resonance energy transfer occurs, and the fluorescence emission of these residues is quenched (Ren & Giusti, 2021; Zhang et al ., 2021b). Equation (3) was used to compute Ka (Zhang et al ., 2017): where F 0 and F are the fluorescence intensities of the protein at the maximum emission wavelength in the absence and presence of the ligand respectively.…”

“…All protein fluorescence spectroscopy analyses were performed using a Cary Eclipse Fluorescence Spectrometer (Varian, Bromma, Sweden) according to the method of Zhang et al . (2021b). βLG solution (0.1 m m ) and C3G (0.2 m m ) were mixed and incubated at 20 °C for 2 h in the presence and absence of ultrasound.…”

“…Zhang et al . (2021b) reported the interaction of βLG and epigallocatechin gallate (EGCG) at different temperatures, as well as the changes in antioxidant activity of the complexes. He et al .…”

Influence of ultrasound on the mechanism of conjugation between epigallocatechin gallate and β‐lactoglobulin for function improvement

“…The DPPH scavenging activity of the samples was measured according to a modified version of a previous method that 0.4 mL of 0.1 mM βLg or its 0.1 mM conjugates mixed with 3.6 mL of 0.1 mM ethanolic DPPH solution (Zhang, Li, Shao, et al ., 2021).…”

“…The structural, functional and nutritional properties of proteins could be modified by covalent binding to phenolics (Sui et al ., 2018). Our previous study showed that the conjugation of EGCG to βLg affected the Immunoglobulin E (IgE) sites of βLg leading to desensitisation while enhancing antioxidant capacity of βLg by introducing hydroxyl groups of EGCG (Zhang et al ., 2021). Meanwhile, the complex conjugation, which is influenced by many factors, is found to be highly dependent on the structures of phenolics and reaction conditions, especially temperature, leading to low conjugation efficiency (Zhang, Li, Shao, et al ., 2021).…”