Probing the dynamic crosstalk of lysosomes and mitochondria with structured illumination microscopy

Xu, Xiuqiong; Qiu, Kangqiang; Tian, Zhiqi; Aryal, Chinta; Rowan, Fiona; Chen, Rui; Sun, Yujie; Diao, Jiajie

doi:10.1016/j.trac.2023.117370

Cited by 4 publications

(6 citation statements)

References 127 publications

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“…The xanthene probe Me-hNR was produced by a condensation reaction between intermediates I and III in the presence of perchloric acid. All intermediates and the final product were well characterized by 1 HNMR, 13 CNMR, and HR-MS (Figures S1−S12). Me-hNR molecules contained delocalized positive charges and were expected to have mitochondria targeting.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.analchem.3c05154. Materials and methods; 1 HNMR, 13 CNMR, and HRMS spectra; photophysical data; stability data; and imaging data (PDF)…”

Section: * Sı Supporting Informationmentioning

confidence: 99%

“…1 H NMR (400 MHz, Chloroform-d) δ (ppm): 7.93 (d, J = 8.8 Hz, 1H), 6.57 (d, J = 8.8 Hz, 1H), 6.36 (s, 1H), 3.44 (q, J = 6.8 Hz, 4H), 2.87 (t, J = 5.6 Hz, 2H), 2.57 (t, J = 6.1 Hz, 2H), 2.10 (p, J = 5.5 Hz, 2H), 1.22 (t, J = 6.9 Hz, 6H). 13 Synthesis of Intermediate II. 3-Hydroxy-N,N-diethylaniline (1.0 g, 6.06 mmol), and CH 2 (COOPh) 2 (1.55 g, 6.06 mmol) were added to anhydrous toluene (20 mL).…”

Section: ■ Conclusionmentioning

confidence: 99%

“…1 H NMR (400 MHz, DMSO-d6) δ (ppm): 11.93 (s, 1H), 7.56 (d, J = 8.8 Hz, 1H), 6.67 (d, J = 8.9 Hz, 1H), 6.45 (s, 1H), 5.25 (s, 1H), 3.43 (q, J = 6.5 Hz, 4H), 1.13 (t, J = 6.6 Hz, 6H). 13 (20 mL). The mixture was stirred at 140 °C for 5 h. After the mixture cooled to room temperature, ammonia was added to the mixture to adjust the pH to 7.…”

Section: ■ Conclusionmentioning

confidence: 99%

“…The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.analchem.3c05154. Materials and methods; 1 HNMR, 13…”

Section: * Sı Supporting Informationmentioning

confidence: 99%

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Ultrabright Xanthene Fluorescence Probe for Mitochondrial Super-Resolution Imaging

Wu,

Zhang,

Sha

et al. 2024

Anal. Chem.

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Mitochondria are important organelles that provide energy for cellular physiological activities. Changes in their structures may indicate the occurrence of diseases, and the super-resolution imaging of mitochondria is of great significance. However, developing fluorescent probes for mitochondrial super-resolution visualization still remains challenging due to insufficient fluorescence brightness and poor stability. Herein, we rationally synthesized an ultrabright xanthene fluorescence probe Me-hNR for mitochondria-specific superresolution imaging using structured illumination microscopy (SIM). The rigid structure of Me-hNR provided its ultrahigh fluorescence quantum yield of up to 0.92 and ultrahigh brightness of up to 16,000. Occupying the para-position of the O atom in the xanthene skeleton by utilizing the smallest methyl group ensured its excellent stability. The study of the photophysical process indicated that Me-hNR mainly emitted fluorescence via radiative decay, and nonradiative decay and inter-system crossing were rare due to the slow nonradiative decay rate and large energy gap (ΔE st = 0.55 eV). Owing to these excellent merits, Me-hNR can specifically light up mitochondria at ultralow concentrations down to 5 nM. The unprecedented spatial resolution for mitochondria with an fwhm of 174 nm was also achieved. Therefore, this ultrabright xanthene fluorescence probe has great potential in visualizing the structural changes of mitochondria and revealing the pathogenesis of related diseases using SIM.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: * Sı Supporting Informationmentioning

confidence: 99%

Section: ■ Conclusionmentioning

confidence: 99%

Section: ■ Conclusionmentioning

confidence: 99%

“…The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.analchem.3c05154. Materials and methods; 1 HNMR, 13…”

Section: * Sı Supporting Informationmentioning

confidence: 99%

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Ultrabright Xanthene Fluorescence Probe for Mitochondrial Super-Resolution Imaging

Wu,

Zhang,

Sha

et al. 2024

Anal. Chem.

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Near‐Infrared Bioimaging Using Two‐photon Fluorescent Probes

Pragti,

Kundu,

Chen

et al. 2024

Adv Healthcare Materials

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Near‐infrared (NIR) bioimaging has emerged as a transformative technology in biomedical research. Among many fluorescent probes that are suitable for NIR imaging studies, two‐photon absorption (TPA) ones represent a particularly promising category, because TPA fluorescent probes can overcome the inherent limitations of one‐photon absorption (OPA) counterparts. By leveraging the unique properties of two‐photon absorption, TPA fluorescent probes achieve superior tissue penetration, significantly reduced photodamage, and enhanced spatial resolution. This perspective article delves into the fundamental principles, design strategies, and representative TPA probes for various imaging applications. In particular, a number of molecular fluorescent probes, ranging from organic, inorganic, and COF/MOF‐based systems are highlighted to showcase the vast scope of possible TPA probe design and application scenarios. In addition, the employment of stimulated TPA probes that are responsive to different external factors, including pH, redox species, enzymes, and hypoxia, is also discussed. In the end, the future perspectives for the continuous advancement of TPA fluorescent probes in the NIR bioimaging field are presented. For instance, it is essential to transition from cellular to in vivo imaging studies to obtain more physiologically relevant insights. Additionally, the development of “dual‐function” TPA probes for both disease diagnosis and therapeutic treatment is particularly promising.

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Recent advances in single fluorescent probes for monitoring dual organelles in two channels

Yang,

Yao,

et al. 2024

Smart Molecules

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Organelles are specialized areas where cells perform specific processes necessary for life and actively communicate with each other to keep the whole cell functioning. Disorders of the organelle networks are associated with multiple pathological processes. However, clearly and intuitively visualizing the highly dynamic interactions between ultrafine organelles is challenging. Fluorescence imaging technology provides opportunities due to the distinct advantages of facile, non‐invasiveness and dynamic detection, making it particularly well‐suited for applications in uncovering the mysterious veil of organelle interactions. Regrettably, the lack of ideal fluorescence agents has always been an obstacle in imaging the intricate behaviors of organelles. In this review, we provide a systematic discussion on the existing dual‐color and dual‐targetable molecular sensors used in monitoring organelle interactions, with a specific focus on their targeting strategies, imaging mechanisms and biological applications. Additionally, the current limitations and future development directions of dual‐targetable probes and dual‐emissives are briefly discussed. This review aims to provide guidance for researchers to develop more improved probes for studying organelle interactions in the biomedical field.

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Probing the dynamic crosstalk of lysosomes and mitochondria with structured illumination microscopy

Cited by 4 publications

References 127 publications

Ultrabright Xanthene Fluorescence Probe for Mitochondrial Super-Resolution Imaging

Ultrabright Xanthene Fluorescence Probe for Mitochondrial Super-Resolution Imaging

Near‐Infrared Bioimaging Using Two‐photon Fluorescent Probes

Recent advances in single fluorescent probes for monitoring dual organelles in two channels

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