Probing the interaction of troxerutin with transfer RNA by spectroscopic and molecular modeling

Subastri, Ariraman; Ramamurthy, Chitteti; Arumugam, Suyavaran; Rao, P. L. K. M.; Babu, E. Preedia; Krishna, K. Hari; Kumar, Mithilesh; Thirunavukkarasu, Chinnasamy

doi:10.1016/j.jphotobiol.2015.09.013

Cited by 7 publications

(2 citation statements)

References 45 publications

(48 reference statements)

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“…TXER also exhibits various biological activities like, anti‐fibrinolytic, anti‐erythrocytic, antithrombotic (Boisseau et al, ), anti‐oxidant (Kessler, Ubeaud, Walter, Sturm, & Jung, ), anti‐inflammatory (Fan et al, ), radioprotective (Lee et al, ), and chemopreventive effects against colon carcinogenesis (Vinothkumar et al, ). Subsequently, we have reported that TXER interacts with DNA and tRNA through groove binding and external binding mode, and exhibits antimutagenic property (Subastri et al, ; Subastri, Ramamurthy, Suyavaran, Lokeswara Rao, et al, ; Subastri, Ramamurthy, Suyavaran, Mareeswaran, et al, ). Thomas et al () proved the antineoplastic effect of TXER against liver cancer.…”

Section: Introductionmentioning

confidence: 99%

Troxerutin with copper generates oxidative stress in cancer cells: Its possible chemotherapeutic mechanism against hepatocellular carcinoma

Subastri

Arumugam

Babu

et al. 2017

Journal Cellular Physiology

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Troxerutin (TXER) a rutin derivative is known for its anticancer effect against hepatocellular carcinoma (HCC). As part of large study, recently we have shown TXER interact with genetic material and its anti-mutagenic property. In the present study we have explored its possible mode of action in HCC. Since TXER alone did not show significant anticancer effect on Huh-7 cells, in vitro biochemical assays were performed for determining anticancer efficacy of TXER + metal complex using transition metals such as Cu, Zn, and Fe. The anticancer efficacy of TXER + Cu on Huh-7 cells were evaluated using MTT assay, DCFDA, JC-1 staining, comet assay, cell cycle analysis, immunocytochemistry, and Western blotting. Non-toxic nature of TXER was analyzed on primary rat hepatocytes. The in vivo efficacy of TXER was tested in N-nitrosodiethylamine initiated and γ-benzene hexachloride and partial hepatectomy promoted rat liver cancer. Liver markers, transition metal levels, histopathological examination, and expression levels of GST-P, 8-OHdG and Ki-67 were studied to assess the in vivo anticancer effect of TXER. We observed that TXER + Cu induced extensive cellular death on Huh-7 cells through generating free radicals and did not possess any toxic effect on normal hepatocytes. The in vivo studies revealed that TXER possess significant anti-cancer effect as assessed through improved liver markers and suppressed GST-P, 8-OHdG, and Ki-67 expression. TXER treatment reduced the hepatic Cu level in cancer bearing animals. Current study brings the putative mechanism involved in anti-cancer effect of TXER, further it will help to formulate phytoconstituents coupled anti-cancer drug for effective treatment of HCC.

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Section: Introductionmentioning

confidence: 99%

Troxerutin with copper generates oxidative stress in cancer cells: Its possible chemotherapeutic mechanism against hepatocellular carcinoma

Subastri

Arumugam

Babu

et al. 2017

Journal Cellular Physiology

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“…So far, considerable works have been focused on the pharmaceutical properties of troxerutin in order to further to understand its biological activity (Lu et al, 2011;Fan et al, 2009;Maurya et al, 2005). Subastri et al (2015) reported its interaction with transfer RNA and revealed that it may have potential therapeutic application. Heidarzadeh et al (2014) showed that troxerutin may reduce tissue injury and lipid peroxidation.…”

Section: Introductionmentioning

confidence: 99%

Binding Mechanism of Bovine Serum Albumin to Troxerutin by Synchronous Fluorescence Spectroscopy and Chemometrics

Wang¹,

Yan²,

Luo³

2016

American Journal of Biochemistry and Biotechnology

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Previous works have reported the binding of Bovine Serum Albumin (BSA) to troxerutin by absorption and fluorescence spectroscopy. In order to extend the investigation of their binding characteristics, the study presented here was designed to evaluate their interaction by the synchronous fluorescence spectroscopy and Multivariate Curve Resolution With Alternating Least Squares (MCR-ALS) under simulative physiological conditions. The Evolving Factor Analysis (EFA) and MCR-ALS analysis results show that there are three chemical species in the troxerutin-BSA system, which reveal that the fluorescence quenching of BSA caused by troxerutin may be a static quenching mechanism. The spectral profile of each compound was obtained by the soft of MCR-ALS. The obtained average binding constant is 6.43×10 5 mol −1 L for BSA and troxerutin concentration ratio is 7 while that is 6.89×10 5 mol −1 L for troxerutin and BSA concentration ratio is 5. The study might further help us to better understand the structural features and toxicological action of troxerutin at the molecular level.

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Metal Oxide Nanoparticles Having Strong Physico- and Electrochemical Interaction with Genetic Material Will Have Better Anticancer Efficacy

Sujatha,

Kaviyasri,

Guha

et al. 2024

BioNanoSci.

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Probing the interaction of troxerutin with transfer RNA by spectroscopic and molecular modeling

Cited by 7 publications

References 45 publications

Troxerutin with copper generates oxidative stress in cancer cells: Its possible chemotherapeutic mechanism against hepatocellular carcinoma

Troxerutin with copper generates oxidative stress in cancer cells: Its possible chemotherapeutic mechanism against hepatocellular carcinoma

Binding Mechanism of Bovine Serum Albumin to Troxerutin by Synchronous Fluorescence Spectroscopy and Chemometrics

Metal Oxide Nanoparticles Having Strong Physico- and Electrochemical Interaction with Genetic Material Will Have Better Anticancer Efficacy

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