Probing the role of scaffold dimensionality and media composition on matrix production and phenotype of fibroblasts

Chawla, Smriti; Chameettachal, Shibu; Ghosh, Sourabh

doi:10.1016/j.msec.2015.01.059

Cited by 13 publications

(10 citation statements)

References 40 publications

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“…Although the stemness maintenance of stem cells is supported by the 3D geometry of the matrix, changes in cell and nuclear volumes during the transition from 2D to 3D microenvironments are independent of material stiffness . These findings imply the importance of a 3D structure on cell fate determination, but the reciprocal interaction between the internal dimensionality of biomaterials and resident cells remains largely unexplored …”

Section: Engineering a 3d Home For Cell Accommodationmentioning

confidence: 97%

Engineering a Cell Home for Stem Cell Homing and Accommodation

Yin

et al. 2017

Adv. Biosys.

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Distilling complexity to advance regenerative medicine from laboratory animals to humans, in situ regeneration will continue to evolve using biomaterial strategies to drive endogenous cells within the human body for therapeutic purposes; this approach avoids the need for delivering ex vivo‐expanded cellular materials. Ensuring the recruitment of a significant number of reparative cells from an endogenous source to the site of interest is the first step toward achieving success. Subsequently, making the “cell home” cell‐friendly by recapitulating the natural extracellular matrix (ECM) in terms of its chemistry, structure, dynamics, and function, and targeting specific aspects of the native stem cell niche (e.g., cell–ECM and cell–cell interactions) to program and steer the fates of those recruited stem cells play equally crucial roles in yielding a therapeutically regenerative solution. This review addresses the key aspects of material‐guided cell homing and the engineering of novel biomaterials with desirable ECM composition, surface topography, biochemistry, and mechanical properties that can present both biochemical and physical cues required for in situ tissue regeneration. This growing body of knowledge will likely become a design basis for the development of regenerative biomaterials for, but not limited to, future in situ tissue engineering and regeneration.

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Section: Engineering a 3d Home For Cell Accommodationmentioning

confidence: 97%

Engineering a Cell Home for Stem Cell Homing and Accommodation

Yin

et al. 2017

Adv. Biosys.

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“…Metabolic activity was estimated using the MTT ((3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyltetrazolium bromide)) assay (CT01‐5, Millipore, India) as described before (Chawla, Chameettachal, & Ghosh, ). Briefly, MTT was added in 1:10 ratio (MTT: culture media) for 3 hr at 37°C followed by dissolution with dimethyl sulphoxide (Merck, India) and absorbance was measured using iMark microplate absorbance reader (BIORAD, India) at 560 nm.…”

Section: Methodsmentioning

confidence: 99%

“…Immunofluoroscence was carried out at days 3 and 14 on formalin‐fixed samples (Chawla et al, ). Primary antibodies for anti‐collagen I (4 µg/ml, Millipore), anti‐SPARC (0.25 µg/5µl, BD Biosciences, India), anti‐Tenascin (2 µg/ml, Sigma, India), anti‐α‐SMA (2 µg/ml, eBioscience, Thermo Fisher, India) with their respective secondary antibodies, Alexa Fluor 488 (Invitrogen), Alexa Fluor 546 (Invitrogen) were used.…”

Section: Methodsmentioning

confidence: 99%

Establishment of in vitro model of corneal scar pathophysiology

Chawla

Ghosh

2017

Journal Cellular Physiology

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Corneal scarring is the major source of permanent blindness worldwide. The complex pathophysiology of corneal scarring is not comprehensibly understood as it involves the interaction of a constellation of pro-fibrotic cytokines influencing several signaling pathways involved in corneal scar development. In the present study, an attempt has been made to generate a relatively simple in vitro corneal scar model using primary corneal keratocytes by exogenously providing an optimized dose of combination of cytokines (TGF-β1, IL-6, and IL-8) involved in scar formation in situ. Data obtained from gene and protein expression analysis depicted enhanced ECM production with discrete expression of myofibroblast specific markers. The protein-protein interactions associated these proteins to various pathways involved in wound healing, cellular migration, and cytoskeletal remodeling justifying high relevance to in vivo scar formation. Hence the developed model can be used to acquire understanding about corneal scar pathophysiology and thus might be useful for designing the treatment modalities and efficacies for controlling scar formation.

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“…no. 204074; Qiagen) and the Rotor Gene Q thermocycler (Qiagen; Chawla, Chameettachal, & Ghosh, 2015). QuantiTect Primers (Qiagen) listed in Table 1 were used for the gene expression analysis.…”

Section: Gene Expression Analysis By Reversetranscription Polymerasmentioning

confidence: 99%

Establishment of an in vitro organoid model of dermal papilla of human hair follicle

Gupta

Chawla

Hegde

et al. 2018

Journal Cellular Physiology

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Human hair dermal papilla (DP) cells are specialized mesenchymal cells that play a pivotal role in hair regeneration and hair cycle activation. The current study aimed to first develop three-dimensional (3D) DP spheroids (DPS) with or without a silk-gelatin (SG) microenvironment, which showed enhanced DP-specific gene expression, resulting in enhanced extracellular matrix (ECM) production compared with a monolayer culture. We tested the feasibility of using this DPS model for drug screening by using minoxidil, which is a standard drug for androgenic alopecia. Minoxidil-treated DPS showed enhanced expression of growth factors and ECM proteins. Further, an attempt has been made to establish an in vitro 3D organoid model consisting of DPS encapsulated by SG hydrogel and hair follicle (HF) keratinocytes and stem cells. This HF organoid model showed the importance of structural features, cell-cell interaction, and hypoxia akin to in vivo HF. The study helped to elucidate the molecular mechanisms to stimulate cell proliferation, cell viability, and elevated expression of HF markers as well as epithelial-mesenchymal crosstalks, demonstrating high relevance to human HF biology. This simple in vitro DP organoid model system has the potential to provide significant insights into the underlying mechanisms of HF morphogenesis, distinct molecular signals relevant to different stages of the hair cycle, and hence can be used for controlled evaluation of the efficacy of new drug molecules.

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Probing the role of scaffold dimensionality and media composition on matrix production and phenotype of fibroblasts

Cited by 13 publications

References 40 publications

Engineering a Cell Home for Stem Cell Homing and Accommodation

Engineering a Cell Home for Stem Cell Homing and Accommodation

Establishment of in vitro model of corneal scar pathophysiology

Establishment of an in vitro organoid model of dermal papilla of human hair follicle

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